Microbiology Practical 1
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| Haemophilus influenzae Morphology, Media, Test | GN coccobacillus
ONLY grows on CHOC
Req. X and Y to grow
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| Haemophilus parainfluenzae Morphology, Media, Test | GN coccobacillus
ONLY grows on CHOC
Only req. V to grow
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| Haemophilus parahemolyticus Morphology, Media, Test | GN coccbacillus
ONLY grows on CHOC
Only req. V to grow, Hem+
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| Haemophilus paraphrohiles Morphology, Media, Test | GN coccbacillus
ONLY grow on CHOC
Only req. V to grow
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| Staphylococcus aureus Morphology, Media | GPC(clusters)
BAP=beta hem
MSA=+Ferm =Pink
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| Staphylococcus epidermidis Morophology, Media | GPC(clusters)
MSA=+Ferm=yellow
Non Beta Hem
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| Tests for Staphylococcus epidermis | Coag -
Susceptible to Novobiocin
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| Staphylococcus saprophyticus Morphology, Media | Non Beta Hem
Coag -
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| Test used for Staphylococcus saprophyticus | Coag -
Resistant to Novobiocin
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| Streptococcus pyogenes Morphology, Media | GPC (chains)
beta Hem on BAP (large zone)
Non beta Hem
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| Test for Streptococcus pyogenes Strep A | Susceptible to A disk (bacitracin)
PYR +
CAMP +
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| Streptococcus agalactiae Strep B Morphology, Media | GPC (chains)
beta Hem on BAP
small zone
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| Streptococcus pneumoniae Morphology, Media | GP (diplococci)
*alpha hem on BAP*
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| Test for Streptococcus pneumoniae | Bile esculin -
susceptible to P disk (optochin)
Bile solubility +
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| Enterococcus faecalis Morphology, Media | GPC (chains)shorts
gamma hem on BAP
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| Test for Enterococcus faecalis | Bile esculin +
Salt tolerance +
PYR +
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| Positive Bile esculin distinguishes which two organisms? | Enterococcus faecalis from Streptococcus pneumoniae
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| Streptococcus bovis Morphology, media | GPC (chains)
gamma hem on BAP
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| Salt tolerance distinguish between which two organisms? | Positive = Enterococcus faecalis
Negative = Streptococcus bovis
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| Viridans Streptococcus | GPC (chains) lots
alpha Hem on BAP
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| P disk (optochin) test distinguish between which two organisms? | Resistance = Viridans Strep
Susceptible = Streptococcus pneumoniae
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| Bile solubility test distinguishes which two organisms? | Positive = Streptocuccus pneumoniae
Negative = Viridans Strep
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| A disk (bacitracin) test distinguishes between which two organisms? | Resistance = Streptococcus agalactiae
Susceptible = Streptococcus pyogenes
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| Which organism doesn't go on BAP? | Neisseria gonorrhoeae
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| Neisseria gonorrhoeae Morphology, Media | Intercelluar diplococci
ONLY grows on CHOC
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| Neisseria gonorrhoeae Test used? | CTA glucose, Red NET test
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| Neisseria lactamica Gram stain results? | GN diplococci
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| Neisseria lactamica grows on what type(s) of agar? | CHOC, MTM, and BAP
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| Which organism only grows on CHOC and BAP? | Microcuccus catarrhalis
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| Which organism is an asaccharolytic? | Microcuccus catarrhalis
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| What organism has negative results for CTA test and NET test? | Microcuccus catarrhalis
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| Escherichia coli gram stain result? | GNR
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| Which organism is a positve fermenter on MAC and beta hemolytic on BAP? | Escherichia coli
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| What is the IMViC for E.coli? | ++--
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| What is the TSI result for E. coli? | A/A H2S-
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| What are the distinguishing characteristics for E. coli? | Flat colonies with green metallic colonies on EMB
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| How to distinguish E.coli(fermenter) from non fermenting E.coli? | Non fermenting E.coli does not ferment on MAC agar it appears clear and with the TSI the tube will show K/A
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| Enterobacter aerogenes IMViC ? | --++
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| Is Enterobacter aerogenes a fermenter? | No
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| What is the TSI result for E.aerogenes? | A/A
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| Which amino acid(s) does E. aerogenes result(s) is/are positive? | Lysine and Ornithine
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| Which amino acid(s) does E. pneumoniae result(s) is/are positive? | Lysine
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| Is E. pneumoniae a fermenter? | Yes on MAC
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| How does Proteus mirabilis look with gram staining? | GNR
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Created by:
MrsHo
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