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Quiz Two

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Question
Answer
Large, Gram-positive rods   Clostridium  
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Produce endospores   Clostridium  
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Anaerobic   Clostridium  
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Catalase-negative, oxidase-negative   Clostridium  
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Enriched media required for growth   Clostridium  
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Motile (except C. perfringens   Clostridium  
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Present in soil & alimentary tracts of animals & in feces   Clostridium  
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Neurotoxic Clostridia   Clostridium tetaniClostridium botulinum (types A - G)  
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Causative agent of tetanus   Clostridium tetani  
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Straight, slender, anaerobic, Gm +ve rod with special terminal endospores, giving characteristic “drumstick” appearance   Clostridium tetani  
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Endospores resistant to chemicals & boiling but killed by autoclaving at 121 deg. C for 15 mins   Clostridium tetani  
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Has swarming growth & hemolytic on blood agar   Clostridium tetani  
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Ten serologic types based on flagellar antigens   Clostridium tetani  
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Cross-neutralizing antibodies to neurotoxins between all serotypes   Clostridium tetani  
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Infection occurs by entry of endospores into traumatized tissues (abrasions & wounds   Clostridium tetani  
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Mode of action is by synaptic inhibition   Clostridium tetani  
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Incubation period is 5 to 7 days, may extend to 3 weeks   Clostridium tetani  
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Clinical effects of neurotoxins are similar in all domestic animals   Clostridium tetani  
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Nature & severity of clinical signs are dependent on anatomical site of the replicating bacteria, amount of toxin produced & species susceptibility   Clostridium tetani  
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Clinical signs include stiffness, localized spasms, altered facial expression, spasm of mastigatory muscles (“lock jaw”), generalized muscle stiffness (“saw-horse”) stance, especially in horses   Clostridium tetani  
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Recovered animals are not necessarily immune (toxin concentration that induce clinical disease is usually below threshold required to stimulate production of neutralizing antibodies   Clostridium tetani  
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Serious & fatal disease   Botulism  
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cause most outbreaks in domestic animals   C. botulinum types C and D  
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Inactivated by boiling for 20mins   C. botulinum  
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Gm +ve rod with sub-terminal endospores   C. botulinum  
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Occurs most commonly in waterfowl, cattle, horses, sheep, mink, poultry & farmed fish   C. botulinum  
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Pigs & dogs are relatively resistant & rare in domestic cats   C. botulinum  
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Poor quality baled silage & silage or hay containing rodent carcasses have been linked to outbreaks in horses & ruminants   C. botulinum  
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the most potent biological toxin known   Neurotoxins of C. botulinum  
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C. botulinum Mode of action is by   inhibition of neuro-muscular transmission  
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Botulism Clinical signs Develops   3 to 17 days after ingestion of toxin in all species of animals  
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Acute disease of cattle & sheep caused by C. chauvoei   Blackleg  
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bomasitis in sheep caused by C. septicum   Braxy  
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Manifests as cellulitis with minimal gas gangrene & gas formation   Malignant edema  
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Acute disease affecting sheep & occasionally cattle, caused by C. novyi type B   Infectious necrotic hepatitis  
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Occurs primarily in cattle & occasionally in sheep, caused by C. haemolyticum   Bacillary hemogl  
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Neuro disorder in newborn foals under 2 months, due to stress in dam, high level of corticosteroids in milk, high mortality   Shaker foal symptom  
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Cattle & Sheep: Gangrenous cellulitis & myositis caused by exotoxins, leading to rapid death   Blackleg  
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Large muscle masses of limbs, back & neck are frequently affected   Blackleg  
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Manifests as cellulitis with minimal gas gangrene & gas formation   Malignant edema  
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Clinical features of toxemia are similar to malignant edema   Gas gangrene  
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Hemoglobinuria: major clinical feature as a result of extensive red cell destruction   Bacillary hemogl  
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Histotoxic clostridia   Vaccination: Adjuvanted bacterin & toxoid is most effective  
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is the causative agent of Gas gangrene in human & domestic animals.   C. perfringens type A  
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C. perfringens type B   Lamb dysentery  
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Many animals die suddenly & high susceptibility of this group is attributed to the absence of microbial competition and the low proteolytic activity in the neonatal intestine   C. perfringens type B  
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Occurs in sheep at pasture, usually manifests as sudden death   perfringens type C:perfringens type C  
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Sudden death in goats & feedlot catle   Clostridia  
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Necrotic enteritis in chickens   Enteropathogenic & Enterotoxaemia-producing Clostridia  
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Haemorrhagic enteritis in neonatal pigs   Enteropathogenic & Enterotoxaemia-producing Clostridia  
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Neuro disorder in newborn foals under 2 months, due to stress in dam, high level of corticosteroids in milk, high mortality   Shaker foal symptom  
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Vax available   immunize dam and passive transfer of neutralizing antitoxin to foal, or give antitoxin serum to foal  
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