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Biochemistry 2 E2
Biochemistry 2 Palmer Exam 2
Question | Answer |
---|---|
what are the enzymes of fatty acid synthesis | 1)acetyl CoA carboxylase 2)fatty acid synthase 3)citrate lyase |
what are the stimulants of fatty acid synthesis | 1)high ATP 2)NADPH, NADH, FADH2 3)citrate 4)insulin |
what inhibits fatty acid synthesis | 1)AMP, ADP(low energy charge) 2)NADP, NAD, FAD 3)palmitoyl CoA 4)catecholamines, glucagon, ACTH, cortisol 5)malonyl CoA |
what is the enzyme for glycogen synthesis | glycogen synthase |
what stimulates glycogen synthesis | 1)glucose 6 phosphate 2)ATP(high energy charge) 3)insulin |
what are the enzymes for gluconeogenisis | 1)pyruvate carboxylase 2)glucose 6 phosphate 3)fructose 1,6 bisphosphatase 4)PEP carboxykinase |
what stimulates gluconeogenisis | 1)low blood glucose 2)glucagon 3)ATP(high energy) 4)pyruvate, lactate, alanine, OAA |
what inhibits gluconeogenisis | 1)high blood glucose 2)insulin 3)AMP, ADP (low energy charge) 4)fructose 2,6 bisphosphate |
what is the function of GLUT 2 | glucose, galactose & fructose -out of the intestine into blood -into the liver -insulin dependent |
what is the function of GLUT 4 | glucose into muscle and adipose tissue -stimulated by insulin -stimulated by low energy charge -facilitated diffusion |
what are 4 main things to know about glucokinase | -high Km -not inhibited by G6P -stimulated by insulin -in liver |
what is the sequence for glucokinase | 1)eat carbs 2)elevated blood glucose 3)elevated insulin 4)fat and muscle glucose transport 5)muscle full of glycogen so glycogen goes to liver 6)glucokinase 7)glycolysis runs 8)energy/raw material 9)fat building |
what are 3 main things to know about hexokinase | -low Km -inhibited by G6P -in muscle |
where is fatty acid synthesis | cytosol |
what 2 molecules are needed to start fatty acid synthesis | 1)acetyl CoA 2)malonyl CoA |
fatty acid synthase CRDR | Condenses (adds 2 carbons) Reduces (with NADPH) Dehydrates Reduces (with NADPH) |
enzymes essential for fructose metabolism | -fructokinase -triokinase |
fructose skips what | regulation of PFK |
conditions that stimulate fatty acid synthesis | 1)high energy 2)lots of acetyl CoA, NADH, FADH2, Citrate, sugar 3)insulin |
what are the key enzymes of fatty acid synthesis | -citrate lyase -acetyl CoA carboxylase -fatty acid synthase (CRDR) |
H ! R-C-COOH ----> ! NH3 (amino acid) | R-C-COOH !! O (keto acid) |
what ends in -Cholate | bile salt, steroid hormone |
HMG CoA reductase is | irreversible |
where are bile salts made | in the liver |
HMG CoA ------> mevalonate what enzyme | HMG-CoA reductase |
storage form of glucose has what kind of bonds | alpha 1,4 or alpha 1,6 |
what are 2 types of starch | -amylose, alpha 1,4 -amylopectin, alpha 1,4 & 1,6 |
where would you store glycogen | cytosol |
where is glucose 6 phosphate | only in the liver |
glucose 6 phosphate is converted to glucose 1 phosphate by what enzyme | phosphoglucomutase |
what enzyme is used for UTP+glucose 1 phosphate ----> UDP-glucose | UDP-glucose pyrophorylase |
what enzyme is used to add glucose to glycogenin | glycogen initiator synthase |
what enzyme is used to add more glucose to the chain of glycogenin | glycogen synthase |
when glucose 1 phosphate is converted to glucose 6 phosphate what enzyme is used | phosphoglucomutase |
what breaks down glycogen | glycogen phosphorylase |
what 2 enzymes are required for cutting down branches of glycogen | -glucosyl(4:4) transferase -amylo-alpha-(1,6)-glucosidase |
what regulates the pathway from glycogen to glucose 1 phosphate | 1)low ATP 2)lots of ADP/AMP 3)low glucose 4)glucagon 5)muscle contraction(calcium) |
what regulates the pathway from glucose 1 phosphate to glycogen | 1)lots of ATP 2)lots of glucose 3)insulin 4)low glycogen levels |
