Help
Options
Didn't know it?
click below
click below
Knew it?
click below
click below
Don't Know
Remaining cards (0)
Know
retry
shuffle
restart
0:00
Embed Code - If you would like this activity on your web page, copy the script below and paste it into your web page.
Normal Size Small Size show me how
Normal Size Small Size show me how
BIO 204 Microbiology
| Question | Answer |
|---|---|
| specialized Disciplines in Microbiology | geomicrobiologists, marine microbiologists, Medical microbiologists, nurse epidemologists, astrobiologists |
| genetic engineering | manipulating the genes of organisms to make new products |
| bioremediation | using living organisms to remedy sn environmental problem |
| biotechnology | production of foods, drugs, and vaccines using living organisms |
| antonie van leeuwenhoek | first to observe living microbes |
| Discovery of spores and sterilization | john tyndall and ferdinad Cohn |
| Discovery of Aseptic techniques | human body is source of infection. -Dr.oliver Wendall -dr.Ignaz semmelweis -joseph lister |
| traditional whittaker system of classification | 5 kingdoms 2 cell types |
| woese-fox system | propose three distinct cell lines -domain bacteria -domain archaea -domain eukarya |
| The six I's | incubation, inoculation, isolation, inspection, information gathering, and identification |
| Specimen collection | sample object of interest anything(body fluids, foods, water, soil, plants, and animals etc.) |
| Inoculation | introduction of a sample into a container of media to produce a culture of observable growth |
| Isolation | -seperating one species from another |
| incubation | under conditions that allow growth |
| Inspection | If a single species is growing in the container, you have a pure culture but if there are multiple species than you have a mixed culture |
| Ways to Identify a Microbe: | -Cell and colony morphology or staining characteristics -DNA sequence -Biochemical tests to determine an organism’s chemical and metabolic characteristics -Immunological tests |
| Chemical composition | synthetic and complex |
| functional type | general purpose, enriched, selctive, differential, anarobic, transport, assay enumeration |
| Physical States of Media | Liquid – broth; does not solidify Semisolid – contains solidifying agent Solid – firm surface for colony formation -Contains solidifying agent |
| Transmission electron microscopes | transmit electrons through the specimen. Darker areas represent thicker, denser parts and lighter areas indicate more transparent, less dense parts. |
| Scanning electron microscopes | provide detailed three-dimensional view. SEM bombards surface of a whole, metal-coated specimen with electrons while scanning back and forth over it. |
| Differential stains | use a primary stain and a counterstain to distinguish cell types or parts (examples: Gram stain, acid-fast stain, and endospore stain) |
| Simple stains | one dye is used; reveals shape, size, and arrangement |
| Acidic dyes | anionic, negatively charged chromophore |
| Nutrient broth | liquid medium containing beef extract and peptone |
| Synthetic | – contains pure organic and inorganic compounds in an exact chemical formula |
| Enriched media | – contains complex organic |
| Complex or nonsynthetic | – contains at least one ingredient that is not chemically definable |
| General purpose media – | grows a broad range of microbes, usually nonsynthetic |
| Selective media | contains one or more agents that inhibit growth of some microbes and encourage growth of the desired microbes |
| Differential media | allows growth of several types of microbes and displays vixible differences among those microbes |
| reducing medium | contains a substance that absorbs oxygen or slows penetration of oxygen into the medium |
| Characteristics of Cells and Life | Basic shape – spherical, cubical, cylindrical Internal content – cytoplasm, surrounded by a membrane DNA chromosome(s), ribosomes, metabolic capabilities |
| Reproduction and heredity | genome composed of DNA packed in chromosomes; produce offspring sexually or asexually |
| Glycocalyx | surface coating |
| Flagella | Filament – long, thin, helical structure composed of protein flagellin Hook – curved sheath Basal body – stack of rings firmly anchored in cell wall |
| Monotrichous | single flagellum at one end |
| Lophotrichous | small bunches emerging from the same site |
| Amphitrichous | flagella at both ends of cell |
| Peritrichous | flagella dispersed over surface of cell |
| Fimbriae | Fine, proteinaceous, hairlike bristles emerging from the cell surface |
Created by:
1185169440
Popular Science sets