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Micro/Lab003

Acid-Fast Staining

QuestionAnswer
whats the purpose of acid fast staining? To detect cells capable of retaining a primary stain
what are the large blue stained areas on a sputum slide fragmented white blood cells
what is the decolorizing agent in gram stain ethyl alcohol, or ethyl alcohol acetone, or acetone
what is the decolorizing agent in acid fast stain acid alcohol
what diseases are diagnosed using acid fast tuberculosis, leprosy, nocardiosis, acitinomycete, & cryptospordium infections
what is phenol and what is its usual application phenol (carbolic acid) is a disinfectant, it was a disinfected used by lister
how might the acid fast characteristic of mycobacterium enhance the organisms ability to cause a disease? Cell wall includes lipids resist; drying, stress, harsh conditions, & phagocytes
clinical specimens suspected of containing mycobacterium are digested with sodium hydroxide for 30 mins prior to staining why is this used, why isnt this used for other bacteria digestion removes:sputum, & human cells, others would be killed
the acid fast stain is used to detect cryptospordium protozoa in fecal samples, which would you expect to be a major compotent of their cell wall lipid, carbohydrates, or proteins? what disease is caused by crytospordium? lipids, cryptosporidiosis diarrheal disease
in 1882 after experimenting with staining mycobacterium, paul ehrlich wrote that only alkaline disinfectants would be effective against mycobacterium, how did he reach this conclusion without disinfectants mycobacterium are resistant to acid
Created by: eve amaya
 

 



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