Microbio 1 Final
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| sterile | free of microorganisms
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| inoculum | a culture medium in which microorganisms are implanted
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| culture | microorganisms that grow & multiply in a container of culture medium
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| colony | a visible mass of microbial cells arising from 1 cell or from a group of the same microbes
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| sterilization | the removal of all microorganisms, including endospores
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| disinfection | Process used to destroy microorganisms; destroys all pathogenic organisms except spores
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| sepsis | the presence of pus-forming bacteria or their toxins in the blood or tissues
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| sanitation | maintaining a clean condition in order to promote hygiene and prevent disease
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| aseptic | free from disease-causing microorganism
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| antiseptic | a substance that destroys micro-organisms that carry disease without harming body tissues
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| -cide | to kill
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| rate of microbial death factors | # of orgs: how long it takes to reduce the # of orgs to a given level
environmental influences: heat lowers pH, org matter may interfere
time of exposure: longer/shorter exposures to heat
characteristics: diff susceptiilities to physical & chem contro
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| how do microbial control agents alterate membrane permeability? | alterations: susceptibility of the plasma membrane is due to its lipids & protein components; certain chemical control agents damage plasma membrane
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| how do microbial control agents damage proteins/nucleic acids? | damages: some microbial control agents damage cellular proteins by breaking H+ bonds & covalent bonds; other agents interfere with DNA & RNA replication & protein synthesis
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| symbiosis | the relation between two different species of organisms that are interdependent
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| types of symbiosis | commensalism: 1 organism benefits, the other is unaffected
mutualism: both organisms benefit
parasitism: 1 organism benefits by deriving nutrients at the expense of the other
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| nosocomial infections | Infections acquired in a hospital or other healthcare setting.
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| Koch's postulates | 1) Pathogen must be present in all disease cases
2) Isolate pathogen, cultivate in pure culture
3) Inoculate into susceptible animal, initiate disease symptoms
4) Re-isolate pathogen, confirm it's the same pathogen
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| how did koch's work contribute to the study of microbio? | provided a framework for the study of the etiology of any infectious disease
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| symptoms | changes in body function
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| signs | seen heard measured or felt
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| syndrome | a group of symptoms or signs that collectively characterize or indicate a disease, disorder, abnormality, etc.
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| epidemic | short term
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| incidence | # of people in a population who develop disease in a particular time period
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| pandemic | worldwide
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| endemic | constantly present
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| latent | inactive for a while then resents signs/symptoms
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| 3 portals of entry | Mucous Membranes, skin, & Parenteral
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| diseases caused by exotoxins | diptheria, botulism, tetanus, cholera, food poisoning, TSS
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| how does antigenic variation benefit a pathogen? | by the time the body mounts an immune response against a pathogen, the pathogen has already altered its antigens & is unaffected by antibodies
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| herd immunity | individuals of a population will be protected from a disease to prevent its rapid spread to those in the population who are not vaccinated; many immune people present in a community
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| morbidity | the relative incidence of a particular disease
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| mortality | the ratio of deaths in an area to the population of that area
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| what factors contribute to the emergence of new infectious diseases? | new strains, global warming/weather pattern changes, widespread use of antibiotics/pesticides, known diseases spread to different regions, animal control measures, unrecognized infections in regions undergoing ecological changes by natural disaster, wars
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| why do certain diseases have to be reported to the US public health service? | provides epidemiologists with an approximation of incidence & prevalence of a disease, info helps officials decide whether or not to investigate, possible to control measures to stop disease from spreading
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| optimal pH for most bacterial growth? | 6.5-7.5
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| how can high osmotic pressure be used to control microbial growth? | high concentrations of salt or sugar inhibit microbial growth, loss of water from microbe inhibits metabolic functions of cell
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| biofilms | Surface coating colonies which secrete signalling molecules that recruit nearby cells causing the colony to grow. These cells also produce proteins that adhere to substrate and allow nutrients to reach the center and for wastes to be expelled.
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| culture medium | nutrients prepared for microbial growth
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| how many ATP are produced for each NADH mol? | 1 ATP = 6 NADH
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| how many ATP are produced for each FADH mol? | 1 ATP = 2 FADH
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| fermentation | a process in which an agent causes an organic substance to break down into simpler substances
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| lactic acid fermentation | The conversion of pyruvate to lactate with no release of carbon dioxide; bacteria only
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| alcohol fermentation | Glycolysis followed by the conversion of pyruvate to carbon dioxide and ethyl alcohol.
