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Biology Lab Exam 1
| Question | Answer |
|---|---|
| Microscopes. What is the function of the body tube? | Maintains proper distance between lenses |
| Microscope. What is the function of the revolving nosepiece? | Hold the objective-rotates to change magnification |
| Microscope. What is the function of the objective? | magnification. |
| Microscope. What is the function of the stage clips? | Holds the slide in place. |
| Microscope. What is the function of the light source? | reflects light towards eyepiece |
| Microscope. What is the function of the ocular lens(eyepiece)? | Magnification. |
| Microscope. What is the function of the arm? | supports body tube |
| Microscope. What is the function of the stage? | supports slide |
| Microscope. What is the function of the coarse adjustment knob? | focuses image |
| Microscope. What is the function of the fine adjustment knob? | sharpens the knob |
| Microscope. What is the function of the base? | supports microscope. |
| What are the steps of the scientific method? | Observation, hypothesis, prediction, experimentation, analysis and conclusion |
| Define observation | ask a question about the selected topic and then research the topic |
| Define hypothesis | A proposed explanation ( a educated guess) |
| Define prediction | Assume that the hypothesis is correct and then predict what the result of testing will be |
| Define inductive reasoning | logical process that involves looking at all known observations and considering them all. Moves from specific to general |
| Define deductive reasoning | a prediction is produced from a possible answer to the question. Moves from general to specific. |
| Define experimental group | group that is treated with the independent variable. |
| Define Independent variable | treatment or condition under study. The variable you can control or manipulate. |
| Define dependent variable | the variable that depends on the independent variable |
| Define controlled variable | is anything that can affect the dependent variable. All other factors that the researcher tries to hold constant. |
| What is the study area in a scholarly peer-reviewed article? | it is where you are going to do the study and where in that area |
| What is a null hypothesis? | Ho= there will be no difference in the amount of algae in the Winthrop Lake and wetlands. |
| What is an alternative hypothesis? | Ha= > or < |
| Sections of a formal lab report | Citation, scientific title, author byline, abstract, introduction, materials and methods, Data collection, data analysis, Results, discussion, references |
| What can you find in the abstract? | it's a summary of the formal lab report |
| What can you find in the introduction? | a literature review, hypothesis, and rationale for hypothesis |
| What can you find in the results? | raw data, descriptive statistics(mean standard deviation), chi square or t-test results, and a narrative. |
| What can you find in the narrative? | an analysis of results, whether you accept or reject your hypothesis based on results, how your study compares to others, and what went wrong or right in your experiment. |
| What does scientific literature refer to? | it comprises scientific publications that report original empirical and theoretical work in the natural and social sciences. |
| what test is used to detect sugar? | Benedict's reagent |
| What test is used to detect starch? | Lugol's test |
| What test is used to detect lipids? | Sudan IV |
| What test is used to detect proteins? | Biuret reagent |
| What is a scanning electron microscope (SEM)? | Magnify very small specimens with very find detail. Useful to study outside of structure. Magnification from 10x-10000x or higher. Usually look like detailed 3 dimensional photos. It is an electron microscope. Good to look at the head of a bee or feather. |
| What is a transmission electron microscope? | Used to examine very thin slices of biological material. Useful for studying inside of structures. It is an electron microscope. Good for looking at ribosomes in a cell. |
| What is a fluorescent microscope? | Involves the use of fluorescent stains to visualize structures in an organism. It works by absorbing light and giving off light |
| What is a stereomicroscope? | Great for viewing opaque specimens. Provides a stereoscopic image. Can use to view coral, shells, penny, pond water, insects, or jewelry. It is a low-power microscope. |
| What is a compound light microscope? | You will use this microscope the most in this class. It has the three magnifying lenses. Good for looking at elodea plant cells or cheek cells. |
| What are the different units of measurement used in the lab quest probes? | temperature ( C ) , wind speed ( m/s ) , humidity ( % ) , soil moisture ( % ), dissolved oxygen ( mg/L ) , illuminance ( lux/m ) |
| what is eukaryotic cell? | Ex. Animal and plant cells. Contain a true nucleus and contain membrane-bound organelles |
| What is prokaryotic cell? | Ex. bacteria and archaeans. Single-celled organism that lacks a membrane-bound nucleus. |
| What is the difference in structure between a plant and animal cell? | Plant cells have additional structures; chloroplasts, the cell wall, and vacuoles. |
| What is resolving power? | the extent to which object detail in an image is preserved during the magnifying process |
| What is magnification? | the amount that the image of an object is enlarged |
| what are the three magnifying lenses of a compound microscope? | 4x , 10x , 40x |
| How do you calculate the total magnification? | (eyepiece) x (objective) ex: 10 x 40 =100 |
| What is contrast? | the degree to which image details stand out against their background. Fixing and staining. |
| What is the field of view? | It is the area that you see when looking into the microscope. It depends on the strength of magnification. The lower the power, the larger the point of view. |
| What is cytoplasmic streaming within Elodea cells? | It is the movement of cytoplasm within a cell. |
| What are fluorochromes used for in fluorescent microscopy? | they work by absorbing light and giving off light |
Created by:
taylors25