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Micro Lab Quiz 1
Micro
| Question | Answer |
|---|---|
| What are most microbes we use | BSL-1 (biosaftey level 1) |
| what is the safety for lab time | no food/drink, come to lab prepared, work carefully/ methodically-dont hurry, turn burner off when no in use |
| what is the aseptic part for lab time | wash hands before/after lab, wash desktop before/after lab, always lay tests tubes in holder, put all non-essential books away |
| what is the most common type of liquid media | NA broth (nutrient broth) |
| recipe for Nutrient broth | beef extract, peptone, distilled water |
| how do we know if a growth is present in a broth | liquid is cloudy (turbid), microbes collect on surface (pellicle) |
| what is the recipe for solid media | beef extract, peptones, 1-5% agar |
| why is solid media perfect for growing bacteria | solid at room temp, liquefies easily, does not degrade |
| what are three types of solid media | slant, deep, plate |
| slant | agar solidified at an angle--provides large surface area for growth |
| deep | good for anaerobic bacterial growth |
| plate | large surface area; great for aerobic bacteria |
| microbes can be | free living, associated with a host, reservoir |
| free living | do not reside on/ in a specific host, grow at room temp, typically non-pathogenic |
| associated with a host | grow at body temp, can be pathogenic |
| reservoir | any area that a microbe resides..desk/mouth |
| what was the purpose of incubating the unopened plates. What is an appropriate name for these plates? | sterile media/baseline comparison. Control |
| if growth appears on both unopened plates, what are some likely explanations? What if growth appears on only one plate? How does growth on the unopened plates affect the reliability of the other plates? | both- media was contemned before experiment one- more limey contamination during experiment. Less reliability=less confidence in results |
| why were the specific types of exposure chosen for this exercise? | bacteria grows everywhere, shows libquity. all exposures represent possible contamination during experiment. |
| why were you asked to incubate the plates at two different temps? What is the likely source of organisms that grew best at 37C and how do you think they survive at room temp without nutrients? | Capture ubiquity of microbes, some grow fast at room them others grow fast at body temp. Source for microbes at 37C= human body. survive due to: microbe is inert and nutrients present, but not visible. |
| the plates you are using for this lab will be autoclaved to completely sterilize them. The measures taken to disinfect the tabletops are not as extreme, why? | desktop- less nutrients, less density Petri dishes- high nutrients, high density |
| how is the glow germ hand wash defined | education system developed to train people to wash hands more effectively. Hand washing is the easiest, cheapest method of minimizing transfer to microbes |
| Glow germ...uniform compliance is difficult: | skin dryness, skin reactions to reagents, heavy workload/no time |
| alcohol-based hand rubs? | replaced conventional hand washing in many places- |
| why does alcohol based hand rubs appears to increase compliance by employees | more effective than soap, less time, produce fewer skin reactions, portability |
| what areas were cleaned most throughly with washing techniques | palms and back of hands |
| what areas were most difficult to clean with washing | fingernails, sides of hands, in-between fingers |
| were both of your hands cleaned at equal amounts? | non-dominate hand- cleaner? |
| shine the UV light on data sheet. how much lotion was transfer to the data sheet. What does this tell you about the was of transferring the unseen by contact? | easy to transfer germs |
| what do you use to sterilize tools/tubes | bunsen burner |
| inner cone and outer cone | inner- sterilizes instruments outer- heat fix slides/flame tubes |
| what is it called to sterilize instrument | flame the loop |
| once loop is sterilized do not? | wave it around, touch it, blow on it, lay on bench |
| what is it called to sterilize tubes | flame the tube |
| considering the cultures used to inoculate each medium, how many different microbial types should you expect | one- a successful aseptic transfer will have only micrococcus lutes growth- this would be a pure culture |
| streak method | allows for isolation of individual species from a mixed sample |
| is isolation an important 1st step in identification of a microbe | yes |
| what is an example of a mix culture | throat culture or urine sample |
| culture containing 2 or more species | mixed culture |
| how does a streak plate work | -streaking decreases cell density so individual cells drop off in last quadrant. -Individual cells will grow into pure colonies during incubation. -Follow on tests to ID a microbe can be run on pure colonies |
Created by:
Khuller