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microbiology

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Question
Answer
The study of what is too small to be seen with the naked eye   Microbiology  
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100 B.C. first to suggest that microbes exist, and may cause disease   Lucretius  
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1673 crude microscope 300x, "microbes are everywhere"   Antony Vanleeuwenhoek  
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300 B.C. supported the concept of spontaneous generation, living things can develop from non living matter   Aristotle  
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1668 First person to challenge the concept of spontaneous generation, observation of maggots on meat.   Francesco Redi  
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living things can only arise from pre-existing living cells   Biogenesis  
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1673 said "microbes must originate from spontaneous generation"   Van Leeuwenhoek  
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1745 Challenged spontaneous generation for microbes, observation of beef broth that turned cloudy.   John Needham  
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1765 Improved Needhams experiment, with a conclusion of biogenesis although people did not believe him   Lazzaro Spallanzani  
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1861 Improved Spallanzani's experiment, creator of the swan neked flask, conclusion biogenesis   Louis Pasteur  
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1860 First indirect evidence that microbes may cause disease, observation of post surgical disease   Joseph Lister  
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1876 First direct evidence that microbes cause disease   Robert Koch  
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Showed that the bacterium, bacillus anthraces, caused the disease anthrax   Joseph Lister  
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A set of rules/criteria for establishing a link between a disease and specific microbe   Koch's Postulates  
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1796 Creator of the first known vaccination, observation of milkmaids and smallpox   Edward Jenner  
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1880 studied cholera in chickens, concluded exposure to some disease provided protection against the fresh and strong bacteria   Pasteur and Chamberland  
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Coined the term vaccination in honor of jenner's work   Louis Pasteur  
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1906 developed a drug to treat syphilis called "salvarsan" a derivative of arsenic   Paul Ehrlich  
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1928 Studied staph and accidentally discovered penicillin   Alexander Fleming  
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Most microbes are classified as either   Prokaryotes or Eukaryotes  
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Before the kernel, lacks a true nucleus   Prokaryotes  
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True kernel has a true nucleus   Eukaryotes  
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Bacteria   Prokaryotes and no true nucleus  
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Fungi, algae, and protozoans   Eukaryotes and no true nucleus  
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Prokaryotic cell size   0.2-2.0 nm in diameter  
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Cell shape Coccus   roughly spherical  
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Cell shape Bacillus   rod shaped  
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Cell shape Spirilla   rigid spirals  
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Cell shape Spirochetes   flexible spirals  
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Cell shape Vibrio   curved rod or bent  
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Cell shape Fusiform bacillus   rod shaped with tapered ends  
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Cell shape Coccobacillus   elongated spheres  
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cell arrangement Singles   cell separate and do not remain attached  
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cell arrangement Pairs   diplococcus  
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cell arrangement Chain   streptococcus  
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cell arrangement Packets of 4 cells   tetrods  
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cell arrangement Packets of 8,16, 32 cells in cubes   sarcinae  
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cell arrangement Random Clusters   Staphylococcus  
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cell arrangement Step like arrangement   palisades  
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colony characteristics are determined by   cell shape  
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Present in all prokaryotic cells that separates interior of the cells from exterior of the cells   plasma membrane  
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2 basic components to plasma membrane   phospholipid bilayer, and proteins associated with bilayer  
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Phospholipid head   polar/hydrophilic  
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Fatty acid tails   non polar/hydrophobic  
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Functions of Plasma Membrane   site of metabolic reactions such as cellular resppiration,sence/gather information, and selectively permeable membrane  
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Passive Diffusion   movement from higher concentration (no energy required) to lower concentration, rate depends on concentration gradient.  
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Facilitated Diffusion   movement from higher concentration to lower concentration  
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Active transport   Movement from lower to higher concentration  
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An additional layer outside/exterior to the plasma membrane   cell wall  
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Cell wall   Functions are for protection, determines and maintains cell shape  
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Composed of many layers thick layers of peptidoglycan anchored by teichoic acid   Gram +  
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Composed of a relatively thin layer of peptidoglycan and an outer membrane   Gram -  
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Cells are less permeable more difficult for PCN to enter   Gram -  
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sugar shell, an additional layer outside/exterior to the cell wall not present in all prokaryotes, allows cell to attach/adhere to solid surfaces, protects cell from drying out, prevents cells from being engulfed by the immune system. Mostly polysacs   glycocalyx  
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Types of glycocalyx   Capsule= relatively thick, well organized and firmly attached to cell wall, with capsule comes disease. Slime layer= thin,not well organized and loosely attached to cell wall, typically attach to teeth.  
