Question 1

5 catagories of biochemical tests

Accepted Answer

-Fermentation
-Hydrolytic enzymes
-Amino acid utilization
-Citrate utilization
-Miscellaneous and Combined tests

Question 2

types of fermentation biochemical tests

Accepted Answer

-Phenol Red broth
-Methyl Red (MR) and Voges-Proskauer (VP)
-ONPG

Question 3

contents of phenol red test

Accepted Answer

-Contains peptone broth with a pH indicator
(Phenol Red)
-One sugar per tube

Question 4

Phenol Red: Positive- ___ Negative-_____

Accepted Answer

POS: Yellow// NEG: Red

Question 5

___ can be added to a phenol red test to detect gas production

Accepted Answer

Durham Tube

Question 6

Methyl Red/Voges-Proskauer (MRVP): 
-Determines organism’s ability to produce _____ end products from glucose fermentation

Accepted Answer

stable acid

Question 7

MRVP tests Grow the organism in _____ broth for 24 hours

Accepted Answer

brain-heart infusion (BHI)

Question 8

Methyl Red Test: Pos: ___ Neg: ____

Accepted Answer

Pos: Red // Neg: Yellow

Question 9

Voges-Proskauer (VP):
Detects organisms ability to convert acid products to ___ and ___

Accepted Answer

acetoin and 2,3-butanediol

Question 10

VP Test: POS- ___ NEG-____

Accepted Answer

POS: RED// Neg: Yellow

Question 11

ONPG (o-Nitrophenyl-β-D-Galactopyranoside):
Detects the ability of an organism to produce ________

Accepted Answer

β-galactosidase

Question 12

OPNG: Distinguishes late_____ from ______

Accepted Answer

lactose fermenters from non-lactose fementers

Question 13

OPNG positive color

Accepted Answer

Yellow

Question 14

Hydrolytic Enzymes
Detects ability to hydrolyze certain compounds such as:

Accepted Answer

-Indole _Urease

Question 15

Indole
Identifies organisms that produce the enzyme ________

Accepted Answer

tryptophanase

Question 16

Positive Indole test

Accepted Answer

Red (pink)  color

Question 17

Urease:
Determines organism’s ability to produce urease
Urease hydrolyzes urea to __ and ____

Accepted Answer

ammonia and CO2

Question 18

Deamination tests

Accepted Answer

Phenylalanine deaminase

Question 19

Decarboxylation Tests

Accepted Answer

-Lysine decarboxylase
-Ornithine decarboxylase
-Arginine dihydrolase or decarboxylase

Question 20

Determines if an organism can deaminate phenylalanine

Accepted Answer

Phenylalanine deaminase (PAD)

Question 21

Positive Phenylalanine test color:

Accepted Answer

Green

Question 22

Add ____ to a Phenylalanine deaminase (PAD) test

Accepted Answer

Ferric Chloride

Question 23

An amino acid is broken down to an Amine + CO2 by ____

Accepted Answer

decarboxylase

Question 24

A POS decarboxylase test will by the color ___

Accepted Answer

Purple

Question 25

Lysine:
-Enzyme is lysine decarboxylase
-Amine product is ____

Accepted Answer

cadaverine

Question 26

Ornithine:
-Enzyme is ornithine decarboxylase
-Amine product is _____

Accepted Answer

putrescine

Question 27

Arginine:
-Enzymes are_____ and arginine decarboxylase
-Amine product is______

Accepted Answer

arginine dihydrolase//  putrescine

Question 28

Citrate Utilization
Identifies organisms that can use ____  as a sole source of carbon
AND
_______ as the sole source of nitrogen

Accepted Answer

citrate// Inorganic ammonium salts

Question 29

POS citrate test color

Accepted Answer

Blue (alkaline)

Question 30

-Determines if organism ferments glucose, lactose, and/or sucrose
-Determines if organism reduces sulfur to hydrogen sulfide (H2S)

Accepted Answer

Triple Sugar Iron (TSI)

Question 31

TSI: Yellow slant/Yellow deep (A/A)

Accepted Answer

Ferments glucose and Lactose or Sucrose (or both)

Question 32

TSI: Red slant/Yellow deep (K/A)

Accepted Answer

Ferments glucose but neither lactose not sucrose

Question 33

TSI: Red slant/Red deep (K/K)

Accepted Answer

Does not ferment any of the sugars (glucose, lactose, sucrose)

Question 34

TSI: If ___ is reduced  to hydrogen sulfide, the H2S reacts with ferrous sulfate to form ferrous sulfide – a black precipitate

Accepted Answer

thiosulfate

Question 35

Nitrate Reduction
Determine ability of organism to reduce ___ to _____
-All ________ are positive

Accepted Answer

nitrates to nitrites// Enterobacteriaceae

Question 36

Nitrate Reduction:  If an organism reduces nitrate past nitrite, no color change is present, ___ is added and if there IS a color change the the reaction is (pos/neg?) No color change = (POS/NEG?)

