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Micro test 1 study

Study for MIP 300 test 1

QuestionAnswer
Bright Field Microscope Must heat-fix and stain bacteria. Kills bacteria
Dark Field Microscope Useful for visualising live specimen that are delicate like spirochetes.
Treponema pallidum ----------------- A spirochete bacterium that can be seen only with dark field microscopy. Causes syphilis
Phase Contrast Microscopy Used for live samples. allows the determination of depth easily.
Flourescent Microscopy Uses UV light to excite proteins and labeled antibodies. Can be used to see Streptococcus pyogenes.
Electron Microscope Most powerful of the microscopes. All specimens must be killed.
Gram Stain Differentiate gram + and - bacteria. Crystal violet, grams iodine, decolorizer, safranin.
Acid fast stain Identify bacteria with mycolic acid coat. Acid fuchin and heat, acid alcohol wash, methylene blue.
Endospore stain Used to identify spores and endospores. Green and heat, water rinse, safranin.
Coccus Round. Diplo... Pairs, Strepto... Chains, Staphylo... Clusters, Tetrads 4 cell groups, Sarcina 3 planes 8 cell groups
Bacillus Rod. Diplo... Pairs, Strepto... Chains, Coccobacillus short and wide
Vibrios Curved rods. eg Vibrio cholera
Spirals Spirochete, Spirilla have flagella
Mycoplasm Extremely variable shape with no cell wall. Mycoplasm pneumonia causes walking pneumonia.
Archaeal cell walls larger variety with pseudopeptidoglycan, many s layers that protect the cells.
Bacterial Cytoskeleton Contains homologues of tubulin and actin. Functions in cell shape, localizing protein, and aids in cell division.
Glycogen granules inclusion bodies in bacteria that store glycogen
Cyanophycin granules inclusion bodies in bacteria that store amino acids and nitrogen
Gas vacuole inclusion bodies in bacteria that store air allowing the bacteria to float. they are made up of protein
Metachromatic granules inclusion bodies in bacteria that store phosphate
Magnetasomes inclusion bodies in bacteria that store iron
Bacterial spore structure 15% calcium dipicolinic acid, ribosomes, nucleic acids, surrounded by thick peptidoglycan layer (cortex), many layers of protein (spore coats)
Created by: 100000323760050
 

 



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