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Micros Test 1.2
| Question | Answer |
|---|---|
| 1 µm | 10^-6 m |
| 1nm | 10^-9 m |
| 1Å | 10^-10 m |
| 1 µm to nm to Å | 10^3 nm =10^4 Å |
| Most common microscope used for stained smears | compound light microscope |
| Microscope used for unstained bacterial cells when the cells are small and no detail is needed | dark-field microscope (shows a light silhouette of an organism against a dark background) |
| Microscope used for unstained live tissue where it is desirable to see some intracellular detail | phase-contrast microscope (uses a special condenser to enhance differences in the refractive indexes of cell's parts and its surroundings) |
| Microscope used for a sample that emits light when illuminated with uv light | fluorescent microscope(specimens first stained with fluorochromes, bright objects against dark backgrounds) |
| used with fluorescent-antibody technique, mainly for diagnosis, especially good for syphilis and rabies fluorescence microscopy | fluorescence microscopy |
| Microscope used for intracellular detail of a cell that is 1 micrometer long | Electron microscope (uses electrons instead of light, can view 3D views of surfaces of whole microorganisms) |
| Microscope used for unstained live cells in which intracellular structures are shown in color | Differential Interference Contrast (DIC) Microscope |
| How does an electron microscope differ from a light micrscope | uses a beam of electrons focused by magnets and the image is viewed on a television-like screen or photographic plate |
| What are the maximum magnification and resolution of a compound microscope | 2,000x and 0.2 micrometeres |
| What are the maximum magnification and resolution of an electron microscope | 100,000x and 0.0025 micrometers |
| One advantage to a scanning electron microscope over a transmission electron microscope | is the capacity of discerning depth in a 3D image |
| Acid dyes stain | the background in a smear |
| Acid dyes are used for | negative stains |
| Stain the cells in a smear | Basic stains |
| Basic dyes are used for | simple stains |
| Why do basic dyes stain bacterial cells | because bacterial cells carry a slight negative charge and the colored positive ion of a basic dye is attracted to the negative charge of the cell |
| Why do acid dyes not stain bacterial cells | because they are negatively charged |
| Simple stain | determines cell shape and arrangement |
| Differential stain | distinguishes kinds of bacteria based upon interaction of the staining procedure with a particular cell wall structure |
| Negative stain | does not distort the cell and is used to determine cell shape, size, and the presence of a capsule -dark background, colorless bacteria |
| Flagella stain | determines the number and arrangement of flagella uses a mordant and carbolfuchsin |
| Why is a mordant used in a gram stain? | because the mordant combines with the basic dye to form a complex that will not wash out of gram-positive cells |
| Why is a mordant used in a flagella stain? | because the mordant accumulates on the flagella so that they can be seen with a light microscope |
| What is the purpose of a counterstain in the acid-fast stain? | stains the colorless non-acid-fast cells so that they are easily seen through a microscope |
| What is the purpose of a decolorizer in the gram stain | it removes the the stain from the gram-negative cells |
| What is the purpose of a decolorizer in the acid-fast stain? | it removes the stain from the non-acid-fast stain |
| What is the counterstain in the edospore stain | Safranin |
| What is the counterstain in the gram stain | safranin |
| In gram staining, after the crystal violet gram positive cells are | purple |
| In gram staining, after the iodine gram positive cells are | purple |
| In gram staining, after the alcohol-acetone gram positive cells are | purple |
| In gram staining, after the safranin gram positive cells are | purple |
| In gram staining, after the crystal violet gram negative cells are | purple |
| In gram staining, after the iodine gram negative cells are | purple |
| In gram staining, after the alcohol-acetone gram negative cells are | colorless |
| In gram staining, after the safranin gram negative cells are | red |
| What is the order of the gram staining | crystal violet, iodine, alcohol-acetone, safranin |
Created by:
hweems90