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Enterics
| Question | Answer |
|---|---|
| E. coli | Indole pos MR pos VP neg Citrate neg |
| Proteus mirabilis | Indole neg MR pos VP variable Citrate varibale |
| Edwardsiella tarda | Indole pos MR pos VP neg Citrate neg |
| Citrobacter koseri | Indole pos MR pos VP neg Citrate pos |
| Klebiella pneumonia | Indole neg MR neg VP pos Citrate pos |
| Salmonella typhi | Indole neg MR pos VP neg Citrate neg |
| Serratia marcescens | Indole neg Mr neg VP pos Citrate pos |
| Proteus vulgaris | Indole pos MR pos VP neg Citrate neg |
| Enterobacter aerogenes | Indole neg MR neg VP pos Citrate pos |
| Providencia stuartii | Indole pos MR pos VP neg Citrate pos |
| Klesiella oxytoca | Indole pos MR neg VP pos Citrate pos |
| Shigella sonnei | Indole neg MR pos VP neg Citrate neg |
| Aeromoan spp. can resemble lactose fermenting normal stool flora on SBA and MAC. What 2 rapid screening tests can be performed on suspected Aeromonas colonies | Oxidase and spot indole |
| How is a Helicobacter pylori infection typically diagnosed | Performing rapid urease test on a gastric biopsy specimen |
| What cultre condition provides optimal growth and isolation of Yersinia enterocolitica? | Cold enrichment or lower temp 25-30°C |
| What serogroup is Shigella sonnei | serogroup D |
| What serogroup is Shigella boydii | serogroup C |
| What serogroup is Shigella flexneri | serogroup B |
| What serogroup is Shigella dysenteriae | A |
| What strain of E. coli is referred to as Enterohemorrhagic E. coli | E coli 0157:H7 |
| What are the primay virulence factor of E. Coli 0157:H7 | 2 cytotoxins, Verotoxin I and II |
| How does Enteroinvasive E. coli (EICE) produce dysentery | Direct penetration invasion, destruction of intestinal mucosa |
| What genrea of Enterics are PAD positive | Proteus, Morganella, Providencia |
| What amino acids are used in Decarboxylase and Dihydrolase test and what do they produce? | Kysie, ornithine, arginine Amines |
| Why does XLD agar contain sodium thiosulfate and ferric ammonium citrate? | for H2S production |
| Why does HE agar contain bile salts | to inhibit gram positives |
| the spot indole procedure test for which bacterial enzyme? | tryptophanase |
| The onpg assay test for which bacterial enzyme | beta-galacosidase |
| what is the reagent used in the phenylalanine deaminase test? | 10% ferric chloride |
| what 2 reagents are aused in the vp test | alpha-naphthol and 40% KOH/NaOH |
| Organisms in the Proteus, Morganella and Providencia groups can be separated from the other Enteric organism groups because they are positive for what test> | Deaminase or Phenylalanin deaminase (PAD or PD) |
| What test can aid in separating Klebsiella pneumonia from many of the Enterobacters? | Ornithin decarboxylase is promary also Arginine dihydrolse. |
| What simple test can separate E cole from omst of the Cirtobacter, Enterbacters, and Klebsiellas | Citrate |
| What are the three carbohydrates in TSI | 0.1% Glucose, 1% lactose, and 1% sucrose |
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