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MICRO LAB
Micro Lab Review
| Question | Answer |
|---|---|
| WEEK 7 | WEEK 7 |
| What is the charge of DNA? | Negative |
| What is the formation of the Band due to? | Restriction site change of bacterial CHROMOSOME |
| What is the large band size due to? | New DNA, more nucleotides are present |
| Two to three band difference = how many genetic differences is how many genetic differences? | 1 genetic difference |
| What amount would be possibly related? | 4-6 genetic differences (2 genetic differences) |
| The direction of DNA migration is giong up or down? | DOWN |
| McConkey agar is what kind of media? | Selective and differential |
| What is it selective for? | Gram - organisms |
| What is it differential for? | Lactose fermentors and non-lactose fermentors |
| Blood agar is what kind of media? | Differential |
| Due to? | Hemolysis |
| What is TSA? | Neither selective nor differential |
| WEEK 9 | WEEK 9 |
| What is a normal amt of neutrophils? | 40-75% |
| What is a normal amount of eosionophils? | 5% |
| What is a normal amt of basophils? | 0.5% |
| What is a normal amt of lymphocytes? | 20-35% |
| Normal Amt of monocytes? | 1-5% |
| If lymphocytes exceed 30%, what does this indicate? | Viral infection |
| Avid: | Stronger stick |
| Affinity: | Better stick |
| Sero testing, you are testing what with what? | Test known AB with unknown antigen |
| If the solution clumps, what does this mean? | Yes antigen is present |
| What DOESN'T the sed tube contain? | Serum |
| What does the fact that the sed rate is normal indicate? | No inflammation, it could still mean that patient is sick |
| What does the sed rate test for? | The rate of falling of RBC to indicate inflammation |
| What does a lot of eisonophils indicate? | Parasitic infection |
| What is a normal % for lymphocytes? | 20-35% |
| WEEK 10 | WEEK10 |
| Biofilm allows the bacteria to do what? | Rapid growth, protects from Ab and phagocytossi |
| What about the glycocalyx? | EScape phagocytoris |
| What about fibrinogen-->fibrin00>clot? | Escape phagocytosis by using host |
| Digest DNA? | Overcome DNA nets of neutrophils |
| What is the purpose of the capsule stain? | Makes biofilm visible |
| What does the use of sanitizer do to the different kinds of transient and normal flora organisms on the skin and the number of organisms? | Decreases both |
| What about handwashing? | Handwashing decreases transient bacteria, but increases normal flora |
| WEEK 11 | WEEK 11 |
| How do you differentiate between staph and strep? | Staph is catalaze +, strep is -- |
| WEEK 12 | WEEK 12 |
| Where do you look for hemolysis? | Around the ISOLATED COLONIES |
| QUICK VIEW | QUICK VIEW STREP TEST |
| What are you testing for? | Group A antigen |
| If it is present, what happens to (C OR T) | T becomes PINK |
| What does the C line test for? | It just tests for normal streptocooccal orgnsm everyone has |
| What color change incures in C? | BLUE |
| What does the agglutination look like? (IN ORDER) | Latex, Ab, then Antigen |
| WEEK 13 | WEEK 13 |
| Indirect elisa detects patinet while direct Elisa detects patient | (blank) |
| What does the Hecktoen enteric agar test for? | Lactose fermeing vs. non-fermenting |
| McConkey agar differentiate for? | Lactose ferminging vs. non-fermienting |
| What color would the positive, lactose ferming stuff be ? | Purple |
| Why was it necessary to perform the reverse trascriptase? | Because PCR must be on DNA, norovirus has RNA |
| How is norovirus spread? | Through surfaces |
| WEEK 14 | WEEK 14 |
| E-coli is diagnosed as? | Gram - lactose fermentor |
Created by:
talkglitter2486