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micro test 3
summary Q
| Question | Answer |
|---|---|
| better growth on SBA than MAC Oxidase +, TSI (K/K) | Suspect a NF |
| found where moisture accumulates, all have pyoverdin | Pseudomonads |
| Cystic fibrosos, Jacuzzi/hot tub syndrome, swimmers ear | P.Growth on SBA, MAC, CHOC(green/yellow colonies), 47 days aeruginosa |
| Virulence factors: attachment, invasion, toxins | P. aeruginosa |
| lab ID: lg colonies, pyocyanin, “grape-like odor”, Oxi +, TSA +(green slant), growth at 42 C, Glucose +, Citrate + | P. aeruginosa |
| needs 2x grug therapy | P. aeruginosa |
| differentiated by gelatin hydrolysis | P. flurescens +, P. putida (nasty odor) = |
| soil denitifier | P. stutzeri |
| used to belong to Pseudomonas | Burkholderia |
| onion bulb rot (plant pathogen), found in IV fluids, detergents, disinfectants | Burkholderia cepacia |
| Growth on SBA, MAC, CHOC(green/yellow colonies), 47 days | Burkholderia cepacia |
| motile+, lysine/ornathine+, glucose/maltose/lactose/mannitol+ | Burkholderia cepacia |
| “glanders”(respiratory disease in animals), NON-MOTILE | B. mallei |
| meliodosis (aggressive granelomatous pulmonary disease) | B. psuedomallei |
| 3rd most NF in nosocomial infections, aerobic, motile by flagella, implants self in medical devices | Stenotrophomonas maltophilia |
| -“ammonia odor” , Oxi/Cat + , Esculin +, Maltose+++++(strong) | Stenotrophomonas maltophilia |
| MAC growth (purple), Oxi=, Cat + , Nonmotile nonmotile | Acinetobacter |
| seen in nosocomial infectons: ICU | Acinetobacter |
| contamination, nonsaccharolytic | A.iwoffii |
| saccharolytic (glucose +) | A. Baumannii |
| live in seawater thus Halophilic except V. chorela | Vibrio |
| Oxi+, MAC growth, comma-shaped/curved rods | Vibrio |
| cause CHOLERA a water borne illness (severe gastroenteritis) found in plankton, fresh, blackish, and salt water | V. cholera |
| cholera toxin/Choleragen; “rice water stool” | V. cholera |
| non hemolytic, VP= : classic V. cholera 01 beta-hemolytic, VP+ : el tor V. cholera 01 yellow on TCBS, sucrose+ | V. cholera |
| growth 1-2% NCl, gastroenteritis from seafood(shell fish/ousters), TCBS green(sucrose=) | V. parahemolyticus |
| wound infections then septicemia from raw shellfish/oysters, lactose+: | V. vulificus |
| Oxi+, glucose+, found in flesh water, warm/cold blooded animal disease, motile by 1 flagella, grow 0-42 C | Aeromonas |
| MAC/SBA(beta) growth, Oxi/Ind+ aerolysin (cytotoxic enterotoxin) | A. hydrophilia |
| found in fresh water, growth SBA/MAC, motile | Plasiomonas |
| cause gastritis from cold blooded animals | P. shigelloides |
| -“darting motility”,curved/S shaped, “seagull shape” most common cause of diarrhea | Capylobacter jejuni |
| microphilic, Cat/Oxi+, NLF, growth 42 C, Hippurate hydrolysis +, Campy-blood agar(sodium bisulfide) | Capylobacter jejuni |
| curved, microaerophilic, high humidity Cat/Oxi/Urease+; EIA, urease test on biopsy, direct stain, stool antigen test | Helibacter pylori |
| ulcers, gastric cancers | Helibacter pylori |
| motile, Oxi + , growth MAC NLF, Nitrite to gas | Alcaligenes faecalis |
| feather edge, “fruity” odor, groth in 6.5% NaCL | Alcaligenes faecalis |
