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Gram Negative Tests

microbiology tests for gram negative/enteric bacteria

QuestionAnswer
TSI tripple sugar iron
TSI pH indicator is phenol red, as well as ferric ammonium sulfate
TSI screens for sugar metabolism (glucose and/or disacharide); acid production; hydrogen sulfide production
TSI results of this test allow you to determine the presence of the enzymes sucrase and or lactase
A/A yellow/yellow TSI results on top and in stab: initial use of glucoseand secondary use of one or both disacharides
K/A red/yellow TSI results: initial use of glucose, but no disacharide use
K/K red/red TSI results: no growth or aerobic non-fermenter
K red color on TSI means productionof alkaline wastes from protien digestion
A yellow color change on TSI results indicates acid production from sugar metabolism
TSI: bubbles and or cracking gas production (CO2 or H2)
TSI results: black color H2S production
Citrate pH indicator is bromothymol blue
Citrate screens for the presence of enzyme citrate permease and citrase, needed for metabolism of citrate
Citrate determines if organism can use citrate as its sole metabolic (carbon) energy source
Citrate- bright blue indicates organism has used citrate as a nutrient source and produced alkaline end products
Citrate-green color organism couldnt use citrate, negative test
Durham tubes phenol red is pH indicator
Durham tubes screens for ability of an organism to use a particular sugar as a metabolic energy source; the production of gas
Durham tubes production of acid OR alcohol end products
Durham tubes-lemon yellow organism produces acids as fermentation end products
Durham tubes- red/orange alcohol production from fermentation or aerobic respiration with no fermentation
Durham tubes-bubbles gas production, primarily CO2
MR-VP methyl red pH indicator; Berritts reagent added after incubation
MR-VP screens for the end product of glucose metabolism:acetoin production or mixed acids
MR-VP- yellow tube positive test, produced mixed acids
MR-VP- red ring after reagent added production of acetoin from glucose metabolism
SIM deep sulfer indole motility
SIM deep detect for ability of organismto break down the amino acid cysteine; to break down the amino acid tryptophan; ability of organism to move in media
SIM deep Kovac's reagent used
SIM deep- black precipitate H2S production
SIM deep- red ring after Kovacs reagent production of indole and a positive test, digestion of tryptophan
SIM deep- feathering away or cloudiness from stab line positive test for motility, flagella present
Urea slant phenol red pH indicator
Urea slant determine if organism can break down urea or not, if urease is present
Urea slant- bright pink positive for urea metabolism, breakdown of urea to ammonia, causing rise in pH
phenylaline slant determine if organism can deaminate the amino group from the phenylaline amino acid
phenylaline slant ferric chloride is the reagent
phenylaline slant- yellow color after ferrous chloride added negative for phenylaline deamination
phenylaline slant- green color after ferrous chloride added positive for the enzyme phenylaline deaminase
lysine/ornithine broth bromocresol purple is pH indicator
lysine/ornithine broth determines if organism has ability to decarboxylate of lysine OR ornithine
lysine/ornithine broth- purple positive for lysine or ornithine metabolism: presence of lysine or ornithine decarboxylase enzyme
lysine/ornithine broth- yellow color negitive for metabolism or ornithine or lysine
Created by: hanalin2
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