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Relatively short hair like extensions from the cell surface composed of a protein called pilin   Pili and Fimbriae  
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Pili   longer than fimbriae but less numerous  
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Fimbrae   shorter than pili, but more numerous  
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Relatively long hair like extensions from cell surface, usually many times the cell length   Flagella used for motility  
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Flagella   Basal body, hook, and filament  
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Motion of prokaryotic flagellum is by   rotation  
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Counterclockwise rotation of flagellum   propels cell forward "Run"  
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Clockwise rotation of flagellum   causes cell to stop or "tumble"  
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Average speed of flagellum   2-10 body lengths per second  
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Flagellar arrangements Monotrichous   one flagellum  
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Flagellar arrangements Amphitrichous   one or more flagellum at both ends  
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Flagellar arrangements Lophotrichous   two or more flagella at one end  
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Flagellar arrangement Peritrichous   Many flagella all around  
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A mini dormant version of the cell, very resistant to harsh environments   Endospore  
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Fluid inside the cell composed primarily of water and also contains proteins, carbs, nucleic acid, salts, vitamins etc.   Cytoplasm  
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A structure composed of DNA usually a single circular DNA molecule that may contain up to 3500 genes, stores genetic info.   Nucleoid  
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Usually a small circular fragment of DNA containing 5-100 genes, instructions for protein synthesis.   Plasmids  
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Use information in nucleoid or plasmid to synthesize proteins. 2 locations free floating in the cytoplasm and associated in the plasma membrane.   Ribosomes  
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Aid the creation of proteins of fully functional proteins. Help fold or add necessary side groups to newly synthesized proteins.   Molecular Chaperons  
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Highly compacted aggregates diverse materials such as proteins glycogen, iron, phosphorus. Funtio is storage.   Granules or Inclusion Bodies  
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General differences from Eukaryotes and Prokaryotes   size, true nucleus, membrane buond organelles, complexity  
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composed of polysaccharides such as chitin, cellulose, glucan   Fungal Wall  
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Primarily composed of polyssaccharides such as cellulose, pectin   Algal Wall  
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usually lack a true exterior cell wall but may have a pellicle= composed of overlapping protein strips just inside the plasma membrane   Protozoan Wall  
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Types of Bulk transport   Exocytosis and Endocytosis  
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Bulk movement out of the cell   Exocytosis  
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Bulk movement into the cell. Solid materials=Phagocytosis, and liquids=pinocytosis   Endocytosis  
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Double-membrane, contains DNA of the cell usually linear molecules of DNA at least 3x the amount in prokaryotic cells   Nucleus  
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Use information from DNA to synthesize proteins, free floating in the cytoplasm used to create proteins inside the cell   Ribosome  
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an irregular network of membrane bound tubules and flattened sacs, used in packaging and distribution.   Endoplasmic Reticulum  
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Has ribosomes associated which packages proteins into membrane bound vesicles for distribution in the cell   Rough E.R.  
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Contains enzymes involved in the creation and degradation of lipids. Packages lipids in membrane bound vesicle for distribution in the cell   Smooth E.R.  
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Composed of flattened sacs called cisterne, receives vesicles from the E.R. modifies the contents into membrane bound vesicles for distribution   Golgi Apparatus  
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Membrane bound vesicles that may contain proteins, carbs, iron, water with dissolved salts. Function is storage.   Vacuole  
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Ways to create Vacuoles   Protein synthesis, and Endocytosis  
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Membrane bound vesicle contains digestive enzymes.   Lysosome  
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"Power house of the cell", generation of ATP through cellular respiration   Mitochondria  
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Use energy from light to create ATP through photosynthesis.   Chloroplast  
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Fiber Types   Microfilaments, Intermidiate filaments, and Microtubules  
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Smallest fibers composed of granular protein subunits called Actin. Function maintain cell shape and create motility by pseudopodia   Microfilaments  
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Composed of fibrous twisted proteins (e.g. keratin) rope-like   Intermidiate Filaments  
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Composed of globular protein subunits called tubulin which create hollow tubelike structure, intracellular movement of oraganelles "tracks", attachment site for DNA during cell division/replication, main structural component in cilia and flagella   Microtubules  
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Function of motility. Hairlike extesions from cell surface both of these contain microtubules. Movement by undulation   Cilia and Flagella  
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Shorter than Flagella, but very numerous usually all over cell surface. (20nm)   Cilia  
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Longer than cilia, but less numerous usually one to a few at one end of the cell. (100-200nm)   Flagella  
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An increase in cell components leads to and increase in cell size which leads to increase in cell number.   Growth  
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Microbial Growth in Eukaryotes   Mitosis  
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Microbial Groth in Prokaryotes   Binary Fission  
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Steps in Binary Fission   1)Cell creates new cell structures.2)Copies DNA which moves to opposite ends of cell.3)Cell creates new plaska membrane & creates 2 daughter cells.4)Daughter cells may separate from one another or remain attached  
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Time required to complete one round of Binary Fission   Generation time (Doubling Time)  
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Batch Culture Pattern   1)Lag phase.2)Exponential Phase.3)Sationary Phase.4)Death Phase  
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First portion of the growth curve.Always has this phase, Cells adjust to environment, cells build new cell components,cells are reparing damage caused during cell transfer   Lag Phase  
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Period of growth with maximum rate of cell number. Cells are most uniform during this phase, best use for experiments/study   Exponential Phase  
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Period of growth with no significant change, nutrients become limited, space becomes limited, waste begins to accumulate.Endospores would be created during this phase. #of cell deaths is = to cell division.   Stationary Phase  
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Final portion of the growth phase, complete lack/depletion of nutrients, waste products become toxic to cells   Death Phase  
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