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Worsham Micro Q1
Question | Answer |
---|---|
Microbiology | the study of microorganisms and their effects on other organisms |
eukaryotic | have a true nucleus and membranebound organelles |
prokaryotic | do not have a nucleus or membranebound organelles |
taxonomical hierarchy | Domain, Kingdom, Division/Phylum, Class, Order, Family, Genus, Species |
3 primary Domains | Bacteria, Archaea and Eukarya |
5 Kingdoms | Prokaryote or Monera, Protista, Fungi, Plant and Animal |
Binomial nomenclature | formal system of naming species |
Scientific study of bacteria | bacteriology |
Mycology | scientific study of fungal organisms. |
Rickettsiology | scientific study of rickettsia |
immunology | study of all aspects of the immune system in all organisms |
simple, single-celled organisms that are prokaryotic, a member of the Monera Kingdom, and are generally 1 of 3 shapes | basic characteristics of bacterial cells |
what type of bacteria has no cell wall, is the smallest free-living organism and is intermediate in size between most bacterial cells and viruses | Mycoplasma bacteria |
what type of bacteria is generally involve an insect in disease transmssion to a human host, are obligate intracellular parasites, and cause diseases known as typhus fevers | Richettsia bacteria |
what type if bacteria is an obligate intercellular parasite and has a unique life cycle | Chlamydia bacteria |
what are the characteristics of Protozoa | Eukaryotes (Domain), most are unicellular, the first formed animals(simpleast of animals), and Protista Kingdom |
what are the characteristics of fungal organisms | Eukaryotes (Domain), Fungi Kingdom, examples are mold and yeast |
what are the characteristics of a virus | submicroscopic, acellular, contains a nucleic acid core surrounded by a capsid containing either DNA or RNA |
what does submicroscopic mean | must be seen with an electron microscope |
capsid | protein coat |
obligate intracellular parasites | can only reproduce within a host cell and cannot survive outside the host cell |
CJD | a degenerative nuerological disease that is always fatal |
CJD and mad cow disease are examples of | prions |
characteristics of prions | abnormal infectious protein, very resistant to vacines, typical starilizing is not saficant, and causes diseases known as transmissible spongilform encephalopathies (TSE's) where the brain takes on a sponge-like appearance |
what is the purpose of Gram Stain and Acid-fast stain | to identify types of bacteria |
Gram-negative bacteria means | they tend to be more resistant to penicillin |
what genus is Acid-fast used to id | Mycobacterium |
Leparacey and tuberculosis is identified by | Acid-fast stain |
morphology | the study of shape and form without regard to function |
bacteria is measured in | micrometers |
3 primary shapes of bacteria | sphereical, rod-shaped & spiral |
cocci (coccus) are | sphereical or berry shaped |
Staphylococcus aureus (skin abscess) and Streptococcus pyogenes (strep throat) are what shape | cocci or sphereical |
bacillus (bacilli) are | rod-shaped or colum like |
Bacillus anthracis (anthrax), Salmonella typhi (typhoid fever), Shigella olysenteriae (shigellosis) and Clostridium tetani (tetanus are what shape | bacillus or rod-shaped |
vibrio are | comma-shaped |
spirochetes are | helical (flexible when in motion) |
spirillum are | helical (rigid when in motion) |
monomorphic | does not change shape |
pleomorphic | does change shape |
Most bacteria are monomorphic, T/F | True |
diplococci | cocci that remain in pairs |
streptococci | cocci that remain attached in a chainlike pattern after dividing |
tetrads | cocci that divides in 2 planes & remain in groups of 4 |
sarcinae | cocci that divides in 3 planes and remain attached in cube-like groups of 8 |
staphylococci | cocci that divides in multiple planes and form grapelike clusters |
diplobacilli | bacilli that divides across the short axis and remain in pairs |
strptobacilli | bacilli that divides across their short axis and remains in chains |
coccobacilli | bacilli are oval and look so much like cocci they are called coccobacilli |
capsule | closely attached and organized outer layer of the cell wall |
slime layer | loosely attached and unorganized outer layer of the cell wall |
glycocalyx | a sticky, gelatinous coating secreted that surround the cell wall |
how does the capsule or slime layer enhance virulence | resisting phagocytosis |
this type of bacteria has a capsule | Streptococcus pneumoniae |
flagella | long, whiplike, filament-containing appendages that allow bacteria to move (motility) |
monotrichous | single polar flagellum |
amphitrichous | flagellum or flegella at both ends of a bacteria |
lophotrichous | tufts or bunches of flagella at one end of the bacteria |
peritrichous | flagella covering the entire surface |
taxis is | movement toward or away from a stimulus |
phototaxis | bacterial stimulus is light |
chemotaxes | bacterial stimuli includes chemicals |
axial filaments or endoflagella | unique type of flagella produced by spirochetes |
an example of a bacteria that produces axial filaments is | Treponema pallidum (syphilis) |
pili (fimbriae) | short hairlike or bristle-like appendages that allow bacterica to attach to surfaces |
an example of bacteria that produces pili is | Neisseria gonorrhoeae (gonorrhea) |
What are the main functions of a bacterial cell wall | prevents rupturing when the water pressure inside is greater then outside and helps maintain the shape of the bacterium |
peptidoglycan | primary macromolecular network of a bacterial cell wall |
