Micro Lab Review
Help!
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| WEEK 7 | WEEK 7
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| What is the charge of DNA? | Negative
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| What is the formation of the Band due to? | Restriction site change of bacterial CHROMOSOME
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| What is the large band size due to? | New DNA, more nucleotides are present
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| Two to three band difference = how many genetic differences is how many genetic differences? | 1 genetic difference
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| What amount would be possibly related? | 4-6 genetic differences (2 genetic differences)
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| The direction of DNA migration is giong up or down? | DOWN
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| McConkey agar is what kind of media? | Selective and differential
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| What is it selective for? | Gram - organisms
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| What is it differential for? | Lactose fermentors and non-lactose fermentors
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| Blood agar is what kind of media? | Differential
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| Due to? | Hemolysis
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| What is TSA? | Neither selective nor differential
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| WEEK 9 | WEEK 9
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| What is a normal amt of neutrophils? | 40-75%
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| What is a normal amount of eosionophils? | 5%
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| What is a normal amt of basophils? | 0.5%
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| What is a normal amt of lymphocytes? | 20-35%
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| Normal Amt of monocytes? | 1-5%
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| If lymphocytes exceed 30%, what does this indicate? | Viral infection
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| Avid: | Stronger stick
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| Affinity: | Better stick
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| Sero testing, you are testing what with what? | Test known AB with unknown antigen
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| If the solution clumps, what does this mean? | Yes antigen is present
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| What DOESN'T the sed tube contain? | Serum
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| What does the fact that the sed rate is normal indicate? | No inflammation, it could still mean that patient is sick
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| What does the sed rate test for? | The rate of falling of RBC to indicate inflammation
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| What does a lot of eisonophils indicate? | Parasitic infection
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| What is a normal % for lymphocytes? | 20-35%
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| WEEK 10 | WEEK10
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| Biofilm allows the bacteria to do what? | Rapid growth, protects from Ab and phagocytossi
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| What about the glycocalyx? | EScape phagocytoris
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| What about fibrinogen-->fibrin00>clot? | Escape phagocytosis by using host
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| Digest DNA? | Overcome DNA nets of neutrophils
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| What is the purpose of the capsule stain? | Makes biofilm visible
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| What does the use of sanitizer do to the different kinds of transient and normal flora organisms on the skin and the number of organisms? | Decreases both
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| What about handwashing? | Handwashing decreases transient bacteria, but increases normal flora
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| WEEK 11 | WEEK 11
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| How do you differentiate between staph and strep? | Staph is catalaze +, strep is --
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| WEEK 12 | WEEK 12
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| Where do you look for hemolysis? | Around the ISOLATED COLONIES
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| QUICK VIEW | QUICK VIEW STREP TEST
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| What are you testing for? | Group A antigen
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| If it is present, what happens to (C OR T) | T becomes PINK
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| What does the C line test for? | It just tests for normal streptocooccal orgnsm everyone has
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| What color change incures in C? | BLUE
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| What does the agglutination look like? (IN ORDER) | Latex, Ab, then Antigen
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| WEEK 13 | WEEK 13
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| Indirect elisa detects patinet while direct Elisa detects patient | (blank)
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| What does the Hecktoen enteric agar test for? | Lactose fermeing vs. non-fermenting
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| McConkey agar differentiate for? | Lactose ferminging vs. non-fermienting
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| What color would the positive, lactose ferming stuff be ? | Purple
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| Why was it necessary to perform the reverse trascriptase? | Because PCR must be on DNA, norovirus has RNA
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| How is norovirus spread? | Through surfaces
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| WEEK 14 | WEEK 14
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| E-coli is diagnosed as? | Gram - lactose fermentor
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