| -motile respiratory pathogens | Bordetella bronchiseptica |
| non-motile respiratory pathogens | Bordetella pertussis/parapertussis |
| pertussin toxin, tracheal cytotoxin, hemoaggutination | Bordetella |
| catarrhal/paradxysmal/convalescent stage | pertussis toxin |
| -temporal vaccine available -DFA/culture from nasal specimen | Bordetella |
| Bordet-Genou/Regan-Lowe | Bordetella |
| milder pertussis, nontoxigenic strain, Cat+Oxi=Urea+(24 hrs) | B. parapertussis |
| Kennel cough in dogs, growth SBA/MAC, Cat/Oxi+Urea+(4 hrs): | B. bronchiseptica |
| Cat/Oxi +, 20-43 C temp. range | L. pneumophilia |
| -Cysteine/iron, BCYE -found in water ex. Lakes/air conditioners, lives/multiply in protozoa/bronchial macrophages | Legionella |
| -get from breathing in: Legionnaires disease(pneumonia)/ Pontiac fever -urine test detects antigen, DFA, serological | Legionella |
| zoonotic disease | Brucella |
| (most virulent) from sheep/goat | B. melitensis |
| Cat/Oxi +, no carb., Brucella agar (5% horse/rabbit serum), “fine grains of sand” on gram stain | Brucella |
| -survives RES system -Brucellosis, Undulant, Mediterranean, Malta fever | Brucella |
| -get from dairy products, contact from animal -serology is the gold standard | Brucella |
| -growth on SBA/CHOC, no MAC -Oxi/Cat/Ind +; Urea =;“sick TSI” | Pasteurella multocida |
| -“musty” “mushroom” odor -get from animal contact esp. cat/dog bite (Cellulitis), respiratory tract/eye infect | Pasteurella |
| -leading cause of endocarditic within genus -Cat/Oxi =, Nitrate +, glucose/lactose fermented | Hemophilus aphrophilus |
| -dental infections; Cat + Oxi = -poor growth on SBA/CHOC, adhere to agar, “pointed star” | Actinobacillus actinomycetemconitans |
| -associated exclusively with endocarditis -rosettes on gram stain -Oxi/Ind + ; cat/Nit = | Cardiobacterium hominis |
| -fist fight/bite “clenched fist wound” -pits agar, “bleach odor” -Oxi/Nit + ; non motile ; non saccharolytic | Eikenella corrodens |
| --plumb rods in chains; Oxi + Cat = ; ferment glucose -flora of pharynx in young children; Remel Rapid NH | Kingella spp. denitrificans/kingae |
| better growth on SBA than MAC Oxidase +, TSI (K/K) | Suspect a NF |
| found where moisture accumulates, all have pyoverdin | Pseudomonads |
| Cystic fibrosos, Jacuzzi/hot tub syndrome, swimmers ear | P.Growth on SBA, MAC, CHOC(green/yellow colonies), 47 days aeruginosa |
| Virulence factors: attachment, invasion, toxins | P. aeruginosa |
| lab ID: lg colonies, pyocyanin, “grape-like odor”, Oxi +, TSA +(green slant), growth at 42 C, Glucose +, Citrate + | P. aeruginosa |
| needs 2x grug therapy | P. aeruginosa |
| differentiated by gelatin hydrolysis | P. flurescens +, P. putida (nasty odor) = |
| soil denitifier | P. stutzeri |
| used to belong to Pseudomonas | Burkholderia |
| onion bulb rot (plant pathogen), found in IV fluids, detergents, disinfectants | Burkholderia cepacia |
| Growth on SBA, MAC, CHOC(green/yellow colonies), 47 days | Burkholderia cepacia |
| motile+, lysine/ornathine+, glucose/maltose/lactose/mannitol+ | Burkholderia cepacia |
| “glanders”(respiratory disease in animals), NON-MOTILE | B. mallei |
| meliodosis (aggressive granelomatous pulmonary disease) | B. psuedomallei |
