D Bac Exam 2 Word Scramble
|
Embed Code - If you would like this activity on your web page, copy the script below and paste it into your web page.
Normal Size Small Size show me how
Normal Size Small Size show me how
Question | Answer |
Enterobacteriaceae | gram postive rods, non spore forming, grow on peptone media, grow on macconkey agar, usually faculatative anaerobes, ferment glucose with gas production, catalase +, oxidase -, reduce nitrate. |
Friedlander's Bacillus ("brick red" sputum) | K. pneumoniae |
endotoxin shock (symptoms) | fever, leucopenia, capilary hemorrhage, hypotension, circulatory collapse |
limulus lysate assay | reagent prepared from the ameboctytes of the horseshoe crab (Limulus polyphemus). Tests for endotoxin shock |
lipid A | toxic portion of lipopolysaccharide of gram negative cell wall that produces endotoxin |
Flagellar antigen | H antigen |
Capsular antigen | K antigen |
Somatic antigen | O antigen |
Ewig | developed the concept of tribes with Enterobacteriaceae |
tribe I | Eschericheae |
tribe II | Edwardsielleae |
tribe III | Salmonelleae |
tribe IV | Citrobactereae |
tribe V | Klebsielleae |
tribe VI | Proteeae |
tribe VII | Yersinieae |
Methyl Red Test | tests for mixed acid fermentation (red below pH 4.4 and yellow above pH 6.2) |
Voges Proskauer Test | tests for butanediol fermentation |
Embden-Meyerhof pathway | series of glycolytic cleavages that result in the production of pyruvic acid from glucose |
ONPG-galactose test | tests for beta galactosidase and beta galactoside permease, (+ = yellow, - = colorless) |
enzyme needed for fermentation of lactose | beta galactoside permease and beta galactosidase |
Kligler Iron Agar (KIA) | contains lactose and glucose (10:1), ferrous sulfate, and phenol red |
KIA: alkaline slant (red)/ alkaline butt(red) | no carbohydrate fermentation |
KIA: alkaline slant (red)/ acid butt (yellow) | glucose fermented but lactose not (ex. Shigella) |
KIA: alkaline slant (red)/ acid butt (black) | glucose fermented but lactose not, production of hydrogen sulfide (Salmonella, Citrobacter, and Proteus) |
KIA: acid slant (yellow)/ acid butt (yellow) | glucose and lactose fermented (ex. E. coli, Klebsiella, and Enterobactor) |
MacConkey Agar | differential media for selection and recovery of Enterobacteriaceae |
MacConkey Agar: red colonies | strong lactose fermenters (ex. Escherichia, Klebsiella, Enterobactor) |
MacConkey Agar: pink colonies | weak lactose fermenters (ex. Citrobactor, Providencia, Serratia, and Hafnia) |
MacConkey Agar: colorless colonies | Non lactose fermenters (ex. Proteus, Edwardsiella, Salmonella, Shigella) |
Eosin Methylene Blue Agar | differentiates between lactose and non lactose fermenters (similar to MacConkey Agar in the detection and differentiation of Enterobacteriaceae) |
Eosin Methylene Blue Agar: green black colonies with metallic sheen | Strong lactose fermenters (E. coli) |
Eosin Methylene Blue Agar: purple colonies | weak lactose fermenters |
eosin methylene Blue Agar: purple to black colonies | sucrose fermenters and non lactose fermenters (ex. Yersinia enterocolitica) |
Hektoen Agar | highly selective for recovery for Enterocacteriaceae (green media). Good for enhanced recovery of Salmonella and Shigella from material with heavy numbers of normal flora. |
Hektoen Agar: bright orange, pink | rapid lactose fermenters (E. coli) |
Hektoen Agar: blue green colonies with black centers | Salmonella |
Hektoen Agar: more green colonies | Shigella |
Xylose lysine deoxycholate agar | highly selective media for isolation of enterics, less inhibitory than HE agar (low conc. of bile salts), designed to detect shigellae in feces after enrichment in gram-negative broth. |
Selenite Broth | used for recovery of Salmonella and Shigella from feces or sewage. maintain predominant E. coli in lag phase |
ingredient in Selenite broth that inhibits E. coli | sodium selenite |
indole | produced by the breakdown of trptophan, also produce is pyruvate and ammonia |
tryptophanase | enzyme that breaksdown tryptophan |
Kovac's reagent | p-dimethylaminobenxaldehyde, used in the detection of indole (red = +) |
Citrate utiliatization | tests ability of strain to utilize citrate as a sole carbon and energy source |
Citrate utilization: positive result | blue color |
E. coli | indole +, methyl red +, VP -, Citrate - |
Enterobacter aerogenes | indole -, methyl red -, VP +, citrate + |
Created by:
rburt
Popular Bacteriology sets