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Lab Procedures Q1

QuestionAnswer
What type of hemolysis creates a narrow band of greenish or slimy discoloration around the bacterial colony? Alpha-hemolysis
What type of hemolysis creates a clear zone around the bacterial colony? Beta-hemolysis
What type of hemolysis produces no change in the appearance of the medium and no hemolysis around colonies? Gamma-hemolysis
What is another name for double-zone hemolysis? Delta-hemolysis
What type of media is formulated to meet the requirements of the most fastidious pathogens? enriched media
What are some examples of enriched media? Blood Agar, chocolate Agar
What type of media contains antibacterial substances such as bile salts or antimicrobials that inhibit or kill all but a few types of bacteria? Selective media
What is an example of selective media? McConkey Agar
What is an example of a differential media? Simmons citrate
What type of media allows bacteria to be differentiated into groups based on their biochemical reactions? Differential media
What type of media is a liquid media that favors the growth of a particular organism, or may contain inhibitory substances that supress that organism's competitors? enrichment media
What types of medium does blood Agar function as? enrichment, and differential
What types of medium are MacConkey Agar and Eosin-methylene Blue Agar? selective and differential
What type of bacteria is thioglycollate broth used to culture and why? anaerobic, it helps to determine their oxygen tolerance with a stable oxygen gradient from the surface to the bottom
What is the incubation temp. for bacteria cultures? 37 C
What type of media is Mueller-Hinton Agar? general purpose media
What type of techinique must be used when collecting samples for microbiology testing? aseptic technique
What is the most common cause of diagnostic failure in microbiology testing? specimen contamination
What type of test media will most Gram-positive and Gram-negative bacteria grow in? blood Agar, Gram-postive do not grow in MacConkey Agar
How long should bacteria cultures be incubated before examination? examine at 18-24 hours, continue incubating for up to 48 hours for routine cultures
Created by: Marrok359
 

 



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