| 3rd most NF in nosocomial infections, aerobic, motile by flagella, implants self in medical devices | Stenotrophomonas maltophilia |
| -“ammonia odor” , Oxi/Cat + , Esculin +, Maltose+++++(strong) | Stenotrophomonas maltophilia |
| MAC growth (purple), Oxi=, Cat + , Nonmotile nonmotile | Acinetobacter |
| seen in nosocomial infectons: ICU | Acinetobacter |
| contamination, nonsaccharolytic | A.iwoffii |
| saccharolytic (glucose +) | A. Baumannii |
| live in seawater thus Halophilic except V. chorela | Vibrio |
| Oxi+, MAC growth, comma-shaped/curved rods | Vibrio |
| cause CHOLERA a water borne illness (severe gastroenteritis) found in plankton, fresh, blackish, and salt water | V. cholera |
| cholera toxin/Choleragen; “rice water stool” | V. cholera |
| non hemolytic, VP= : classic V. cholera 01 beta-hemolytic, VP+ : el tor V. cholera 01 yellow on TCBS, sucrose+ | V. cholera |
| growth 1-2% NCl, gastroenteritis from seafood(shell fish/ousters), TCBS green(sucrose=) | V. parahemolyticus |
| wound infections then septicemia from raw shellfish/oysters, lactose+: | V. vulificus |
| Oxi+, glucose+, found in flesh water, warm/cold blooded animal disease, motile by 1 flagella, grow 0-42 C | Aeromonas |
| MAC/SBA(beta) growth, Oxi/Ind+ aerolysin (cytotoxic enterotoxin) | A. hydrophilia |
| found in fresh water, growth SBA/MAC, motile | Plasiomonas |
| cause gastritis from cold blooded animals | P. shigelloides |
| -“darting motility”,curved/S shaped, “seagull shape” most common cause of diarrhea | Capylobacter jejuni |
| microphilic, Cat/Oxi+, NLF, growth 42 C, Hippurate hydrolysis +, Campy-blood agar(sodium bisulfide) | Capylobacter jejuni |
| curved, microaerophilic, high humidity Cat/Oxi/Urease+; EIA, urease test on biopsy, direct stain, stool antigen test | Helibacter pylori |
| ulcers, gastric cancers | Helibacter pylori |
| motile, Oxi + , growth MAC NLF, Nitrite to gas | Alcaligenes faecalis |
| feather edge, “fruity” odor, groth in 6.5% NaCL | Alcaligenes faecalis |
| -motile respiratory pathogens | Bordetella bronchiseptica |
| non-motile respiratory pathogens | Bordetella pertussis/parapertussis |
| pertussin toxin, tracheal cytotoxin, hemoaggutination | Bordetella |
| catarrhal/paradxysmal/convalescent stage | pertussis toxin |
| -temporal vaccine available -DFA/culture from nasal specimen | Bordetella |
| Bordet-Genou/Regan-Lowe | Bordetella |
| milder pertussis, nontoxigenic strain, Cat+Oxi=Urea+(24 hrs) | B. parapertussis |
| Kennel cough in dogs, growth SBA/MAC, Cat/Oxi+Urea+(4 hrs): | B. bronchiseptica |
| Cat/Oxi +, 20-43 C temp. range | L. pneumophilia |
| -Cysteine/iron, BCYE -found in water ex. Lakes/air conditioners, lives/multiply in protozoa/bronchial macrophages | Legionella |
| -get from breathing in: Legionnaires disease(pneumonia)/ Pontiac fever -urine test detects antigen, DFA, serological | Legionella |
| zoonotic disease | Brucella |
| (most virulent) from sheep/goat | B. melitensis |
| Cat/Oxi +, no carb., Brucella agar (5% horse/rabbit serum), “fine grains of sand” on gram stain | Brucella |
| -survives RES system -Brucellosis, Undulant, Mediterranean, Malta fever | Brucella |
| -get from dairy products, contact from animal -serology is the gold standard | Brucella |