what is the most potent stimulator of glycogen synthase | glycogen levels in muscle |
what are the enzymes used in glycogen synthesis | 1)hexokinase/glucokinase 2)phosphoglucomutase 3)UDP glucose pyrophorylase 4)pyrophosphotase 5)glycogen initiator synthase 6)glycogen synthase 7)glucosyl 4:6 transferase |
what enzymes are used for glycogen breakdown | 1)glycogen phosphorylase 2)phosphoglucomutase 3)glucosyl (4:4) transferase 4)amylo-alpha-(1,6)-glucosidase |
glycogen phosphorylase(liver) during exercise is running faster why? | -muscle contraction uses ATP -muscle low energy charge -muscle stimulates PC, glycolysis, kreb's, electron transport chain -glycogen breakdown -muscle consumes glucose from blood -blood glucose is low -secretes glucagon |
liver energy charge increases during exercise | glycogen synthase=inhibit glycogen phosphorylase=stimulate gluconeogenesis=stimulate glycolysis=inhibit |
what happens when you eat sugar and you are resting | -increase blood glucose -increase insulin -increase [glucose] in cells -stimulate glycolysis -stimulate glycogen synthesis -stimulate fat synthesis |
what is gluconeogenesis | synthesis of glucose from non-carbohydrate precursors |
what are the main entry points into gluconeogenesis | -pyruvate -oxaloacetate -DHAP |
what are the main non-carbohydrate precursors for gluconeogenesis | -lactic acid -amino acid -glycerol |
where is gluconeogenesis taking place | liver 90% and kidney 10% |
what enzymes are responsible for the irreversible reactions of glycolysis | 1)hexokinase 2)phosphofructokinase 3)pyruvate kinase |
WHERE IS PYRUVATE CARBOXYLASE | mitochondrial matrix |
what are the enzymes for glycolysis | -glucokinase -phosphofructokinase -pyruvate kinase |
what are the enzymes for gluconeogenesis | -glucose 6 phosphate -fructose 1,6-bisphosphate -pyruvate carboxylase, PEP carboxykinase |
liver takes up lactic acid and creates | glucose |
what is the primary source of glucose from gluconeogenesis at rest | alpha ketoacids |
what is the corresponding alpha ketoacid with alanine | pyruvate |
what is the corresponding amino acid with pyruvate | alanine |
what are the 3 common ketones | -acetoacetate -beta hydroxybutyrate -acetone |
which would inhibit ketone body formation | -glucose -carbs |
what is the cheerleader for glycolysis, and what does it do | fructose 2,6-bisphosphate, activates PFK in the liver |
what is the exercise sequence for gluconeogenesis | 1)low energy charge 2)+ glycogen breakdown,glycolysis,kreb's, glucose entry in the muscle 3)lower blood glucose 4)secrete glucagon 5)+glycogen breakdown in liver 6)-glycogen synthesis in liver 7)slows glycolysis in liver 8)+ gluconeogenesis in live |
what is the cori cycle | muscle-lactate-GNG in liver-glucose |
what is the glucose alanine cycle | muscle-alanine-GNG in liver-glucose |
what are the steps for the cori cycle | 1)exercis lowers blood glucose 2)glucagon secreted 3)muscle releases lactate 4)lactate goes to liver becomes glucose 5)GNG + by glucagon 6)lactate turned into pyruvate 7)pyruvate turned into glucose 8)glucose goes into blood 9)muscle gets glucose |
what are the steps for the glucose alanine cycle | 1)during exercise 2)glycolysis runs fast 3)AA enters krebs 4)pyruvate from glycolysis takes amino group 5)pyruvate + NH3-->alanine 6)alanine goes to liver,pyruvate turns to glucose, NH3 turns to urea 7)alanine-->pyruvate + NH3 |
where is pyruvate carboxylase | mitochondrial matrix |
what is NOT gluconeogenic | fatty acid |
what is stolen from the krebs cycle to make glucose during ketosis | oxaloacetate |
what is the name of the protein that glycogen attaches to | glycogenin |
low blood sugar, what is stimulated | pyruvate carboxylase & glycogen phosphorylase |