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Lab Quiz Three

Microbiology Lab

Steps before working with Bacteria? Step 1-5. 1. Hair pulled back before entering Lab. 2. Examine hands and wrist for broken skin and Band-Aid if needed. 3. Put on gloves before touching anything. 4. Disinfect work area before sitting anything on it. 5. Put on Lab Coat.
How to Disinfect Work area? Steps 1-3. 1. Use Baccdown., squirt area that is needed. 2. Use paper towel to spread over entire surface. 3. Allow time to air dry, this would be the time to put on lab coat.
Steps after working with Bacteria? Steps 1-6. 1.Place all biohazard waste in autoclave can. 2.Place all biohazard slides in biohazard box. 3.Remove all items from bench. 4.Disinfect bench with gloves still on. 5.Remove gloves and place them in autoclave can. 6.Wash hands with antimicrobial soap.
What is the most dangerous culture and why? Broth culture, because its easy to spill/splash that can create aerosols and is loaded with bacteria.
BSL-2? Biosafety Level Two: moderate environmental health hazard. The organisms are associated with human diseases. Examples of the diseases are Salmonella, Clostridium difficile, Staphylococcus areus and Borrelia burgdorferi.
BSL-2 Agents and their routes? Agents: associated with human diseases. Routes: Percutaneous Injury, Ingestion, Mucous Membrane Exposure.
Examples of BSL-2 agents routes? 1.Percutaneous Injury: scratch, burn, and puncture. 2.Ingestion: swallowing, hands-to-mouth contact and splashing. 3.Mucous Membrane Exposure: eye, mouth and nose entrance
BSL-2 Practices? use BSL-1 (standard microbiological practice), Limited access, Biohazard warning signs, Sharps precaution and Biosafety manual that defines waste decontamination and medical surveillance.
BSL-2 Primary Barriers? Class 1-2 Biological Safety Cabinet and Personal Protective Equipment.
BSL-2 Facilities (secondary barrier )? Autoclave.
Created by: aneshia