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micro lab 22B 23A
Transformation of E coli with Plasmid DNA and intro to viruses phage typing
| Question | Answer |
|---|---|
| method of genetic exchange involving the transfer of naked DNA from dead donor cells to living recipient cells | transformation (DNA mediated transformation) |
| ___ is restricted to bacteria that are closely related and does not occur often | transformation of genetic exchange |
| Only cells that are ___ in logarithmic stage of growth undergo transformation | competent |
| Bacteria that are ___ will readily pick up plasmid DNA | competent |
| pUC19, pBR322, pGEM and pGLO carry genes called___ that code for resistance to ampicillin | marker genes |
| ___ allow for the detection of transformed cells on media with ampicillin antibiotic | marker genes |
| the gene providing ampicillin resistance encodes for the enzyme | Beta lactamase |
| __ is an enzyme catalyzing the hydrolysis of beta lactam antibiotics (penicillins and cephalosporins) | Beta lactamase |
| the structural genes of pGLO have a gene encoding for ____ originally produced by jellyfish | green fluorescent protein |
| gells carry pGLO plasmid will produce ___ when on media containing ampicillin and arabinose | green fluorescent protein |
| __ are cells able to take in DNA from the environment from closely related cells | competent cells |
| non cellular, macromolecular comples with a nucleic acid core surrounded by a caspsid | virions |
| infective form of a viruse particle as it exists outside the host cell | virions |
| all viruses are obligate intracellular parasites or __ | hypotrophs |
| __ are ultramicroscopic life forms and infect and reproduce within specific host cells | viruses |
| biruses ata infect bacteria are called | bacteriophages |
| when bacteria and cytolytic bacteriophages are both on agar the destruction shows as windows called | plaques |
| __ means cell lysing | cytolytic |
| bacterial identification method that involves known bacteriophages to identify unknown bacteria | phage typing |
Created by:
JohnPink
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