Help
Options
Didn't know it?
click below
click below
Knew it?
click below
click below
Don't Know
Remaining cards (0)
Know
retry
shuffle
restart
0:00
Embed Code - If you would like this activity on your web page, copy the script below and paste it into your web page.
Normal Size Small Size show me how
Normal Size Small Size show me how
Decalcification
Special technique in processing
| Question | Answer |
|---|---|
| The process of removing calcium from bone or tissue, thus allowing routine microtomy on paraffin-embedded material is the definition of what? | Decalcification |
| Faliure to decalcify tissue with large amounts of calcium will result in? | Torn or ragged sections, damage to the cutting edge of the microtome. |
| What are two routine methods of decalcification? | Acid Method and Chelating Method (all other methods are modifications of these) |
| What are the three acid methods of decalcification? | Simple acid method, Ion exchange method, and Electrolytic method. |
| Calcium salts are dissolved and then ionized describes Acid Method or Chelating Method? | Acid Method |
| The WAY in which calcium ions are removed from tissue is the basis of which method of decalcification, Acid Methods or Chelating Method? | Acid Methods |
| This acid method of decalcification utilizes a mixture of formic and hydrochloric acid? placed in an apparatus based on a simple electroplating devise. | Electrolytic Method |
| With this acid method of decalcification, the bone is attached to the +anode(positive pole) and a current is passed through the solution. The calcium ions(+charge) are attracted away from the anode toward the -cathode(negative pole)? | Electrolytic Method |
| This acid method of decalcification is rarely used today, it takes 2-6 hours to process, a limited number of specimans can be processed at a time and heat generated has a potential for tissue destruction and a total loss of cell detail and stainability? | Electrolytic Method |
| In this acid method of decal, calcium ions are allowed to migrate out of the tissue into the surrounding solution. The solution around the tissue may become saturated with calcium ions, almost forming a barrier to further decal? | Simple Acid Method |
| This method of acid decal requires the speciman to be suspended in an embedding bag to expose all surfaces to the action of the decal fluid and allows precipitated calcium salt to sink? | Simple Acid Method |
| This acid method of decalcification is rarely used today, it takes 2-6 hours to process, a limited number of specimans can be processed at a time and heat generated has a potential for tissue destruction and a total loss of cell detail and stainability? | Electrolytic Method |
| This acid method of decalcification is considered the best method for decalcification. | Ion Exchange Resins |
| This method of acid decal involves the use of formic acid over a layer of an ammoniated salt of a sulfonated resin. Ammonium ions from the resin are exchanged for calcium ions; this keeps the solution free of calcium ions and speeds up the reaction? | Ion Exchange Resins |
| In this acid method of decal, calcium ions are allowed to migrate out of the tissue into the surrounding solution. The solution around the tissue may become saturated with calcium ions, almost forming a barrier to further decal? | Simple Acid Method |
| This method of acid decal requires the solution to be changed frequently and agitate. Vacuuming at the initial stage will aid in infiltrating the speciman with decal fluid and will draw off carbon dioxide bubbles that form on the spicimans surface? | Simple Acid Method |
| In this method of decal, a ring is formed by joining an agent to a metal ion. These agents are organic. An example is EDTA which decals by binding ions. pH is 5.0-7.2 and is a very slow method? | Chelating Method |
| The most important step in the decal process is? | End point of decalcification |
| Undercalcification of tissue reults in.... while overdecalcification of tissue results in...? | underdecalcied tissue will section with difficulty and overdecalcified tissue will stain poorly. |
| What are the three basic methods of end point decal? | Radiographic, Chemical, and Mechanical (or Physical) |
| This is the most desirable methos of end point decal because its easy to use and yeilds visual evidence that demineralization is complete? | Radiography method of end point decal |
| This type of end point for decal cannot be used on metallic fixed tissue such as Zenker or B-5 because metal will reder the speciman radiopague? | Radiography method of end point decal |
| This most desirable method of end point decal uses small x-ray units that are easy to use and are available in most histo. labs. | Radiography method of end point decal |
| This method of end point decal depends on the precipitation od calcium oxalte. You mix a sample of the used decal solution with a solution of ammonium hydroxide and ammonium oxalate and let stand for 30 minutes? | Chemical method of end point decal |
| With this method of end point decal the presence of calcium is indicated if the solution remains turbid and you keep retesting the solution until it is free of calcium? | Chemical method of end point decal |
| This method of end point decal is the least desirable, is inaccurate and can possibly create artifacts. It involves testing the flexability of the speciman by probing it with a needle or pin and scraping the section surfaces? | Mechanical (or physical) method of end point decal |
| The most frequently used embedding media for undecalcified bone is? | Glycol Methacrylate |
| For undecalcified bone, section of bone may be ground with waterproof sandpaper then stained and mounted on glass slides, how many microns thick should they be? | 75 -100 microns thick |
| Metallic fixatives interfere with most fixative techniques of undecalcified bone, what are three fixatives of choice? | Alcohol, Buffered Formalin, or Calcium Formalin |
Created by:
Pixel