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Micro Practical 1
Microbiology Practical 1
Question | Answer |
---|---|
Haemophilus influenzae Morphology, Media, Test | GN coccobacillus ONLY grows on CHOC Req. X and Y to grow |
Haemophilus parainfluenzae Morphology, Media, Test | GN coccobacillus ONLY grows on CHOC Only req. V to grow |
Haemophilus parahemolyticus Morphology, Media, Test | GN coccbacillus ONLY grows on CHOC Only req. V to grow, Hem+ |
Haemophilus paraphrohiles Morphology, Media, Test | GN coccbacillus ONLY grow on CHOC Only req. V to grow |
Staphylococcus aureus Morphology, Media | GPC(clusters) BAP=beta hem MSA=+Ferm =Pink |
Staphylococcus epidermidis Morophology, Media | GPC(clusters) MSA=+Ferm=yellow Non Beta Hem |
Tests for Staphylococcus epidermis | Coag - Susceptible to Novobiocin |
Staphylococcus saprophyticus Morphology, Media | Non Beta Hem Coag - |
Test used for Staphylococcus saprophyticus | Coag - Resistant to Novobiocin |
Streptococcus pyogenes Morphology, Media | GPC (chains) beta Hem on BAP (large zone) Non beta Hem |
Test for Streptococcus pyogenes Strep A | Susceptible to A disk (bacitracin) PYR + CAMP + |
Streptococcus agalactiae Strep B Morphology, Media | GPC (chains) beta Hem on BAP small zone |
Streptococcus pneumoniae Morphology, Media | GP (diplococci) *alpha hem on BAP* |
Test for Streptococcus pneumoniae | Bile esculin - susceptible to P disk (optochin) Bile solubility + |
Enterococcus faecalis Morphology, Media | GPC (chains)shorts gamma hem on BAP |
Test for Enterococcus faecalis | Bile esculin + Salt tolerance + PYR + |
Positive Bile esculin distinguishes which two organisms? | Enterococcus faecalis from Streptococcus pneumoniae |
Streptococcus bovis Morphology, media | GPC (chains) gamma hem on BAP |
Salt tolerance distinguish between which two organisms? | Positive = Enterococcus faecalis Negative = Streptococcus bovis |
Viridans Streptococcus | GPC (chains) lots alpha Hem on BAP |
P disk (optochin) test distinguish between which two organisms? | Resistance = Viridans Strep Susceptible = Streptococcus pneumoniae |
Bile solubility test distinguishes which two organisms? | Positive = Streptocuccus pneumoniae Negative = Viridans Strep |
A disk (bacitracin) test distinguishes between which two organisms? | Resistance = Streptococcus agalactiae Susceptible = Streptococcus pyogenes |
Which organism doesn't go on BAP? | Neisseria gonorrhoeae |
Neisseria gonorrhoeae Morphology, Media | Intercelluar diplococci ONLY grows on CHOC |
Neisseria gonorrhoeae Test used? | CTA glucose, Red NET test |
Neisseria lactamica Gram stain results? | GN diplococci |
Neisseria lactamica grows on what type(s) of agar? | CHOC, MTM, and BAP |
Which organism only grows on CHOC and BAP? | Microcuccus catarrhalis |
Which organism is an asaccharolytic? | Microcuccus catarrhalis |
What organism has negative results for CTA test and NET test? | Microcuccus catarrhalis |
Escherichia coli gram stain result? | GNR |
Which organism is a positve fermenter on MAC and beta hemolytic on BAP? | Escherichia coli |
What is the IMViC for E.coli? | ++-- |
What is the TSI result for E. coli? | A/A H2S- |
What are the distinguishing characteristics for E. coli? | Flat colonies with green metallic colonies on EMB |
How to distinguish E.coli(fermenter) from non fermenting E.coli? | Non fermenting E.coli does not ferment on MAC agar it appears clear and with the TSI the tube will show K/A |
Enterobacter aerogenes IMViC ? | --++ |
Is Enterobacter aerogenes a fermenter? | No |
What is the TSI result for E.aerogenes? | A/A |
Which amino acid(s) does E. aerogenes result(s) is/are positive? | Lysine and Ornithine |
Which amino acid(s) does E. pneumoniae result(s) is/are positive? | Lysine |
Is E. pneumoniae a fermenter? | Yes on MAC |
How does Proteus mirabilis look with gram staining? | GNR |