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Streptococcus spp

SCC microbiology

QuestionAnswer
What hemolytic pattern is demonstrated by the Enterococci spp. on a BAP plate? Gamma hemolysis: no hemolysis or discoloration on BAP
What is the clinical significance of the Streptococcus viridens organisms? Commonly associated with being a blood culture contaminant
After streaking a BAP for isolation, why is it good technique to "stab" it 2-3 times in the first quadrant? To enhance hemolytic pattern being demonstrated by Streptococcus spp. on BAP.
What hemolytic pattern is demonstrated by Strep. pneumoniae on a BAP plate? Alpha hemolysis: partical or incomplete hemolysis
Are the Enterococci spp. considered normal flora? If so, where can these organisms be found on the human body? Yes. normal intestinal flora
What is the gram stain reaction and morphology of the Streptococcus spp? gram positive cocci
Describe what a beta hemolytic pattern looks like on a BAP plate. Complete hemolysis which means there is complete lysis of the RBCs in the BAP.
What Lancefield antigen grouping does Streptococcus pneumoniae possess? Strep. pneumoniae has no Lancefield antigen grouping
Describe what an alpha hemolytic pattern looks like on a BAP plate. Partial or incomplete hemolysis meaning a greenish discoloration on the BAP.
Which group of Streptococci/Enterococci spp. are capable of growing well in increased concentrations of bile salts and NaCl, and are also becoming a major problem in the hospital environment? Group D Enterococci spp.
How are Streptococcus organisms grouped using the Lancefield antigens? Grouped by having the same Lancefield antigens located on their surface.
Grouped by having the same Lancefield antigens located on their surface. Capsule formation
What is the clinical significance of Streptococcus agalactiae? pneumonia, sepsis, meningitis in infants, puerperal fever (childbirth fever)
Beta hemolysis on a BAP plate is caused by two substances that cause the complete lysis of RBCs. These substances are known as "hemolysins". Name the two hemolysins. Streptolysin S and Streptolysin O
What is the purpose of knowing about the Lancefield antigens? Used as a technique to identify Streptococcus spp.
Which of the following organisms are commonly associated with community-acquired pneumonia, and exhibit an alpha hemolytic pattern on a BAP plate? Streptococcus pyogenes, Streptococcus agalactiae Streptococcus pneumoniae, Enterococci spp. Which of the following organisms are commonly associated with community-acquired pneumonia, and exhibit an alpha hemolytic pattern on a BAP plate? Streptococcus pyogenes, Streptococcus agalactiae Streptococcus pneumoniae, Enterococci spp.
What hemolytic pattern is demonstrated by the Other Group D Enterococci spp on a BAP plate? Either alpha or gamma hemolysis
What is the clinical significance of Streptococcus pneumoniae? Association: pneumonia, sinusitis, mastoiditis, meningitis, and endocarditis
Where are the Lancefield antigens located on an organism? Located on surface of organism.
which stret spp. shows following characteristics: gpc implicated in pneumonia of newborns, puerperal fever in post-partum women, beta-hemolytic on BAP plate, CAMP test (+)? S.pyogenes, S.agalactiae,S.pneumonia, or Enterococci spp. Streptococcus agalactiae
Which of the common plated media is used to determine the hemolytic patterns of the Streptococus spp.? BAP plate ONLY!
What is the clinical significance of the Enterococci spp.? What is the clinical significance of the Enterococci spp.?
What type of designation is used to group the Lancefield antigens? Alphabet: Group A, Group B, Group C, etc.
What hemolytic pattern is demonstrated by Streptococcus agalactiae on a BAP plate? Beta hemolysis: complete hemolysis
After determining you are dealing with a Streptococcus spp. What is the next logical step in determining the proper identification of the organism? Look at the hemolytic pattern demonstrated on a BAP plate.
Are Streptococcus spp. considered normal flora? If so, where can they be found on the human body? Yes. Found in respiratory, genital, and intestinal tracts.
Are the Other Group D Enterococci spp. considered normal flora? If so, where can these organisms be found on the human body? Are the Other Group D Enterococci spp. considered normal flora? If so, where can these organisms be found on the human body?
Compromised hosts are: Individuals who are more susceptible to opportunistic infections.
What Lancefield antigen grp Strept spp. is responsible for the infection, Necrotizing fasciitis (infects muscle/fat) high death rate quick diagnosis, wound bebrided w/ antibiotics necessary Group A Streptococcus spp.
What is the clinical significance of Streptococcus pyogenes? pharyngitis, rheumatic fever, glomerulonephritis, TSS, necrotizing fasciitis
What Lancefield antigen grouping does Streptococcus pyogenes possess? Group A Lancefield antigen grouping
Describe what a gamma hemolytic pattern looks like on a BAP plate. No hemolysis or discoloration seen on BAP plate.
This organism is the most virulent species in the genus Streptococcus and is responsible for suppurative sequelae such as rheumatic fever and glomerulonephritis. Streptococcus pyogenes
What is the catalase reaction of the Streptococcus spp.? Catalase negative
What hemolytic pattern is demonstrated by Streptococcus pyogenes on a BAP plate? Beta hemolysis: complete hemolysis on BAP plate
What virulence factor do some Streptococcus agalactiae possess that protects them from phagocytosis? Capsule formation
What Lancefield antigen grouping does Streptococcus agalactiae possess? Group B Lancefield antigen grouping
Is Streptococcus pneumoniae considered normal flora? If so, where can it be found on the human body? Yes. Normally found in respiratory tract of humans.
What are the incubation requirements for Streptococcus spp.? Incubation: 37C under increased CO2 concentration
What is the clinical significance of the Other Group D Enterococci spp.? bacteremia, sepsis in newborns, endocarditis and meningitis
Name the three types of hemolytic patterns demonstrated by the Streptococcus spp. Beta, Alpha, and Gamma (BAG-->to remember the order)
What Lancefield antigen grouping do the Enterococci possess? What Lancefield antigen grouping do the Enterococci possess?
What type of environmental conditions are the Group D Enterococci spp. able to survive? Survive in extreme environmental conditions like high % of bile salts & NaCl.
What virulence factor do some Streptococcus pyogenes possess that protects them from phagocytosis? Capsule formation
Created by: Nizhoni T Walter Nizhoni T Walter on 2011-09-25



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