Carbohydrate Stains
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| This stain demonstrates reticulum fibers and basement membranes? | Periodic Acid Schiff
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| The principle of this stain is Oxidation by periodic acid forms aldehydes? | PAS
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| This stain demonstrates certain types of PAS+ mucosubstances secreted by the epithelia of various organs such as digestive tract, lungs and cervix? | PAS
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| This stain distinguishes a secreting adenocarcinoma (PAS+) from an undifferentiated squamous cell carcinoma (PAS-)? | PAS
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| This stain can, among other things demonstate fungus? | PAS
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| In the PAS stain, glycogen, Neutral mucosubstances, certain epithelial sulfomucins, sialomucins, colloidal material of the thyroid, pars intermedia of the pituitary, basement membrane and fungal walls all stain what color? | Bright Rose
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| What 3 carbohydrate stains fix with 10% NBF or Bouin's? | PAS, Alcian Blue pH2.5 and Alcian Blue Ph 1.0
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| All of the carbohydrate stains cut at what? | 4-5 microns
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| The control for a PAS stain is? | Kidney (cut 2 microns), cervix or liver containing glycogen. (4-5 microns)
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| Glutaraldehyde is not recommended as a fixative for this stain? | PAS
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| This stain demonstrates glycogen? | PAS with Diatase Digestion
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| The principle of this stain is that diastasse and alpha amylase depolymerase into smaller sugar units (maltose and glucose) that are washed out of the section? | PAS with Diastase Digestion
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| PAS with Diastase Digestion stains this color without digestion? | Bright Rose
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| What is the control for a PAS with Diastase Didestion stain? | Two sectiions of liver containing glycogen. One labeled "with" the other "without".
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| This stains Epithelial mucin? | Mayer Mucicarmine
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| This stain can be used to Cryptococcus neoformans but has to be replaced by staining with alcian Blue-PAS Technique? | Mayer Mucicarmine
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| The principle for this stain is that the aluminum forms a chelation complex binding the acid groups of the mucin and attaching the red color to the complex in a dye lake formation? | Mayer Mucicarmine
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| Small Intestine, Appendix and Colon are the control organs to use for what 4 carbohydrate stains? | Mayer Mucicarmine, Muller-Mowry, Alcian Blue Ph2.5 and Alcian Blue Ph1.0.
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| What color is the mucin (goblet cells) and capsule of cryptococcus in the Mayer Mucicarmine stain? | Deep Rose/Red
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| This stain demonstrates carboxylated and sulfated mucopolysaccharides and glycoproteins? | Muller-Mowry Colloidal Iron
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| The principle of this stain is the colloidal ferric ions are absorbed by the mucosubstances? | Muller-Mowry Colloidal Iron
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| Avoid chromatic fixative with this stain? | Muller-Mowry Colloidal Iron
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| The acid mucopolysaccharides and sialomucins in the Muller Mowry Colloidal Iron stain what color? | Deep Blue
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| The nuclei and cytoplasm in the Muller-Mowry Colloidal Iron stain what color? | Pink/red and pink
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| Chemically aldehdes or ketone dirivitives of the higher polyydric alcohols (containing more than one OH group)? | Carbohydrates
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| Often termed starch or sugars or as compounds that yeild compounds on hydrolysis? | Carbohydrates
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| Classification of carbohydrate that cannot be hydrolyzed into a simpler form? | Monosaccharides
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| Classification of carbohydrate that when hydrolyzed GIVES RISE TO TWO monosaccharides that can be the same or different molecules? | Disaccharides
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| Classification of carbohydrate that gives rise to MORE THAN TWO monosaccharides when hydrolyzed? | Polysaccharides (glycans)
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| Classification of carbohydrate that gives rise to ONE PRODUCE upon hydrolysis? | Homoglycans
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| Classification of carbohydrate that give RISE TO A MIXTURE of products? | Heteroglycan
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| The five classifications of Carbohydrates are? | Monosaccharides, Disaccharides, Polysaccharides (glycans), Homoglycans, Heteroglycan.
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| What are the four groups of Polysaccharides? | Neutral polysaccharides, acid polysaccarides, glycoproteins, glycolipids.
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| This stain demonstrates acid mucopolysaccarides? | Alcian Blue Ph 2.5
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| This stain demonstrates SULFATED mucopolysaccharides? | Alcian Blue Ph 1.0
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| This stain is used to differentiate epithelial and connective tissue MUCINS? | Alcian Blue with Hyaluronidase
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| This stain is used to differentiate between neutral and acid mucosubstances? | Alcian Blue-PAS-Hematoxylin
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| This stains BOTH sulfated AND carboxylated acid acid mucopolysaccharides and sialomucins? | Alcian Blue Ph 2.5
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| The aluminum in this stain forms a chelation complex binding the acid groups of the mucin and attaching the red color to the complex in a dye lake formation? | Mayer Mucimcarmine
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| QWhat color are the cryptococcus in the Mayer Mucicarmine stain? | Deep rose/red
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| Colloidal ferric ions are absorbed by mucosubstances in this stain? | Muller-Mowry Colloidal Iron
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| These two stain forms salt linkages with the acid group of the acic mucopolysaccharides? | Alcian Blue Ph 2.5 and Alcian Blue Ph 1.0
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| Staining will disappear or be dramatically reduced when tissue containing hyaluronic acid, chrondroitin sulfate A, or chrondroitin Sulfate C are digested with testicular hyaluronadase while glycoproteins (epithelial mucins) will not be affected? | Alcian Blue with Hyaluronidase
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| With this stain, acidic mucosubstances stain with the alcian blue and neutral mucosubstances are stained with the PAS reaction? | Alcian Blue-PAS-Hematoxylin
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| Umbilical cord is used as a control for this stain? | Alcian Blue with Hyaluronidase
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| Kidney or Mucin are the control needed for this stain? | Alcian Blue-PAS-Hematoxylin
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| Everything stained in the PAs stain is stained what color? | Bright Rose
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| In the PAS with Diastase Digestion, what are the staining results? (Wihout and With) | Without Digestion Bright Rose, With Digestion Absent
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