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Biotechnology Competition Division One 2010

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Question
Answer
Biotechnology   The use of living organisms or other biological systems in the manufacture of drugs or other products or for environmentalmanagement, as in waste recycling  
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Buffer   Solutions that have the capacity withstand the addition of small amounts of protons and/or hydroxide ions, or undergo dilution, without a dramatic change in pH.  
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DNA   deoxyribonucleic acid  
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Column Chromatography   The separation of mixtures into their constituents by preferential adsorption by a solid, as a column of silica  
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Size Exclusion Chromatography   (gel filtration) Chromatography in which the material to be fractionated separates primarily according to molecular size as it moves through a column of a gel and is washed with a solvent so that the fractions appear successively at the end of the column  
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Gel Filtration   (size exclusion) Chromatography in which the material to be fractionated separates primarily according to molecular size as it moves through a column of a gel and is washed with a solvent so that the fractions appear successively at the end of the column  
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Elution buffer   In column chromatography, it is put in the column to basically cleanse and lubricate. Generally, it helps to wash out any left-over proteins from a previous experiment. It can also help to separate the fractions that are collected.  
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Equilibrium buffer   The process of exposing a liquid to a gas that is at a certain partial pressure until the partial pressures of the gas inside and outside the liquid are equal.  
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Mobile Phase   In liquid chromatography, the phase that is moving in the bed, including the fraction of the sample held by this phase. The carrier gas in a gas chromatography procedure.  
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Stationary Phase   In chromatography, the nonmobile phase contained in the chromatographic bed. 
  
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Pipettor   Laboratory instrument used to transport a measured volume of liquid.  
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Protein   any of numerous, highly varied organic molecules constituting a large portion of the mass of every life form and necessary in the diet of all animals and other nonphotosynthesizing organisms  
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Epithelial Cells   Form the epithelial tissue that lines both the inner and outer surfaces of the body; serve a protective function and also aid in absorption and secretion.  
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Protein Primary Structure   Consists of the order in which amino acids are bonded to one another by peptide bonds.  
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Protein Secondary Structure   Involves the way that that chain of amino acids either twists or folds back upon itself to form either alpha helical, beta sheet or a variety of other possible arrangements.  
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Protein Tertiary Structure   Secondary structure in turn folds back and twists and bonds to itself in a three-dimensional manner  
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Precipitation   the precipitating of a substance from a solution  
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Solubility   the quality or property of being soluble; relative capability of being dissolved  
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Aqueous Phase   The water portion of a system consisting of two liquid phases, one that is primarily water and a second that is a liquid immiscible with water  
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Ethanol   Organic compound.Produced by fermentation, it is the intoxicating ingredient in alcoholic beverages. Ethanol for industrial purposes is made by either fermentation or chemical synthesis, then purified by distillation  
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Hydrophilic/Hydrophobi   Having a strong affinity for water  
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Diagnostic   Serving to identify or characterize; being a precise indication  
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