| -growth on SBA/CHOC, no MAC -Oxi/Cat/Ind +; Urea =;“sick TSI” | Pasteurella multocida |
| -“musty” “mushroom” odor -get from animal contact esp. cat/dog bite (Cellulitis), respiratory tract/eye infect | Pasteurella |
| -leading cause of endocarditic within genus -Cat/Oxi =, Nitrate +, glucose/lactose fermented | Hemophilus aphrophilus |
| -dental infections; Cat + Oxi = -poor growth on SBA/CHOC, adhere to agar, “pointed star” | Actinobacillus actinomycetemconitans |
| -associated exclusively with endocarditis -rosettes on gram stain -Oxi/Ind + ; cat/Nit = | Cardiobacterium hominis |
| -fist fight/bite “clenched fist wound” -pits agar, “bleach odor” -Oxi/Nit + ; non motile ; non saccharolytic | Eikenella corrodens |
| -plumb rods in chains; Oxi + Cat = ; ferment glucose -flora of pharynx in young children; Remel Rapid NH | Kingella spp. denitrificans/kingae |
| -tularemia(get from animal carcasses, biting ticks),use safety BSC III | Francisella tulrensis |
| -BCYE (cystine/iron), CHOC+, slow growth (25 days) -sm green drop like colonies -Cat + , ELISA, PCR | Francisella tulrensis |
| -most normal flora of upper respiratory tract -DO NOT refrigerate,“blood lover”, Oxi/Cat + | Haemophilus |
| -tiny, nonmotile gram neg. coccobacilli - X (hemin), V (NAD): CHOC provides both | Haemophilus |
| -affects children spread by droplets and close contact -needs X & V | H. influenza |
| -virulent factors:polysaccharide capsule, capsule type B , IgA proteases -non-encapsulated strain: adherence, lipoliosaccharide (LOS) | H. influenza |
| satellites around S. aureus on SBA (sm translucent colonies) | H. influenza |
| - “pink eye”(conjunctivitis) -Brazilian purpuric fever -needs X & Y -Remel Rapid NH ID (4 hrs) | H. aegyptius |
| -STD: genital ulcers/chancres/chancroid -X factor only -plate immediately, high humidity, growth 2-14 days, “school of fish”, Cat = , Oxi + | H. ducreyi |
| V only | H. parainfluenzae |
| -X & V -Beta hemolysis in rabbit/horse BA | H. haemolyticus |
| gram neg. diplococcic ”kidney shaped” Oxi/Cat +, CHOC for isolation | Neisseria |
| sexually transmitted | N. gonorrhaeae |
| -males asymptomatic - PID (pelvic inflammatory disease) | N. gonorrhaeae |
| -virulence: pili, IgA protease, capsule -specimen: genital sites, use Dacon/Rayon swab, plate immediately onto CO2 generators & bags | N. gonorrhaeae |
| -MTM (Modified Thayer Martin)-antibiotics inhibits other bacteria -ID: gram neg. diplococcic, CHOC growth, not SBA, Cat/Oxi + | N. gonorrhaeae |
| -affects adolescents(college dorms) -vaccine -community acquired meningitis | N. Meningitidis |
| -strains: A,B,C,Y, W135 -virulence: pili, capsule, endotoxins, IgA protease -specimen: CSF, blood, skin scrapings | N. Meningitidis |
| -ID: growth CHOC/SBA/MTM, Cat/Oxi + CTA (cystine trypticase soy)-red + -BBL crystal NH ID -DNA prove | N. Meningitidis |
| -normal in respiratory tract, pathogenic if found in sterile site | N. lactamica, N. sicca, N. subflava |
| -gram neg. diplococci -growth in SBA, no MAC -normal respiratory flora -Oxi/Cat + -Nitrate reduction + -Asacchrolytic | Moraxella catarrhalis |
| causes abortion/sterility in animals | Campylobacter jejuni |
| causes whooping cough | Bordetella perussis |
Created by:
istophane
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