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Gram Negative Cocci
Gram Negative Cocci--Neisseria and Moraxella
| Question | Answer |
|---|---|
| What is the gram stain reaction for Neisseria and Moraxella? | gram negative diplococci (kidney bean shape) |
| What is the oxidase reaction for Neisseria and Moraxella? | oxidase positive (purple color) |
| What media does Neisseria grow on? | chocolate agar, Modified Thayer Martin (contains vancomycin, colistin, nystatin)--usually doesn't grow on blood agar NEVER REFRIGERATE THE SPECIMEN |
| What atmosphere does Neisseria require? | Neisseria are capnophiles--need increased CO2 |
| What tests are positive for Neisseria gonorrhea? | N. gonorrhea ferments glucose (only)and is oxidase positive and catalase positive; is PRO positive |
| What tests are positive for Neisseria meningitidis? | N. meningitidis ferments glucose and maltose; is oxidase and catalase positive; is GGA positive and may be PRO positive |
| What tests are positive for Moraxella catarrhalis? | Moraxella doesn't ferments sugars; is oxidase and catalase positive; is butyrate esterase positive (IB); is DNase positive |
| What diseases does Neisseria gonorrhea cause? | gonorrhea, sexually transmitted disease, eye infections in babies (prevented by putting antibiotic drops in babies eyes); disseminated gonococcal infection, PID |
| What diseases does Neisseria meningitis cause? | normal flora of upper respiratory tract, people maybe carriers; meningitis especially in college age group and military recruits, there is vaccination for group A, C, Y and W135; fulminant meningococcemia--and DIC |
| What diseases does Moraxella catarrhalis cause? | it may be part of normal flora in nasopharynx; it is associated with respiratory tract infections including bronchitis and pneumonia in older and immunocompromised adults, and otitis media and sinusitis in children. |