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Micro Lab Exercise 1

QuestionAnswer
Consisted of biconvex lenses and were essentially magnifying glasses. Simple Microscope
Shows dark objects in a bright field. Used most often. Brightfield Compound Microscope
The larger knob used for focusing with the low-power objectives (4x and 10x). It moves the lenses or the stage longer distances. Coarse Adjustment
The smaller knob is used for focusing with the high-power and oil immersion lenses. Fine Adjustment
The area seen through a microscope. Field of vision
The most important lens in microbiology. It has the highest magnification and must be used with immersion oil. Oil immersion lens
Remagnifies the image formed by the objective lens. Ocular Lens (eyepiece)
Transmits the image from the objective lens to the ocular lens. Body Tube
Primary lenses that magnify the specimen. Objective lenses
Holds the microscope slide. Mechanical stage
Focuses light through specimen. Condenser
Controls the amount of light entering the condenser. Diaphragm
Light source Illuminator
Used for focusing the specimen; turning the knob changes the distance between the objective lens and the specimen. Fine Focusing Knob
The ability of a lens to revel fine detail or two points distinctly separated. Resolution or Resolving Power
The amount the light bends. Refractive index
The three basic shapes of bacteria. 1. Bacillus (plural: bacilli) or Rod 2. spiral 3. Coccus (plural: cocci)
The high-dry lens will be optimal for observing multicellular fungi or algae? True
The oil immersion objective is necessary to determine the morphology of prokaryotes? True
What three bacterial shapes do you observe under a microscope? Bacillus, cocci, spiral
How does increased magnification affect the field of vision? The field of view gets smaller.
Which objective focuses closest to the slide when it is in focus? Oil immersion
Name two ways in which you can enhance the resolving power. 1. Adjust the coarse adjustment. 2. Oil immersion.
What are the advantages of low-power objective over the oil immersion objective for viewing fungi or algae? Larger field of view
What would occur if water were accidentally used in place of immersion oil? Less resolution. More light would escape.
The total magnification of the object is calculated by multiplying the magnification of the ocular (usually 10x) by the magnification of the objective lens. True
Which objective lens is red and has a power of 4X (4x10=40)? The scanning objective lens
Which objective lens is yellow and has a power of 10X (10x10=100)? The low-power objective lens
Which objective lens is blue and has a power of 40X (40X10=400)? The high-dry objective lens
Which objective lens has the highest magnification at 100X (100X10=1000) and must be used with immersion oil? The oil-immersion objective lens
Created by: KamiSeago
 

 



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