microbiology tests for gram negative/enteric bacteria
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| TSI | tripple sugar iron
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| TSI | pH indicator is phenol red, as well as ferric ammonium sulfate
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| TSI | screens for sugar metabolism (glucose and/or disacharide); acid production; hydrogen sulfide production
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| TSI | results of this test allow you to determine the presence of the enzymes sucrase and or lactase
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| A/A | yellow/yellow TSI results on top and in stab: initial use of glucoseand secondary use of one or both disacharides
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| K/A | red/yellow TSI results: initial use of glucose, but no disacharide use
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| K/K | red/red TSI results: no growth or aerobic non-fermenter
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| K | red color on TSI means productionof alkaline wastes from protien digestion
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| A | yellow color change on TSI results indicates acid production from sugar metabolism
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| TSI: bubbles and or cracking | gas production (CO2 or H2)
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| TSI results: black color | H2S production
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| Citrate | pH indicator is bromothymol blue
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| Citrate | screens for the presence of enzyme citrate permease and citrase, needed for metabolism of citrate
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| Citrate | determines if organism can use citrate as its sole metabolic (carbon) energy source
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| Citrate- bright blue | indicates organism has used citrate as a nutrient source and produced alkaline end products
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| Citrate-green color | organism couldnt use citrate, negative test
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| Durham tubes | phenol red is pH indicator
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| Durham tubes | screens for ability of an organism to use a particular sugar as a metabolic energy source; the production of gas
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| Durham tubes | production of acid OR alcohol end products
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| Durham tubes-lemon yellow | organism produces acids as fermentation end products
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| Durham tubes- red/orange | alcohol production from fermentation or aerobic respiration with no fermentation
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| Durham tubes-bubbles | gas production, primarily CO2
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| MR-VP | methyl red pH indicator; Berritts reagent added after incubation
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| MR-VP | screens for the end product of glucose metabolism:acetoin production or mixed acids
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| MR-VP- yellow tube | positive test, produced mixed acids
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| MR-VP- red ring after reagent added | production of acetoin from glucose metabolism
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| SIM deep | sulfer indole motility
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| SIM deep | detect for ability of organismto break down the amino acid cysteine; to break down the amino acid tryptophan; ability of organism to move in media
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| SIM deep | Kovac's reagent used
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| SIM deep- black precipitate | H2S production
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| SIM deep- red ring after Kovacs reagent | production of indole and a positive test, digestion of tryptophan
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| SIM deep- feathering away or cloudiness from stab line | positive test for motility, flagella present
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| Urea slant | phenol red pH indicator
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| Urea slant | determine if organism can break down urea or not, if urease is present
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| Urea slant- bright pink | positive for urea metabolism, breakdown of urea to ammonia, causing rise in pH
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| phenylaline slant | determine if organism can deaminate the amino group from the phenylaline amino acid
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| phenylaline slant | ferric chloride is the reagent
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| phenylaline slant- yellow color after ferrous chloride added | negative for phenylaline deamination
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| phenylaline slant- green color after ferrous chloride added | positive for the enzyme phenylaline deaminase
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| lysine/ornithine broth | bromocresol purple is pH indicator
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| lysine/ornithine broth | determines if organism has ability to decarboxylate of lysine OR ornithine
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| lysine/ornithine broth- purple | positive for lysine or ornithine metabolism: presence of lysine or ornithine decarboxylase enzyme
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| lysine/ornithine broth- yellow color | negitive for metabolism or ornithine or lysine
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