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genetics test 2

QuestionAnswer
crossing over during prophase I of meiosis occurs between alleles of sister chromatids false
Parental gametes can be formed if there is no crossing over during meiosis false
If two genes on the same chromosome exhibit complete linkage what is the expected F2 phenotypic ratio from a self heterozygote with the genotype a+b+/ab 3:1
In a two point test cross involving unlinked genes what phenotypic ratios would be expected from a cross between dihybrid female and a homozygous recessive male? 1:1:1:1
who is created with creating the first linkage map alfred sturtevant
What is the definition of a map unit one percent recombination
Syntetic genes can assort independently when they are far apart on the chromosome and crossing over occurs frequently between the genes
The alleles of linked genes tend to segregate together during gamete formation
the role of transposase activity in IS element transposition includes cutting DNA at the target sequence
what is the name of the process in bacteria that involves DNA transfer through a cytoplasmic connection known as a pilus conjugation
what is the name of the complex that binds to the origin of transfer in the process of conjugation relaxosome
how is an Hfr chromosome formed by integration of the F plasmid into bacterial chromosome
Why does conjugation with an Hfr donor result in a much higher rate of gene transfer than conjugation with an F+ donor The Hfr donor transfers genes from the chromosome not the plasmid
A large region of the F plasmid is called the transfer region (tra) and contains about 40 genes necessary for conjugation. The gene traA codes for the protein pilin What other functions of the tra genes are required for transfer? DNa unwinding and restriction of contact with the F+ cells
When F+ conjugation occurs successfully, only one copy of the F plasmid single-stranded DNA is transferred. What else might minimally occur? stopping the rolling circle replication after the copy of one length, circulization, and replication
What conjugation type is a stable partial diploid F'
An F donor includes F DNA plus a segment of bacterial chromosome DNA. If conjugation is interrupted before the entire F' chromosome transfer what could result? failrue of the F strand to circulize in the recipient
In which process of genetic exchange in bacteria is one strand of DNA degraded as it enters the recipient Transformation
What is the name of a bacteriophage genome integrated into a host cell chromosome prophage
Which process of genetic exchange in bacteria is capable of transferring only a small specific region of the bacterial chromosome specialized transduction
In generalized transduction a phage introduces a segment of donor DNA into the recipient cell This is followed by recombination of the donor fragment with the recipient chromosome. What must occur a pair/even number of crossovers between donor segment and recipient
Some bacteria phages can be either lytic or lysogenic. These are called_____ phages temperate
Events involved in transition transfer integration precision excision
capable of transferring largest chromosome segment from donor to recipient conjugation
transfers smallest donor segments to recipients transduction
Guanine and Adenine are both purines found in DNA True
ddNTPs have a hydrogen at the 3' carbon of the sugar True
DNA fragments that are 600bp long will migrate more quickly through a sequencing gel than 150 bp False
In manual sanger sequencing the DNA sequencing obtained is complementary to the template strand true
The thermostability of taq polymerase is required during the annealing phase of PCR False
what is the purpose of raising the temperature 90-95C at the beginning of each cycle inPCR to separate the double stranded DNA
For polarity of DNA strands the 3' end has a free OH group true
To help achieve proper base pairing and hence form a double helix what must be met a purine base must be paired with pyrimidine base
the data obtained from the meselson-stahl experiment after one generation of replication eliminated the dispersive model of DNA replication false
which enzyme catalyzes the addition of nucleotides to a growing DNA chain DNA polymerase
single-strand binding proteins stabilize the open conformation of the unwound DNA True
In 1928, Frederick Griffith conducted a series of experiments that demonstrated which of the following? A "transformation factor" could be released from one cell and picked up by another cell, thus changing the genetic identity of the recipient cell.
In 1944, Avery, Macleod, and McCarty provided direct evidence that DNA was the hereditary molecule. Ttracts from lethal SIII bacteria with various enzymes and found that transformation was prevented only upon treatment with _______. DNase
In a DNA nucleotide, to which carbon on deoxyribose is the base attached? 3'
Which of the following forms the backbone of a polynucleotide chain? alternating sugar and phosphate
Which of the following statements about DNA replication in E. coli is true? Replication proceeds in both directions from a single origin of replication.
What is the maximum rate of DNA replication in E. coli (e.g., the rate at which DNA polymerase incorporates new nucleotides into a polynucleotide chain)? 1000 nucleotides per second
Which protein is responsible for initial denaturation of oriC in E. coli? dnaA
Which enzyme is responsible for relieving the torsional stress caused by DNA unwinding in the process of DNA replication? Topoisomerase
Which enzyme in E. coli is responsible for the removal of RNA primers from DNA? DNA polymerase I
Which of the following types of cells typically displays little or no telomerase activity? Differentient somataic cells
Which of the following reagents is not required for the polymerase chain reaction? Dideoxynucleotide triphosphates
Created by: kristen0822
 

 



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