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Microscopy and Stain
Microscopy, Staining, and Classification Pt. 1
| Question | Answer |
|---|---|
| the distance between two corresponding parts of a wave. A. Magnification B. Wavelength C. Contrast D. Molecule | wavelength |
| the apparent increase in size of an object. Is indicated by a number and a x which reads in "times" A. Contrast B. Wavelength C. Microscope D. Magnification | magnification |
| the ability to distinguish objects that are close together A. Magnification B. Resolution C. Wavelength D. Compound | resolution "also called resolving power" |
| the use of light or electrons to magnify objects. A. Microscopy B. Wavelength C. Contrast D. Magnification | microscopy |
| Differences in intensity between two objects, or between an object and background. A. Wavelength B. Contract C. Wavelength D. Confocal | contrast |
| Important in determining resolution | contrast |
| increases the contrast of microorganisms and their background | staining(under contrast) |
| Use of light that is in _____________________ all the wave's crest and troughs are aligned can enhance the contrast. | phase(under contrast) |
| microscope in which the background or field is illuminated | Bright Field Microscope |
| microscope in which the specimen is made to appear light against a dark background | Dark Field Microscope |
| microscope that uses the alignment or misalignment of light waves to achieve the desired contrast between a living specimen and its background. | Phase Microscope |
| use individual ultraviolet light to cause specimens to radiate visible light | Fluorescent Microscopes |
| microscope that contains a single magnifying lens. A. Simple Microscope B. Compound Microscope C. Fluorescent Microscope D. Scanning Electron | Simple microscope |
| microscope more similar to magnifying glass than modern microscope | Simple Microscope |
| Examples of bright field microscope | Simple and Compound |
| Leeuwenhoek used this microscope to observe microorganisms | Simple Microscope |
| microscope that uses Series of lenses for magnification A. Compound B. Simple C. Confocal D. Fluorescent | Compound Microscope |
| microscope in which magnification allows light rays to pass through a specimen and into the objective lens | Compound Microscope |
| Oil immersion lens increases resolution. | Compound Microscope |
| determined by multiplying the magnification of the objective lens by the magnification of the ocular lens. | Total Magnification |
| microscope used to examine living microorganisms or specimens that would be damaged or altered by attaching them to slides or staining them. | Phase Microscope |
| Two types of Probe Microscope | Scanning tunneling Microscope and Atomic Force Microscope |
| Light rays are____________________ when their crest and troughs are aligned (brighter image) A. In Phase B. Out Phase | In Phase |
| Light rays are___________________________ when their crest and troughs are not aligned. (darker image) A. In Phase B. Out of Phase | Out of Phase |
| Microscope that Magnifies more than 100,000,000 times A. Probe B. Electron C. Light D. Transmission Electron | Probe Microscope |
| microscope that passes a metallic probe, sharpened to end in a single atom, back and forth across and slightly above the surface of a specimen. A. Scanning Tunneling B. Fluorescent C. Phase Contrast D. Differential Interference | Scanning tunneling microscopes |
| microscope that measures the flow of electrons to and from the probe and the specimen's surface. A. Atomic Force B. Transmission Electron C. Scanning Tunneling D. Confocal | Scanning tunneling microscope |
| amount of electron flow, that is directly proportional to the distance from the probe to the specimen's surface. A. Wavelength B. Scanning Tunneling C. Transmission D. Microscope | Tunneling Current(scanning tunneling microscope) |
| microscope that can measure distances as small as 0.01 nm and reveal details on the surface of a specimen at the atomic level. A. Atomic Force B. Scanning Tunneling C. Confocal D. Transmission Electron | Scanning tunneling microscope |
| microscope that must be electrically conductive A. Atomic Force Microscope B. Scanning Tunneling Microscope | Scanning tunneling microscope |
| microscope that uses a pointed probe but it traverses the tip of the probe lightly on the surface of the specimen rather than that of a distance A. Dark Field B. Atomic Force C. Scanning Electron D. Transmission Electron | Atomic force microscopes |
| microscope that can magnify specimens that do not conduct electrons. They can also magnify living specimens because and electron A. Atomic Force B. Phase Contrast C. Scanning Tunneling D. Transmission Electron | Atomic force microscope |
| researchers used this microscope to magnify the surfaces of bacteria, viruses, proteins and amino acids. A. Atomic Force B. Scanning Electron C. Scanning Tunneling D. Transmission Electron | Atomic force microscope |
| light microscope cannot resolve structures closer than 200 nm | Electron Microscope |
| types of electron microscopes | Transmission electron and scanning electron |
| microscope that has electron waves traveling between 0.01 nm and 0.001 nm which is 10, 000x to 100000x wavelength of visible light A. Light Microscope B. Electron Microscope C. Probe D. Phase | Electron Microscope |
| this microscope have greater resolving power and magnification than light microscopes. | Electron Microscope |
| provides detail view of bacteria, viruses, internal cellular structures, molecules and large atoms. | Electron Microscope |
| cellular structures that can be seen only using electron microscope are referred to as a cell's | ultrastructure |