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| Linnaeus | binomial system of nomenclature
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| hooke | corks; 1st to document seeing cell in microscope
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| leeuwenhoek | made microscopes as hobby, using his own bodily fluids as slides
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| virchow | biogenesis & cell theory
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| pasteur | rabies vaccine, proved biogenesis
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| semmelweis | handwashing
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| lister | sterilization in OR
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| koch | tb
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| jenner | smallpox vaccine
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| ehrlich | blood/brain barrier
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| flemming | penicillin
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| golden age of microbio | fermentation, germ theory of disease, vaccinations
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| domains | bacteria: animals, plants, fungi, protists
archaea: prokaryotes that lack peptidolglycan cell walls
eukarya: all pathogenic & many nonpathogenic prokaryotes
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| endosymbiotic theory of origin of eukaryotes | larger bacterial cells that lost theis cell walls & engulfed smaller bacteria cells, 1st euk cells evolved from prok cells, proks & euks differ mainly in that euks contain highly specific organelles
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| what evidence suggests that mitochondria & chloroplasts were once free-living bacteria? | their ribosomes resemble that of proks & their mechanism of protein syn is more similar to that found in bacteria than euks
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| prokaryotic species | A population of cells with similar characteristics
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| viral species | a group of viruses sharing the same genetic information and ecological niche
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| culture | bacteria grown at a given time in media
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| clone | pure culture, population of cells derived form a single parent cell
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| strain | pure cultures of same species are not identical in all ways
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| glycocalyx | a capsule made up of a fuzzy coat of sticky sugars
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| capsule | a sticky, gelatinous substance around the cell wall; allows cells to stick together or to the host cell
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| slime coat | loose and unorganized glycocalyx
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| EPS | helps cells in biofilms attach to target enviros & each other
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| gram + | less lipids in their cell wall, thick pglycan wall, 2 inner rings
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| Gram - |
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| 4 essential elements that make up biological organisms | carbon, oxygen, nitrogen, hydrogen
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| ionic bond | the attraction between oppositely charged ions
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| covalent bond | a bond formed when atoms share one or more pairs of electrons
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| hydrogen bond | Weak attraction between a hydrogen atom and another atom
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| synthesis reaction | a reaction in which two or more substances combine to form a new compound
A+B=AB
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| decomposition reaction | break into smaller parts
AB=A+B
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| exchange reaction | part synthesis, part decomp
AB+CD=AD+BC
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| organic compounds | contain carbon & hydrogen, structurally complex
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| inorganic compounds | smally & simple, lack carbon
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| hydrolysis | a chemical reaction in which water reacts with a compound to produce other compounds
H+ & OH- of water mol
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| dehydration synthesis | process where water is removed to put 2 smaller molecules together
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| acid | compound that forms hydrogen ions (H+) in solution
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| base | any substance that forms hydroxide ions (OH-) in water
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| salt | dissolves in water to cations & anions, neither of which is H+ or OH-
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| oxidation-reduction reaction | any chemical change in which one species is oxidized (loses electrons) and another species is reduced (gains electrons)
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| cellular respiration | process that releases energy by breaking down glucose and other food molecules in the presence of oxygen
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| aerobic respiration | cellular respiration that uses oxygen, sequentially releasing energy and storing it in ATP
final e- acceptor: O2
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| anaerobic respiration | Respiration in the absence of oxygen. This produces lactic acid.
final e- acceptor: NOT O2
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| glycolysis | a metabolic process that breaks down carbohydrates and sugars through a series of reactions to either pyruvic acid or lactic acid and release energy for the body in the form of ATP
net gain of 2 mols/1 mol glucose
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| products of Kreb's cycle | 3 nadh, 1 fadh2, 2 carbon dioxide, atp
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| electron transport chain | A sequence of electron carrier molecules (membrane proteins) that shuttle electrons during the redox reactions that release energy used to make ATP.
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| chemiosmotic model of ATP generation | proton pumps pump electrons out of hte membrane to produce electrochemical gradient. Then diffuses across using ATP synthase, synthesizing ATP from ADP+Pi= 34 ATP
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