Accepted Answer

zinc// color change = NEG// No color change = POS

Question 37

New method bacteria diagnosis using 2 mass specs

Accepted Answer

MALDI-TOF-
Matrix-Assisted Laser Desorption Ionization - Time Of Flight

Microbiology

Enterobactericea Biochemicals

Question	Answer
5 catagories of biochemical tests	-Fermentation -Hydrolytic enzymes -Amino acid utilization -Citrate utilization -Miscellaneous and Combined tests
types of fermentation biochemical tests	-Phenol Red broth -Methyl Red (MR) and Voges-Proskauer (VP) -ONPG
contents of phenol red test	-Contains peptone broth with a pH indicator (Phenol Red) -One sugar per tube
Phenol Red: Positive- ___ Negative-_____	POS: Yellow// NEG: Red
___ can be added to a phenol red test to detect gas production	Durham Tube
Methyl Red/Voges-Proskauer (MRVP): -Determines organism’s ability to produce _____ end products from glucose fermentation	stable acid
MRVP tests Grow the organism in _____ broth for 24 hours	brain-heart infusion (BHI)
Methyl Red Test: Pos: ___ Neg: ____	Pos: Red // Neg: Yellow
Voges-Proskauer (VP): Detects organisms ability to convert acid products to ___ and ___	acetoin and 2,3-butanediol
VP Test: POS- ___ NEG-____	POS: RED// Neg: Yellow
ONPG (o-Nitrophenyl-β-D-Galactopyranoside): Detects the ability of an organism to produce ________	β-galactosidase
OPNG: Distinguishes late_____ from ______	lactose fermenters from non-lactose fementers
OPNG positive color	Yellow
Hydrolytic Enzymes Detects ability to hydrolyze certain compounds such as:	-Indole _Urease
Indole Identifies organisms that produce the enzyme ________	tryptophanase
Positive Indole test	Red (pink) color
Urease: Determines organism’s ability to produce urease Urease hydrolyzes urea to __ and ____	ammonia and CO2
Deamination tests	Phenylalanine deaminase
Decarboxylation Tests	-Lysine decarboxylase -Ornithine decarboxylase -Arginine dihydrolase or decarboxylase
Determines if an organism can deaminate phenylalanine	Phenylalanine deaminase (PAD)
Positive Phenylalanine test color:	Green
Add ____ to a Phenylalanine deaminase (PAD) test	Ferric Chloride
An amino acid is broken down to an Amine + CO2 by ____	decarboxylase
A POS decarboxylase test will by the color ___	Purple
Lysine: -Enzyme is lysine decarboxylase -Amine product is ____	cadaverine
Ornithine: -Enzyme is ornithine decarboxylase -Amine product is _____	putrescine
Arginine: -Enzymes are_____ and arginine decarboxylase -Amine product is______	arginine dihydrolase// putrescine
Citrate Utilization Identifies organisms that can use ____ as a sole source of carbon AND _______ as the sole source of nitrogen	citrate// Inorganic ammonium salts
POS citrate test color	Blue (alkaline)
-Determines if organism ferments glucose, lactose, and/or sucrose -Determines if organism reduces sulfur to hydrogen sulfide (H2S)	Triple Sugar Iron (TSI)
TSI: Yellow slant/Yellow deep (A/A)	Ferments glucose and Lactose or Sucrose (or both)
TSI: Red slant/Yellow deep (K/A)	Ferments glucose but neither lactose not sucrose
TSI: Red slant/Red deep (K/K)	Does not ferment any of the sugars (glucose, lactose, sucrose)
TSI: If ___ is reduced to hydrogen sulfide, the H2S reacts with ferrous sulfate to form ferrous sulfide – a black precipitate	thiosulfate
Nitrate Reduction Determine ability of organism to reduce ___ to _____ -All ________ are positive	nitrates to nitrites// Enterobacteriaceae
Nitrate Reduction: If an organism reduces nitrate past nitrite, no color change is present, ___ is added and if there IS a color change the the reaction is (pos/neg?) No color change = (POS/NEG?)	zinc// color change = NEG// No color change = POS
New method bacteria diagnosis using 2 mass specs	MALDI-TOF- Matrix-Assisted Laser Desorption Ionization - Time Of Flight

"Know" box contains:
Time elapsed:
Retries: