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Humoral Immune Responses

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Question
Answer
What are haptens?   small non-antigens that attach to large molecules and act as antigens, but can't stimulate anti-body response  
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What's bad about haptens?   Haptens can stick to our blood, so the antibodies might attack our cells  
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ANTIBODIES   ANTIBODIES  
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What is an eat me signal for antibdoies?   Opsination w/ complement or w/ binding  
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How many anitgens can an antibody see?   2 anitgens  
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STRUCTURES OF DIFFERENT ANTIBODIES   STRUCTURES OF DIFFERENT ANTIBODIES  
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What is the abbreviation for immunoglobulins?   Ig  
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IgG: based on p. 507   DESCRIBED  
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Strucutre:   Monomer, Y shaped  
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% of total serum antibody:   80 %  
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Location:   Blood, lymph, intestine  
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Half life in serum:   23 days  
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Complement fixation:   Yes  
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Placental transfer:   Yes  
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Function:   enhances phagocytosis, neutralizes tonxis and viruses, and protects fetus and newborn  
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Ig M   DESCRIBED  
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Strucutre:   Unique strucutre, pentamer?  
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% of total serum antibody:   5-10 %  
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Location:   Blood, lymph, B-cell surface as monomer  
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Half life in serum:   5 days  
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Complement fixation:   Yes  
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Placental transfer:   No  
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Function:   effective against microorganisms and agglutinating antigens, first antibodies made by b-cell  
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IgA:   DESCRIBED  
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Strucutre:   Dimer  
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% of total serum antibody:   10--15%  
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Location:   Secretions (tears, saliva, mucus, intestine, milk), blood, lymph  
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Half life in serum:   6 days  
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Complement fixation:   No, but some can  
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Placental transfer:   No  
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Function:   Localized protectioProtects mucous membrane  
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What is IgA deficient in?   Kids who didn't get breast milk  
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IgD   DESCRIBED  
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Strucutre:   Monomer  
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% of total serum antibody:   0.2  
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Location:   B cell surface, blood, lymph  
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half-life in serum   3 days  
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Complement fixation:   No  
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Placental transfer:   No  
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Function:   initation of immune response  
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IgE:   DESCRIBED  
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Structure:   Monomer  
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% of total serum antibody:   0.002  
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Location   Bound to mast and basophil cells in body, blood  
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Half life in serum:   2 days  
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Complement fixation:   No  
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Placental transfer:   No  
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Function:   Allergic reactions  
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FUNCTIONS OF DIFFERENT ANTIBODIES:   FUNCTIONS  
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Function of IgG:   Agglutination, complement fixation, and neutralization  
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What is agglutination?   binding to a lot and get them out in serum  
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When do we use neutralization?   When we wanna kill toxin  
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Function of IgM:   Agglutinization, complement fixation  
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Function of IgA:   Secretory antibody in mucous  
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Function of IgE:   Allergic response  
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Function of IgD:   Surface of b-cells  
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FUNCTIONS IN HOST DEFENSE   HOST DEFENSE  
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IgG:   Protects the tissue spaces  
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IgM:   Protects circulatory system  
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IgA:   Protects mucosal surfaces  
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IgE:   Induces allergic responses and protects from parasites like worms  
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IgD:   B-cell antigen detection  
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DEVELOPMENT OF ANTIBODY RESPONSE:   DEVELOPMENT  
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What are antigen receptors for the B lymphocyte?   IgD and M; they have to have them on surface  
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What is the process of selection of cells for antibody production?   1) stem cells make mature b-cells each with a specific sufrace Ig, 2. B cell complexes with antigen, 3. some memory cells form, 4. some b-cells make antibody producing plasma cells, 5. which secrete antibodies into blood circulation  
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IgM VERSUS IgG   READY...FIGHT!  
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Which appears first?   IgM  
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So what does IgG do?   Comes later, but sticks around longer  
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IMMUNE RESPONSE TO DISEASE   IMMUNE RESPONSE TO DISEASE  
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What is it due to? and what does it require? It timing vs the first response?   Requires IgG and is due to memory cells, and is quicker than when the first response happened  
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What is opsinization?   Coating something--eat me signal  
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What is neutralization?   Inactivation of virus or toxin  
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What does complement fixation infovle? What does it cause?   IgG and IgM; causes cell lysises  
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When does inflamation occur?   Secondary to complmeent activation  
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SUPERANTIGENS   SUPERANTIGENS  
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What is a superantigen?   A toxin that stimulates ALL B-cells, not just those with antibodies specific for that antigen  
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Why can't we use specific antibodies??   They are taken over by a sea of non-specific antibodies  
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What is more common, T cell superantigens or B-cell superantigens? Why?   T-cell; there's a limited space for B-cells and antibodies to be made  
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What are monoclonal antibodies?   When you isolate B-cells that make A SINGLE ANTIBODY.  
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