A Gram-Pos cell wall has many layers or few layers of peptidoglycan | many |
teichoic acids are found in Gram-Pos or Gram-Neg | Gram-Pos |
A Gram-Neg cell wall has many layers or few layers of peptidoglycan | few |
Gram-Neg cell wall consists of | lipopolysaccharides, lipoproteins, and phospholipids |
What is the purpose of lipopolysaccharides, lipoproteins and phosphlipids in what type of cell | helps evade phagocytosis in Gram-Neg |
Which is more susceptible to mechanical breakage, Gram-Pos or Gram-Neg | Gram-Neg |
What is the lipid portion of lipoplysaccharides called and referred to as | Lipid A and endotoxin |
When is endotoxin released | when there is bacterial lysis |
What are the 2 primary types of toxins | endotoxin and exotoxin |
what is an example of an atypical bacterial cell | Mycoplasma |
what is selective toxicity | when the chemical difference in the bacterial cell wall from the host cells so that antibiotics will target the bacteria and not the host's cells |
What is the digestive enzyme that can damage the bacterial cell wall that is present in saliva and mucus | lysozyme |
Which cell wall is almost completely destroyed by lysozyme | Gram-Pos |
What is a wall-less cell is called | portoplast |
When lysozyme is present a Gram-Neg the cell wall is | not completely destroyed |
spheroplast is | what remains of a Gram-Neg cell and its wall after lysozyme |
where is the plasma membrane located | inside the cell wall, enclosing the cytoplasm of the cell |
what is the most important function of the plasma membrane | determines what substances exit and enter the cell |
What are the types of movement of materials across membranes | passive and active |
when substances move with the concentration gradient, is that passive or active | passive |
how much ATP is expended in passive transport | none |
how do substances move against the concentration gradient | active transport and use of ATP |
what is simple diffusion | the net movement of molecules or ions from an area of high concentration to an area of low concentration through passive transport |
what is facilitated diffusion | substances are transported through the plasma membrane by using a carrier protein from high concentration to low concentration |
carrier proteins are also known as | transporter or protease |
what is osmosis | the simple diffusion of water |
what is a solvent | the substance the solute is being desolved in |
what is a solute | the substance being desolved |
what is a solution | the mixture of the solvent and solute |
what are the 3 types of osmotic solutions a bacterial cell may be subjected to | isotonic, hypotonic or hypertonic |
isotonic, hypotonic and hypertonic are describing the | solute concentration outside the cell |
when the solute concentration outside the cell is equal to the solute concentration inside the cell the cell is in what type of solution | isotonic or in equilibrium |
what is a hypotonic solution | when the solute concentration outside the cell is lower than the solute concentration inside the cell |
when a cell is in a hypotonic solution it may burst or collapse | expand and burst |
plasmoptysis or osmotic lysis is | when a cell expands and bursts |
hypo means | less or under |
hyer means | above or more |
what is a hypertonic solution | when the solute concentratin outside the cell is high then the solute concentratin inside |
when a cell is in a hypertonic solution it may burst or colapse | shrink and collapse |
when a cell undergoes plasmolysis it | shrinks and collapses |
in active transport is the the substance being transported chemically changed? | no |
what type of cells use group translocation and what is different about it | prokaryotic cells and the substance is chemically altered |
in a prokaryotic cell where is the cytoplasm | inside the plasma membrane where all major structures are located |
what are inclusions or inclusion bodies | temporary storage of ATP and other nutriants, controls meterial being released throughout the cell |
what are 3 other names for nuclear area | nuclear body, nuclear region, nucleoid |
what is the function of ribosomes | protein synthesis |
is the creation of an endospore a reproductive or a defense mechanism | defense or protective |
what type of cells create endospores, Gram-Pos or Gram-Neg | Gram-Pos |
What shape are the bacteria that create endospores | rod-shaped |
what is the process of spore formation called | sporulation or sporogenisis |
non-spor state is called | vegetative state |
germination of a spor is | going from spor to vegetative |
what are the important characteristics of the bacterial cell wall | prevents rupture, helps maintian shape & integrity, point of anchorage for flagella, produces peptidoglycan |
what type of cell has many layers of peptidoglycon superior to plasma membrane and contains teichoic acids | Gram-pos |
what type of cell only has 1 or few layers of peptodoglycan & an outer membrane, consists of lipopolysaccharides (LPS), lipoproteins & phosholipids and products 2 types of toxins | Gram-neg |
which is more susceptible to mechanical breakage, Gram-pos or neg | Gram-neg |
which is better at evading phagocytosis | Gram-neg |
Lipid A is referred to as | an endotoxin |
endotoxins are not released unless | there is bacterial lysis |
what are the 2 types of toxins | endotoxins and exotoxin |
endotoxins are apart of | the Gram-neg cell wall |
exotoxins are secreated | to the outside of the cell |
protoplast | wall-less cell when the cell wall is destroyed by lysozyme in Gram-pos cells |
spheroplast | when the cell wall is not completely destroyed by lysozyme in Gram-neg cell |
