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Micro/Lab004
Endospore, Capsule, Flagella
Question | Answer |
---|---|
endospore stain without heat, true or false | true |
what are the gram reactions of clostridium & bacillus | both are gram positive |
how might a capsule, & flagella contribute to an organism pathogenicity | capsules prevent phagocytosis, & allows attachment prior to infection. Flagella moves organism towards host |
what advantage of clostridium is an endospore | endospores allow survival from aerobic conditions |
You can see endospores by simple staining. Why not use this technique? | endospores are visible in simple stains, however the clear areas seen could be caused by other structures such as storage granules. |
how would an endospore stain of mycobacterium appear? | mycobacterium would probably stain green because of the thick cell wall |
what type of culture medium would increase the size of a bacterial capsule | the size of bacterial capsule composed of polysaccharides would be increased by a culture medium rich in disaccharides and polysaccharides |
Describe the microscopic appearance of encapsulated Streptococcus if stained with safranin and nigrosin | The stretococcal cells would be red surrounded by a clear capsule against a black background |
In the Dorner endospore stain, a smear with carbolfuchsin is steamed, then decolorized with acid alcohol and counterstained with nigrosin. Describe the microscopic appearance after this procedure. | The endospores will be red in a colorless cell background will be black |