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Stack #1806903
| Question | Answer |
|---|---|
| Name all electrophoretic media that seperates proteins and what the seperation is based on | Agarose gel,cellulose acetate...based on molecular size |
| The biuret method for total serum protein is used for serum but not for protein in the urine or csf because? | Low sensitivity in urine and csf |
| Protein fractions that are produced in the liver are | Albumin,A1,A2 Beta NOT GAMMA |
| peptide bonds of proteins bind with ___ions of biuret reagent to produce a colored product | Cupric c2 |
| If serum proteins were immursed in a buffer of pH 8.6 which protein Albumin or gamma globulin would migrate furthest and toward which electrode | Albumin and anode |
| Common clinical laboratory methods for measurement of serum albumin are based on the properties of albumin as a | dye binder |
| A glycoprotein that binds with hgb to facilitate the removal of hgb by the RES is | Haptaglobin |
| The acute phase reactant protein that also inhibits proteolysys and has the highest concentration within its globulin fraction is | A1 antitrypsin |
| A protein that is normally produced by the fetus but is found in increased amounts in the amniotic fluid in cases of spina bifida | AFP |
| Portal cirrhos is a chronic desease of the liver.observed on electrophoretic serum protein pattern a predominant characteristic of this disease is | B-Y(beta_gamma)chains of cirrhosis |
| increased serum albumin concentrations are seen in which of the following conditions | Dehydration |
| Elevations in which of the following are most likely to indicate liver disease | ALP,ALT,AST,LD,GGT |
| describe the difference between kinetic or rate reactions | they are the same "true" |
| What is the most sensitive enzymatic indicator for liver damage from ethanol intake | GGT |
| when myocardial infarction occurs,the first enzyme to reach peak elevation is | CK |
| ALT and AST are both elevated in which of the following diseases | Viral hepatitis |
| an elevated aldolase level and normal myoglobin is most indictive of what condition | muscular dystrophy |
| when a purified enzyme is used as a reagent the enzume must be___ concentrationso that the reaction follows___kinetics | excess,zero |
| What is the major cellular organ of acid phosphatase activity in serum | prostate |
| which of the following methods of measuring enzymy activity utilizes an increase in absorbance at 340 nm as and indicator of reaction | karmen method,spectrophotometer |
| which enzyme has multiple molecular forms whose clinical significance is used in diagnosis | LD,CK,ALP |
| one extracellular enzyme whose serum activity is measured clinically is | amylase |
| in the spectrophotometric determination of AST malic dehydrogenase catalyzes the conversion of what to what | oxalacetate to malate |
| the greatest tissue source of CK is found where | skeletal muscle |
| which enzyme detects very early changes in liver cell damage | GGT |
| what enzyme test is most effected by hemolysis | AST,LD |
| one international unit is defined as one ___ of substrate converted to product per___ | micromol,minute |
| Name all coenzymes | magnesuim is not a coenzyme |
| what enzyme requires chloride as an activator | amylase |
| the enzyme that is inhibited by hgb is | lipase |
| prostatis acid phosphatese measurement has been replaced by what test | PSA |
| the only clinically analyzed enzyme in the urine is | amylase |
| in the szasz method for gamma glutamyl transferase(GGT) the typical receptor molecule is | glycylglycine |
| a reaction rate assay measures enzyme___ an endpoint assay measures enzyme___ | activity,activity |
| during 2 glucose tests an hour apart on a patient whole blood is substituted for plasma what results would you expect | whole blood is 11% lower than plasma |
| the most common blood reducing sugar is | sucrose |
| which of the following methods for glucose is the most common | hexohinase,glucose oxidase |
| the conversion of amino acids by the liver and other sepcialized tissues to substrates that be converted to glucose is called | gluconeogenesis |
| the reference method for glucose determination is | hexokinase |
| in the beckman analyzer for glucose,h2o2 is consumed in side reactions by | catalyze and ethanol alcohol |
| a serum specimen for glucose was allowed to stand unseperated on the clot for 6 hrs at room temp what would the results be after 3 hours | 178mg/dl |
| the perferred collection tube for preservation of glucose is | sodium floride gray top |
| the following tolarance testresults are indictive of what state | diabetes mellitus |
| describe type 1 diabetes | childhood onset,insulin dependant,b cell destruction,ketoacidosis |
| what happens after the activation of insulin | increased glycogenesis and glycolysis and lipogenolysis. decreased genolysis |
| the glucose value of a normal 2 hr post prandial serum specimen as compared to the referance range for a fasting serum glucose should be | normal increased over fasting |
| which of the following increases plasma glucose and glycolysis | growth hormone |
| which lab procedure can determine glucose levels over the past 2 months | HgB A1c |
| which cardiac marker is elevated the longest period after myocardial infarction | cTn1 cardiac troponin inhibitor |
Created by:
jennsabo2010