| microscope that generates a beam of electrons that ultimately produces an image of a florescent screen. | Transmission electron microscope |
| uses magnetic fields within a vacuum to microscope that manipulate a beam of electrons called primary electrons. | Scanning Electron Microscope |
| rather than passing electrons through a specimen, this microscope focuses them back and forth across the specimen's surface. A. Transmission Electron B. Scanning Electron | Scanning Electron Microscope |
| produces three dimensional view of the surface of microbes and cellular surfaces. A. Scanning Electron B. Transmission Electron C. Probe D. Light Field | Scanning Electron Microscope |
| microscope Used to observe the surface details of structures | Scanning Electron Microscope |
| used to magnify up to 10,000x with resolution of about 20 nm. | Scanning Electron Microscope |
| specimens can be observed because sectioning is not required. A. Scanning Electron B. Differential Interference Contrast C. Transmission Electron D. Atomic Force | Scanning Electron Microscope |
| disadvantage of this microscope is that it magnifies only the external surface of a specimen and requires a vacuum, can only examine dead organisms. A. Scanning Electron B. Phase Microscope C. Probe Microscope D. Fluorescent Microscope | Scanning Electron Microscope |
| electrons pass through specimen through magnetic fields that manipulate and focus the beam, absorbs electrons change some energy into visible light A. Scanning Electron B. Confocal Microscope C. Transmission Electron D. Differential Interference Con | Transmission Electron Microscope |
| produces two dimensional images of ultrastructure of cells A. Transmission Electron B. Scanning Electron | Transmission Electron Microscope |
| microscope used to observe the internal ultrastructural detail of cells and observation of viruses and small bacteria A. Transmission Electron B. Atomic Force C. Scanning Electron D. Fluorescent | Transmission Electron Microscope |
| type of light microscopy | Confocal Microscope |
| type of light microscopy that uses ultraviolet lasers to illuminate florescent chemicals in a single plane of the specimen, no thicker than 1.0 um. A. Flourescent B. Confocal C. Phase D. Dark Field | Confocal Microscope |
| microscope when resolution increased because emitted light passes through pinhole aperture | Confocal Microscope |
| computer constructs 3-D image from digitized images A. Fluorescent B. Phase Contrast C. Confocal D. Dark Field | Confocal Microscope |
| direct uv light source at specimen A. Fluorescent B. Phase Contrast C. Dark Field D. Simple | Fluorescent Microscope |
| specimen radiates energy back as a longer visible wavelength A. Florescent B. Dark Field C. Phase D. Probe | Fluorescent Microscope |
| uv light increases resolution because it has shorter wavelength A. Atomic Force B. Phase Contrast C. Bright Field Microscope D. Fluorescent Microscope | Fluorescent Microscope |
| contrast improves because fluorescent structures are visible against a black background. A. Confocal Microscope B. Phase Microscope C. Fluorescent Microscope D. Scanning Microscope | Fluorescent Microscope |
| some cells are naturally fluorescent, others must be stained. | Fluorescent Microscope |
| used in immunofluorescence to identify pathogens and to make visible a variety of proteins | Fluorescent Microscope |
| used to examine living organisms or specimens that would be damaged/altered by attaching them to slides or staining A. Electron Microscope B. Confocal Microscope C. Fluorescent Microscope D. Phase Microscope | Phase Microscope |
| treat one set of light rays differently from another set of light rays A. Phase B. Dark Field C. Light Field D. Compound | Phase Microscope |
| two types of phase microscopes | Phase Contrast and Differential Interference Microscope |
| contrast is created because light waves are out oh phase | Phase Microscope |
| the simplest phase microscope, produces sharply defined images in which fine structures can be seen in living cells A. Phase Contrast B. Transmission Electron C. Scanning Electron D. Confocal Microscope | Phase-Contrast Microscope |
| type of phase microscope useful in observing cilia and flagella. A. Differential Interference Contrast B. Phase Contrast C. Dark Field Microscope D. Transmission Microscope | Phase Contrast Microscope |
| type of microscope also called Nomarski microscope, creates phase interference patterns | Differential interference contrast microscope |
| use prisms that split light beams into their component wavelengths(color)Increases contrast and gives the image a dramatic three dimensional or shadowed appearance, produces unnatural colors. | Differential interference contrast microscope |
| best for observing pale objects A. Bright Field B. Dark Field | dark field microscope |
| uses dark field stop in the condenser that prevents light from directly entering the objective lens. | dark field microscope |
| light rays are reflected inside the condenser so they pass the slide at oblique level. A. Dark Field Microscope B. Light Field Microscope C. Phase Microscope D. Probe Microscope | dark field microscope |
| only light rays scattered by specimen enter objective lens, increases contrast and enables observation of more details. Examine small and colorless cells. A. Dark Field B. Bright Field C. Electron D. Probe | dark field microscope |
| oil immersion lens increases resolution A. Simple B. Compound C. Fluorescent D. Confocal | compound |
| have one or two ocular lens A. Simple B. Dark Field C. Electron D. Compound | compound |
| most have condenser lens(direct light specimen) A. Simple Microscope B. Compound C. Differential Interference D. Scanning Electron | compound |
Created by:
Phole101