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Which of the following statements about libraries is true?
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What is the major difference between the DNA sequencing techniques developed by Sanger and others in the 1970s and next-generation sequencing methods?
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Chapter 12 and 13

QuestionAnswer
Which of the following statements about libraries is true? A cDNA library constructed from human brain tissue will likely be different from a cDNA library constructed from human pancreatic tissue.
What is the major difference between the DNA sequencing techniques developed by Sanger and others in the 1970s and next-generation sequencing methods? Unlike the earlier technology, next-generation sequencing relies heavily on miniaturization techniques
Two-dimensional gel electrophoresis separates proteins based on _______ and _______. size; charge
Which of the following enzymes perform opposite functions? Restriction endonuclease and DNA ligase
Which organismal group exhibits the most compact genomes? bacteria
Which of the following statements about eukaryotic genomes is false? The percentage of the genome devoted to coding sequence is higher than in prokaryotic genomes.
Which of the following necessarily makes use of reverse transcriptase? Construction of a cDNA library
When restriction enzymes make cuts with _______ ends, the DNA sequences can form _______ bonds with complementary sequences. sticky; hydrogen
Which of the following statements about the Human Genome Project (HGP) is false? Only privately funded groups were involved in the sequencing effort.
Which is not true of the "Spider Goat" lecture example? production of silk from cells in culture was successful
pGLO would be used in gene cloning
The DNA from the genome of an organism is chopped into numerous fragments, which then are inserted into vectors and cloned. The genetic information that is present in the resulting colonies is called a genomic library.
DNA sequencing is most like the natural process of DNA replication.
Which of the following genomes is most likely that of a free-living prokaryote? A genome that is 3 million bp long, arranged in a single circular chromosome, and has little repetitive DNA
Which of the following statements about genomes is true? Most genes found in the genomes of complex multicellular organisms are not required for survival.
In order for a protein to be made from its introduction into a host cell, ____ are needed. expression vectors
In next-generation DNA sequencing technology, the fluorescent dye labels each of the four nucleotides with a different marker.
Which of the following statements about dogs and dog genomes is false? Dogs vary tremendously in size and shape because they were domesticated from many different, and different looking, species of wolves.
"Roundup-ready" crops have allowed for the development of no till planting. b. incorporated the gene for glyphosate resistance. c. saved farmers money on tractor fuel. d. All of these are correct
Which of the following statements about yeasts is false? They are always haploid
The polymerase chain reaction amplifies specific DNA sequences
Which of the followin statements about Mycoplasma genitalium is not true? It has a wide array of regulatory genes, many of which other bacteria lack
Which of the following characteristics would make an organism more likely to be a model organism? Ease of rearing in the lab
Which of the following statements about the globin genes in humans is false? they are all expressed at the same time
YACs would used in yeast
In order for a protein to be made from its introduction into a host cell,____ are needed. expression vectors
which are the following genomes is most likely that a free-living prokaryote? a genome that is 3 million bp long, arranged in a single circular chromosomes, and has little repetitive DNA
a study comparing the proteins that are produced in sperm cells to those of skin cells would be an example proteomics
which of the following is required for the formation of gene famiies gene duplication
Rank the following in terms of their relationship to phenotypic characteristics (from closest tomost distant): the genome, the proteome, and the metabolome. Metabolome; proteome; genome
The maximum length of DNA that can be sequenced at one time by means of the standardtechnology is about _______ bases. 700
DNA, because it has a _______ charge, moves to the _______ end of the field in gelelectrophoresis; _______ DNA molecules migrate the most quickly. negative; positive; smaller
In next-generation DNA sequencing technology, the PCR technique produces many identical copies of specific DNA fragments
Created by: kelly098765
 

 



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