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Chapter 12 - Purification and Separation
Term | Definition |
---|---|
Extraction | separates dissolved substances based on solubility in aqueous versus organic solvents; immicible liquids |
Filtration | separates solids from liquids |
Recrystallization | separates solids based on differential solubility; temp is impt cuz it should dissolve what we want with heat and solidify it with cool air but keep solvent dissolved at both temps |
Sublimation | solid to gas; separates solids |
centrifugation | separates large things based on mass and density |
distillation | separating liquids based on bp |
chromatography | stationary phase, mobile phase; separates compounds based on how tightly they adhere |
electrophoresis | separates macromolecules based on size and sometimes charge |
Isoelectric Focusing | separates proteins based on charge |