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Immuno Exam 1 Lab

What kind of tissue is blood? Connective
Blood is composed of? Formed elements (45% RBCs and a buffy coat with WBCs/platelets) and fluid component aka plasma (55%)
Blood formed elements? RBCs and WBCs
Buffy coat Forms between plasma (on top in tube) and RBCs (bottom of tube). Take up “no” percentage due to rounding
Erythrocytes= RBCs
Leukocytes= WBCs
What are the two types of Leukocytes? Granulocytes and Agranulocytes
Granulocytes Have a lot of granules that they use to hold and digest stuff like microbials. **Neutrophils, eosinophils, basophils
Agranulocytes Have few granules. They are multi-lobed. **Lymphocytes, monocytes
Thrombocytes= Platelets
High or low counts in Wright’s Stains are associated with? Infections, allergic reactions, leukemia’s
Order of normal blood cell counts from greatest to least? Leukocytes, Neutrophils, Lymphocytes, monocytes & eosinophils & basophils are in low numbers
WBCs do what if invaders get in body? • If invaders get in the body, then WBCs begin their attack • With the right chemical signals, they will migrate into tissue. Otherwise they migrate around the body
Lymphocytes • Includes both T and B cells- indistinguishable • 8-10 um in diameter • Round nucleus, occupies most of the cell • Few organelles and mostly inactive chromatin • Tell them apart because it is a little bigger then RBCs and mostly made of nucleus
Thrombocytes • 2-3 um in diameter • Look like excess stain • Lifespan= 5-9 days • Function= blood clotting*
Blood smears made 1) 1 small drop on slide 2) Use second slide to smear blood thinly
Wright's Stain Used to differentiate blood cell types; Mix of eosin (red) & methylene blue dyes; performed if overall number of leukocytes is high or low(tells infection); Used to help determine whether it is bacterial infection or allergies, counts the amount of WBCs
Describe RBCs >99% cells in blood;6-8um; biconcave-shape disk; anucleate; lose nucleus; hemoglobin to carry O2 in and CO2 out; lifespan 120 days; 5-5.5 cells per drop
Describe Neutrophils 60-70% of WBCs, recruited by inflame. signals from innate cells; phagocytic; release antimicrobials&tissue remodeling; life 1-2 days; self-destruct as destroying; polymorphonuclear
What proteins do neutrophils release? antimicrobials and tissue remodeling proteins
Neutrophils granules 1) very specific and do not pick up red/blue dye 2) 1/3 called Azurophilic granules (lysosomes), magenta color- name from die
Describe Eosinophils 1-3% circulating leukocytes, phagocytic; defense against multicellular; 12-17um; bilobed nucleus hard to see due to die, many stained red granules, life 8-12days
Describe Basophiles <1%; 8-10um; bilobed nucleus; graules are blue, contain heparin and histamine, produced when injured; part of allergic response
Describe Monocytes 5-10%; circulate in few hours; migrate into tissue and differentiate into phagocytic cells (macrophages and dendritic); 10-30um; tissue specific; large kidney shape; LINK INNATE AND ADAPTIVE
Describe Lymphocytes include both T/B cells; 8-10um; round nucleus; few organelles & inactive chromatin; bigger than RBCs and mostly made of nucleus
Created by: s514149