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Carbohydrate Stains

QuestionAnswer
This stain demonstrates reticulum fibers and basement membranes? Periodic Acid Schiff
The principle of this stain is Oxidation by periodic acid forms aldehydes? PAS
This stain demonstrates certain types of PAS+ mucosubstances secreted by the epithelia of various organs such as digestive tract, lungs and cervix? PAS
This stain distinguishes a secreting adenocarcinoma (PAS+) from an undifferentiated squamous cell carcinoma (PAS-)? PAS
This stain can, among other things demonstate fungus? PAS
In the PAS stain, glycogen, Neutral mucosubstances, certain epithelial sulfomucins, sialomucins, colloidal material of the thyroid, pars intermedia of the pituitary, basement membrane and fungal walls all stain what color? Bright Rose
What 3 carbohydrate stains fix with 10% NBF or Bouin's? PAS, Alcian Blue pH2.5 and Alcian Blue Ph 1.0
All of the carbohydrate stains cut at what? 4-5 microns
The control for a PAS stain is? Kidney (cut 2 microns), cervix or liver containing glycogen. (4-5 microns)
Glutaraldehyde is not recommended as a fixative for this stain? PAS
This stain demonstrates glycogen? PAS with Diatase Digestion
The principle of this stain is that diastasse and alpha amylase depolymerase into smaller sugar units (maltose and glucose) that are washed out of the section? PAS with Diastase Digestion
PAS with Diastase Digestion stains this color without digestion? Bright Rose
What is the control for a PAS with Diastase Didestion stain? Two sectiions of liver containing glycogen. One labeled "with" the other "without".
This stains Epithelial mucin? Mayer Mucicarmine
This stain can be used to Cryptococcus neoformans but has to be replaced by staining with alcian Blue-PAS Technique? Mayer Mucicarmine
The principle for this stain is that the aluminum forms a chelation complex binding the acid groups of the mucin and attaching the red color to the complex in a dye lake formation? Mayer Mucicarmine
Small Intestine, Appendix and Colon are the control organs to use for what 4 carbohydrate stains? Mayer Mucicarmine, Muller-Mowry, Alcian Blue Ph2.5 and Alcian Blue Ph1.0.
What color is the mucin (goblet cells) and capsule of cryptococcus in the Mayer Mucicarmine stain? Deep Rose/Red
This stain demonstrates carboxylated and sulfated mucopolysaccharides and glycoproteins? Muller-Mowry Colloidal Iron
The principle of this stain is the colloidal ferric ions are absorbed by the mucosubstances? Muller-Mowry Colloidal Iron
Avoid chromatic fixative with this stain? Muller-Mowry Colloidal Iron
The acid mucopolysaccharides and sialomucins in the Muller Mowry Colloidal Iron stain what color? Deep Blue
The nuclei and cytoplasm in the Muller-Mowry Colloidal Iron stain what color? Pink/red and pink
Chemically aldehdes or ketone dirivitives of the higher polyydric alcohols (containing more than one OH group)? Carbohydrates
Often termed starch or sugars or as compounds that yeild compounds on hydrolysis? Carbohydrates
Classification of carbohydrate that cannot be hydrolyzed into a simpler form? Monosaccharides
Classification of carbohydrate that when hydrolyzed GIVES RISE TO TWO monosaccharides that can be the same or different molecules? Disaccharides
Classification of carbohydrate that gives rise to MORE THAN TWO monosaccharides when hydrolyzed? Polysaccharides (glycans)
Classification of carbohydrate that gives rise to ONE PRODUCE upon hydrolysis? Homoglycans
Classification of carbohydrate that give RISE TO A MIXTURE of products? Heteroglycan
The five classifications of Carbohydrates are? Monosaccharides, Disaccharides, Polysaccharides (glycans), Homoglycans, Heteroglycan.
What are the four groups of Polysaccharides? Neutral polysaccharides, acid polysaccarides, glycoproteins, glycolipids.
This stain demonstrates acid mucopolysaccarides? Alcian Blue Ph 2.5
This stain demonstrates SULFATED mucopolysaccharides? Alcian Blue Ph 1.0
This stain is used to differentiate epithelial and connective tissue MUCINS? Alcian Blue with Hyaluronidase
This stain is used to differentiate between neutral and acid mucosubstances? Alcian Blue-PAS-Hematoxylin
This stains BOTH sulfated AND carboxylated acid acid mucopolysaccharides and sialomucins? Alcian Blue Ph 2.5
The aluminum in this stain forms a chelation complex binding the acid groups of the mucin and attaching the red color to the complex in a dye lake formation? Mayer Mucimcarmine
QWhat color are the cryptococcus in the Mayer Mucicarmine stain? Deep rose/red
Colloidal ferric ions are absorbed by mucosubstances in this stain? Muller-Mowry Colloidal Iron
These two stain forms salt linkages with the acid group of the acic mucopolysaccharides? Alcian Blue Ph 2.5 and Alcian Blue Ph 1.0
Staining will disappear or be dramatically reduced when tissue containing hyaluronic acid, chrondroitin sulfate A, or chrondroitin Sulfate C are digested with testicular hyaluronadase while glycoproteins (epithelial mucins) will not be affected? Alcian Blue with Hyaluronidase
With this stain, acidic mucosubstances stain with the alcian blue and neutral mucosubstances are stained with the PAS reaction? Alcian Blue-PAS-Hematoxylin
Umbilical cord is used as a control for this stain? Alcian Blue with Hyaluronidase
Kidney or Mucin are the control needed for this stain? Alcian Blue-PAS-Hematoxylin
Everything stained in the PAs stain is stained what color? Bright Rose
In the PAS with Diastase Digestion, what are the staining results? (Wihout and With) Without Digestion Bright Rose, With Digestion Absent
Created by: Pixel
 

 



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