Nitrogen Metabolism Word Scramble

Embed Code - If you would like this activity on your web page, copy the script below and paste it into your web page.

Normal Size Small Size show me how

Question	Answer
N2 to NH4+ is	nitrogen fixation, nitrogenous
NH4 to NO2- is	nitrification, NITRITE reductase
NO2- to NO3-	nitrification 2.0, nitrATE reductase
NO3- to N2	Denitrification
Ammonia assimilation is carried out by _________ & ___________	glutamate synthase, glutamine synthetase
glutamate synthase is used in ______ & ______	bacteria and plants
glutamine synthetase occurs in ______, ________, and ______	glutamine synthetase
a-Ketogluturate + NH4 (using NADPH and ATP) converts to	glutamate
Glutamine and glutamate are ___________ carriers	nitrogen
________ moves amino groups between compounds	Transaminase
Is transamimation reversible	YES
Transamination requires ______	PLP (pyridoxal-5-phosphate)
What is the purpose of glutamine synthetase within assimilation	To mop up free nitrogen
Glutamine synthetase converts _______ to _______	glutamate to glutamine
Glutamate synthase converts ________ to ______	glutamine to glutamate
The net reaction of nitrogen assimilation is	a-ketoglutarate +NH4(+) +NADPH + ATP ---> glutamate + NADP+ + ADP + Pi
PLP is derived from _____	Vitamin B6 (pyridoxine)
What amino acid does PLP bond with to form the base	Lysine
What type of bond does PLP form with Lysine?	a covalent bond
What is the end product of this bond?	A schiff base (formed with and enzyme-lysine-PLP) Enzyme-PLP-schiff base
The enzyme-lysine-PLP formed would be considered a ______	prosthetic group (due to covalent bond)
Purpose of transaminase process	the goal is to transport an amine group from a-ketoglutarate to another a-keto acid
The enzyme-PLP-schiff base formed by PLP lysine and the enzyme serve as a ______ to transanimase	a coenzyme (a cofactor), due to the covalent bond, it is a prosthetic group
_____________ incorporates amine into a biomolecule	Glutamate synthase
_________ incorporates ammonia into a biomolecule	glutamine synthetase
Which of the two assimilation enzymes requires triphosphate molecules to work (like ATP, GTP, etc)?	glutamine synthetase
Which of the two assimilation enzymes DOES NOT require triphosphate molecules to work (like ATP, GTP, etc)?	glutamate synthase
Glutamate is a precursor to which amino acids?	Proline and arginine
Serine is a precursor to which amino acids?	glycine and then cysteine
______ is a carrier of one carbon units in amino acids and nucleotide during ________	Tetrahydrofolate, this happens during biosynthesis
phenylalanine is a precursor to which amino acid?	Tyrosine
serine is converted to glycine by what enzyme?	serine hydroxymethyl transferase
which enzyme converts phenylalanine to tyrosine	Phe-hydroxylase
Which amino acids are glucogenic?	All except Leucine and Lysine
Which amino acids are ONLY ketogenic?	Leucine and lysine
Which amino acids are BOTH ketogenic and glucogenic?	IPTTT, Isoleucine, Phenylalanine, Threonine, Tryptophan, Tyrosine
Pyruvate, Oxaloacetate, a-Ketoglutarate, Succinyl CoA and Fumerate are examples of which type of precursors?	Glucogenic precursors
Acetyl_CoA and Acetoacetate are examples of what type of precursors?	Ketogenic precursors
During catabolism of amino acids, which segments are created?	NH3+ breaks off from the rest of the amino acid (making it a a-keto acid)
What happens to the NH3+ broken off during amino acid catabolism?	It is used to make Urea
What happens to the rest of the amino acid after catabolism?	It is used to make glucogenic and ketogenic precursors
In amino acid catabolism, some amino acids are converted to glucogenic substrates via ______	transanimation
Which two amino acids can be converted to pyruvate?	Serine and cysteine, both of which have NH3 removed
Where does the urea cycle occur?	in the liver
On the cellular level, where does the urea cycle occur?	partly in mitochondria and partly in cytosol of cells
What is the main purpose of the urea cycle?	Disposal of nitrogen
What percent of nitrogen is disposed/excreted as urea?	80%
Which amino acids provide nitrogen for the urea cycle?	glutamate and aspartate (TATE)
What supplies carbon to the urea cycle?	Bicarbonate
In nucleotide metabolism, purine nucleotide synthesis yields _____ and then _____ and ______	IMP, then yields AMP and GMP
What activates glutamine PRPP amidotranferase?	PRPP
What inhibits glutamine PRPP amidotransferase?	it is inhibited by IMP, AMP, and GMP
A pyrimidine base is made up of what 3 components?	bicarbonate, Aspartate, and glutamine
in nucleotide metabolism, pyrimidine nucleotide synthesis yields what energy molecules?	UTP and CTP
First step of pyrimidine biosynthesis?

Created by: cdaughtry