where is the plasma membrane of a bacteria cell located | internal to the cell wall and enclosing the cytoplasm of the cell |
what is the primary function of the plasma membrane of the bacteria cell | determines what substances exit and enter the cell |
what are 2 secondary functions of the plasms membrane of the bacteria cell | enzymatic breakdown of nutrients and enzymatic production of ATP |
passive transport | allows substances to cross the membrane from an area of high concentration to an area of low concentration with the concentration gradient, no energy is used |
active transport | movement of substances in and out of the cell against the concentration gradient, using energy |
simple diffusion | net movement of molecules or ions from an area of high concentration to low concentration |
facilitated diffusion | movement of larger molecules from higher to lower areas of concentration by using a carrier protein (protease) |
osmosis | simple diffusion of water from an area of high concentration to low concentration |
what effect would an isotonic solution have on the bacterial cell wall | none, it would be in equilibrium |
what effect would a hypertonic solution have on the bacterial cell wall | the cell would shrink, collapse or undergo plasmolysis. concentration outside is higher then inside |
what effect would a hypotonic solution have on the bacterial cell wall | expand & burst; plasmoptysis; osmotic lysis. concentration outside is lower then inside |
what type of cell uses group transport | prokaryotic cells |
what type of transport chemically changes during the transport process | group transport |
what types of transport use ATP | group and active |
what type of transport moves molecules against the concentration graident | active transport |
where is the cytoplasm in a prokaryotic cell | inside the cell wall & plasma membrane |
where is teh cytoplasm in a eukaryotic cell | inside the cell wall & plasma membrane but outside the nueclus |
where is the cell's genetic info located | vuclear body (nuclear area, nuclear region, uncleoid) |
what structure is responsible for protein synthesis | ribosomes |
function of the inclusion bodies | temporary storage of nutrients |
what shape are endospores | Rod-shaped |
What genera form endospores | Clostridium and Bacillus |
what would make a bacterial cell form an endospore | the environment for the bacterial cell have changes so drasticly, the cell cannot survive |
what is the non-spore state of an endospore forming cell | vegetative |
when returning to vegetative state | germination |
is an endospore is created is it for reproduction or protection | protection |
psychrophile | bacteria that prefer cold, thriving at temps between 0-25 degrees C |
mesophile | bacteria that prefer moderate temps and develop best at temps between 25-40 degrees C |
thermophile | bacteria that thrive best at high temps between 40-70 degrees C |
temp below which bacterial growth will not take place | minimum growth temperature |
temp above which bacterial growth will not take place | maximum growth temperature |
temp at which organisms grow best | optimum growth temperature |
what is the optimum pH for bacterial growth | near neutral, range from 6-8 |
there is no net flow of water in or out of the cell when | osmotic pressure is high |
what are major nutrient requirements for bacteria | water, carbon, nitrogen, sulfur, phosphorus, iron, copper, molybdenum and zinc |
why is water important to bacterial growth | all require moisture but not all need the same amount |
why is carbon important to bacterial growth | structural backbone and one of the most important requirements for growth |
why are N,S,P,Fe, Cu, Zn & molybdenum important for bacterial growth | needed by the cell for protein synthesis, synthesis of DNA, RNA & ATP |
bacteria that require complex organic food from a carbon source to grow & develop or "other feeder" | heterotroph-most pathogenic bacteria |
self-nourishing bacteria capable of growing in the absence of organic compounds, uses carbon dioxide | autroph-plants |
completely dependant on their living host for nutrients they need to survive | obligate parasite |
survives on dead or decaying organic matter | obligate saprophyte |
facultative parasite | has the ability to adpat and survive on living organic matter but prefers dead or decaying organic matter |
facultative saprophyte | has the ability to adapt and survive on dead or decaying organic matter but perfers living organic matter |
obligate aerobe | microorganisms that can only live in the presence of oxygen, because they need it to metablolize sugars |
obligate anaerobe | microbes that can only survive in an environment devoid of oxygen |
adapted to survival in the presence of oxygen but prefers the live without oxygen | facultative aerobe |
adapted to survival without oxygen but prefers the presence of oxygen | facultative anaerobe |
microaerophile | microorganisms that require little free oxygen (2-10%) |
microorganisms that thrive/require the presence of high concentrations of carbon dioxide, low oxygen | capnophile |
bacterial colony | visible group of bacteria growing on a culture medium |
more then 1 species | mixed colony |
pure colony | only 1 species |
simple transverse division | binary fission |
binary fission | method of asexual reporduction in bacteria in which the parent cell splits into 2 parts (daughter cells) |
time required for a cell to divide (population to double) | generation time |
bacterial growth calculation equation | Bf=Bix2N |
Bf is | final bacterial population after a determinded amount of time |
Bi is | given value (number of bacterial cells) |
N is | number of generations |