Lab Test 1 part 1
Quiz yourself by thinking what should be in
each of the black spaces below before clicking
on it to display the answer.
Help!
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| Describe the position of your hands when carrying the microscope to and from your lab bench? | show 🗑
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| show | Answer: the limit of resolution of the unaided human eye is about .2 mm. for the typical light microscope, the limit is .2 um.
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| What two adjustments can be made to the condenser? what effect do these adjustments have on the image? | show 🗑
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| Why are condenser adjustments generally preferred over the use of light intensity control? | show 🗑
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| When using the oil immersion lens what four procedures can be implemented to achieve the maximum resolution? | show 🗑
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| Why is it advisable to start first with the low power lens when viewing a slide? | show 🗑
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| show | starting with the low power lens, which has a larger working distance, and progressing up to the oil lens is advised.
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| show | Answer: as the power of the objective lens increases the working distance decreases.
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| As the power of the objective lens increases the working distance decreases? | show 🗑
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| show | Answer: high dry
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| This objective lens provides the lowest magnification? | show 🗑
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| This objective lens has the shortest working distance? | show 🗑
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| show | Answer: low power
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| This lens collects and focuses light from the lamp onto the specimen on the slide? | show 🗑
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| show | Answer: ocular
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| show | Answer: ocular
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| A diaphragm is used to regulate light passing through this lens? | show 🗑
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| Acetone is the safest solvent for cleaning an objective lens. | show 🗑
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| show | True
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| The total magnification capability of a light microscope is only limited by the magnifying power of the lens system | show 🗑
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| show | False
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| Once focus is achieved at one magnification, a higher power objective lens can be rotated into position without fear of striking the slide. | show 🗑
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| show | Answer: the numerical aperture of the lens and the wavelength of light
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| The coarse and fine focus knobs adjust the distance between: | show 🗑
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| show | Answer: parfocal
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| show | Answer: 1000x
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| The most useful adjustment for increasing image contrast in low power magnification is: | show 🗑
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| Before the oil immersion lens is rotated into place, you should: | show 🗑
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| For which types of specimens is dark field microscopy preferred over brightfield microscopy? | show 🗑
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| show | Reflection of oblique rays off of objects passes through the lens system.
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| Staining cell is often performed to enhance images acquired by brightfield microscopy. Phase contrast microscopy does not require cell staining. Why is this advantageous? | show 🗑
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| show | Direct rays are produced when light passes straight through a transparent medium without changing amplitude or phase.
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| show | Diffracted rays are produced when light is bent during retardation by the medium due to density differences and are phase-shifted ¼ wavelength.
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| A phase contrast microscope differs from a bright field microscope by having a? | show 🗑
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| Which of the following would be the best observed for a bacterial cell using phase contrast microscopy? | show 🗑
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| The phase contrast microscope is best suited for observing? | show 🗑
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| What is quenching? | show 🗑
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| show | Direct exposure to light from mercury vapor arc lamps can damage eyes and the pressurized gas creates the potential to explode.
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| show | A darkfield condenser provides the best contrast and it deflects the majority of UV rays, which protects the viewer’s eyes.
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| Specific proteins on or in a cell can be tagged with fluorochromes that has been chemically linked to | show 🗑
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| show | heat filter, exciter filter, condenser, slide, objective lens and then the barrier filer.
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| show | screen out exciting light rays
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| how do you know your transfer to a broth was unsuccessful? how do you know your transfers in agar slants were successful? | show 🗑
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| show | Failure is no growth; or growth of a wide variety of colonies, signaling contamination.
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| provide three reasons why the use of aseptic technique is essential when handling microbial cultures in the lab | show 🗑
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| show | The flame from a Bunsen burner is used to sterilize transfer instruments (e.g., inoculating loop) and is used to flame the opening of the tube after the cap is removed and before the cap is replaced.
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| how is air contamination prevention when an inoculating loop is under to introduce or take bacteria sample to/from an agar plate? | show 🗑
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| show | Labels should be written on the bottom of the agar plate.
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| show | Agar plates should be incubated in an inverted position to prevent condensation on the agar surface that could spread the inoculated organisms.
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| disinfectants are effective against which types of organisms? which types of organisms may remain on the lab bench even after disinfection? What disinfectant is used in your lab? | show 🗑
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| show | The presence of growth in a liquid media such as a broth is indicated by turbidity or cloudiness. Individual colonies are not visible and one species could not be separated from another.
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| 7.2 what might be the advantages and disadvantages of using each type | show 🗑
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| a disinfectant is used on your work surface | show 🗑
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| show | remove and held with fingers of the loop hand
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| an inoculating loop or needle is sterilized using heat | show 🗑
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| which of the following would be a correctly labeled agar plate | show 🗑
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| show | Staining and microscopy or a streak plate can determine purity of a culture.
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| show | A colony is a visible microbial growth on a solid medium that originated from a single parent and through cell division produced a multitude of identical daughter cells.
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| what colony characteristics can be used for differentiation of bacterial species?as an exp compare the properties of colonies of serrate marcescens and micrococcus letters on your streak plate | show 🗑
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| why is dilution a necessary part of pure culture preparation? | show 🗑
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| show | The streak plate method does not require any additional media for dilution and only requires one plate for inoculation.
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| show | The pour plate method requires less skill, has optimization built in, and will more likely produce the desired result.
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| why is the loop flamed before it is placed in a culture tube? why is it flamed after completing the inoculation | show 🗑
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| 8.1 explain why plates should be inverted during incubation? | show 🗑
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| 8.2 explain why plates should be inverted during incubation? | show 🗑
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| how does smear preparation of cells from a liquid medium differs from preparation of cells from a. solid medium | show 🗑
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| why is it important to limit the quantity of cells used to prepare a smear | show 🗑
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| describe the potential consequences of making a smear that is too thick | show 🗑
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| show | Heat fixation causes cells to adhere to the slide during staining. However, structures such a capsules can undergo shrinkage during heat fixations and be lost in staining procedures. For that reason, capsule stains are not normally fixed.
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Review the information in the table. When you are ready to quiz yourself you can hide individual columns or the entire table. Then you can click on the empty cells to reveal the answer. Try to recall what will be displayed before clicking the empty cell.
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To hide a column, click on the column name.
To hide the entire table, click on the "Hide All" button.
You may also shuffle the rows of the table by clicking on the "Shuffle" button.
Or sort by any of the columns using the down arrow next to any column heading.
If you know all the data on any row, you can temporarily remove it by tapping the trash can to the right of the row.
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Created by:
zachflemings