Stack #159197
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| show | 5-20% O2
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| show | 5% O2
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| show | Presence or absence of O2
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| Aerotolerant anaerobe | show 🗑
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| Obligate anaerobe | show 🗑
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| show | 5-10% CO2
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| Anaerobes in respiratory tract | show 🗑
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| Anaerobes of the skin | show 🗑
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| Anaerobes of the genitourinary tract | show 🗑
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| show | Bifidobacterium; Eubacterium; Clostridium; Peptostreptococcus; Bacteroides fragilis group; Campylobacter; Fusobacterium; Porphyromonas; Prevotella; Sutterella; Veillonella; Biophila
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| show | 1. Trauma of skin or mucous membrane lining allowing bacteria entrance 2. Tissue w/ less O2 due to impaired blood flow; necrotic
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| 7 clues that an infectious process involves anaerobes when examining a specimen. | show 🗑
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| show | Stool, exudates from abscess collected by swab; voided midstream urine, vaginal swab
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| Acceptable specimens for anaerobic culture: | show 🗑
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| show | Needle & syringe; be sure to expel all air in syringes, transfer into sterile O2 & O2 free tubes.
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| show | Most wound cultures. O2 free swab for anaerobes; O2 free transport container w/ gel tube
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| How to collect and handle tissue. | show 🗑
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| How to collect and handle blood | show 🗑
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| Specimen integrity(container, time of collection) | show 🗑
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| show | Foul odor, necrotic tissue or exudate black, sulfur granules, bloody, purulent
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| show | Porphyromonas & Prevotella
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| 4 reasons to examine gram-stained smear of specimen | show 🗑
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| Media: Anaerobes have requirement for (3) | show 🗑
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| show | Pre-reduced anaerobically sterilized media. It is the best anaerobic media. Boil media to remove O2, autoclave & replace air w/ O2 free gas mixture.
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| 5 anaerobic medias are: | show 🗑
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| Anaerobic/Brucella Blood Agar | show 🗑
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| show | Porphyromonas spp.
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| Media: hemin - enhances growth of _______ | show 🗑
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| show | Primarily for B. fragilis. Supports growth of bile-tolerant Bacteroides spp; but, some Fusobacterium mortiferum, Klebsiella pnuemoniae, enterococci & yeast may grow limitedly.
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| show | Selective; contains gentomicin, 20% bile, and esculin. B. fragilis will turn the normally lt. yellow medium to brown do to hydrolysis.
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| show | Inhibits most aerobic organisms
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| show | Inhibits most anaerobes
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| Kanamycin-Vancomycin Laked Blood (KVLB) Grows: | show 🗑
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| show | Kanamycin, vancomycin, & lakes blood.
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| show | Inhibits most faculative gram neg. bacilli
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| show | Inhibits most g+ organisms & vancomycin-sensitive strains of Porphyromonas spp.
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| Laked Blood | show 🗑
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| show | Grows all obligate anaerobes & g+ faculative anaerobes. Selective & contains PEA.
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| Pheynylethyl alcohol | show 🗑
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| show | Virtually all bacteria. Obligate aerobes & microaerophiles near top, obligate anaerobes at the bottom & faculative anaerobes throughout.
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| show | backup source of culture material as obligate anaerobes can be easily overgrown and killed by faculative organisms present
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| Glove box | show 🗑
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| Gloveless anaerobic chamber | show 🗑
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| Anaerobic jars | show 🗑
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| show | Like a ziplock, contains same as jars. Catalist (alluminum pellets to remove O2), desicant, indicator, anaerobic gas
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| Catalyst (formula) | show 🗑
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| Indicator | show 🗑
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| show | C. difficile, Fusobacterium, Porphyromonas
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| show | Clostridium perfringens
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| Presumptive ID | show 🗑
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| show | Commercially available biochemical or enzyme based ID systems. Ex: Rapid ANA - enzyme based, 4 hrs, most clinically significant anaerobes
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| Clostridium tetani | show 🗑
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| Clostridium botulinum | show 🗑
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| show | G+ rod spore forming. Antibiotic assoc. pseudomembranous colitis. Transmission: part of normal intestinal flora, nonsocomial. Toxin: Toxin A (enterotoxin) affects metabolism of intestinal epithelium, loss of electrolyes & fluids. Toxin B (cytotoxin), acts
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| Clostridium perfringens | show 🗑
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| show | G+ rods terminal spores, drumstick shapeG+ rods terminal spores, tennis racket shapeG+ rods box-car shaped, spores difficult to detect
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| Egg Yolk agar | show 🗑
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| show | Selective/differential agar for C. difficile; yellow, ground glass colonies
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| show |
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