Using a 25 gauge needle with a 10ml evacuated tube may cause:
hemolysis
How does the anticoag in the green stopper tube work:
by inhibiting thrombin in the coag cascade
Name 3 anticoags that prevent clotting by binding calcium and the color coded stopper associated with them:
sodium citrate- light blue EDTA- lavendar Sodium citrate-black
What is the purpose of sodium flouride in a gray stopper tube:
Maintains glucose stability for 3 days
Why is EDTA the anticoag of choice for the CBC:
Maintains cellular integrity better that the other anticoag: inhibits platelet clumping- doesn't interfere with routine staining procedures
What color tube that must always be completely filled:
light blue
What is the purpose of tapping an evacuated tube containing dried anticoag before using it:
loosen the powder from the tube for better results
Which of the following tubes will clot first: red, gold, or orange
Orange
Under what circumstances should the amount of anticoag in a light blue stopper tube be decreased:
Collecting on patients with polycythemia or hematocrit reading less than 55%
Why are royal blue stopper tubes used for collecting trace metal analyses:
tubes are chemically cleaned and rubber stoppers are specially formulated to contain the lowest possible metal levels
When are winged infusion sets used in phlebtotomy:
very small veins- children and geriatrics
Syringes are graduated in:
mL and cc (milliliter/cubic centimeter)
When a blood pressure cuff is used as a tourniquet how should the pressure be adjusted:
below the systolic bp but above the diastolic bp
List 2 precautions a phlebotomist would take when collecting a blood specimen from a patient with a latex allergy:
use latex free tourniquet, gloves and bandages
List 2 antiseptics used in venipuncture and a state a situation when each is used:
70% isopropyl alcohol- to prevent contamination by normal skin bacteria during specimen collection iodine/chlorhexidene gluconate- additional sterility for collections such as arterial punctures and blood cultures
Anticoag- sodium heparin, ammonium heparin or lithium heparin Purpose- prevents clotting by inhibiting thrombin Tests- STAT lytes Interference-should not be used for hematology because it interferes with the Wrights stained blood smear
Light green Hemoguard/green & black stopper tubes
Anticoag-lithium heparin and separation gel Purpose-Plasma determinations in chemistry Tests- Potassium Inverted-x8
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