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Kirby Bauer

QuestionAnswer
What factors influence the results of the Kirby Bauer testing? Freshness of the Mueller- Hinton (MH) agar, Agar depth (4mm), Drying time, proper incubation, purity of the inoculum, pH (7.2-7.4), inoculum size, thymidine-free, and calcium and magnesium supplemented
How would you report a result that has a thin veil inside the zone of inhibition around the disk? This is typical of Proteus (mirabilis or vulgaris) due to swarming. Therefore, that should be ignored and the outer margin should be measured
What is the principle of the Kirby-Bauer method? It's the disk diffusion method of susceptibility testing that utilizes small circles or disks of filter paper impregnated with standard quantities of various antimicrobial agents.
When is it appropriate to use the Kirby Bauer method? The Kirby-Bauer method is used on organisms that fail to grow on the MIC panels
What are the common methods used to detect methicillin resistant Stahylococci? 1. Cefoxitin antibiotic in place of oxacillin because it is more sensitive 2. Penicillin-Binding-Protein 2
What is the concentration of NaCl in the broth and agar method used to enhance the detection of methicillin resistance of Staphylococci? 2% NaCl to broth 4% NaCl to agar
When is the induction test used and what is the procedure? It's used when Erythromycin appears resistant and Clindamycin appears susceptible. A MH agar is streaked, the disks placed 15-26mm apart and incubated overnight. A flattened "D" will appear around Clindamycin if Erthromycin induced restistance.
When the antibiotic Cefoxitin is used, what is the size of the zone of inhibition that defines MRSA? Less than or equal to 21mm
What are some factors that can cause a falsely decreased zone sizes? The Mueller-Hinton agar depth is greater than 4mm, us of deteriorated disks, an increase in the concentration of calcium and magnesium, using a McFarland standard greater than 0.5 turbidity, a 2 hour delay in placing the antimicrobial disk on the plate
How are the production of Beta lactamaes by bacteria detected? Chromogenic cephalosporin (Nitrocefin) technique. A positive test predicts that an isolate will not respond to many Beta-lactam antibiotics. Iodometric and acidimetric tests may also be used
Created by: leahneese
 

 



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