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Micro/Lab0001
Negative Staining
| Question | Answer |
|---|---|
| Why is the size more accurate in negative stain than in direct stain | negative stain is more accurate than positive stain because no heat is involved & stain does not penetrate the cell. However some shrinkage does occur |
| Could any dye be used in place of nigrosin for negative staining? what types can be used | No only a cell that does not penetrate the cell, india ink works |
| why were u instucted to discard the toothpick disinfectant | safe disposal to preventing spread of infectious material |
| what microscope technique gives a field similar in appearance of a negative stain | dark microscopy |
| carbolfuchsin can be used as a direct stain and as a negative stain | as a direct stain, ph is acidic |
| acidic congo red does not contain carbon particles like nigrosin but can give the appearance of a negative stain. What is the basis for this stain? | the large carbon particles cannot enter the cell so the cell remains colorless against a dark background |