Minerals and Pigments
Quiz yourself by thinking what should be in
each of the black spaces below before clicking
on it to display the answer.
Help!
|
|
||||
|---|---|---|---|---|---|
| Ferric Iron | Fe 3+
Prussian Blue
(equal parts potassium ferrocyanide and Hydrochloric acid)
naturally found in small amounts in the bone marrow and spleen
🗑
|
||||
| Ferrous Iron | Fe 2+
Turnbull Blue
(potassium Ferricyanide w/ hydrochloric acid .4g to 40ml of .06N solution)
used in the schmorl technique
🗑
|
||||
| Prussian Blue | for ferric iron
equal parts potassium ferocyanide and hydrochloric acid
counterstain with nuclear fast red
🗑
|
||||
| Prussian Blue | Nuclei & Hemofuchsin.....BRIGHT RED
Hemosiderin (iron)....... BLUE
Background...................PINK
🗑
|
||||
| Turnbull Blue | for ferrous iron
ferrocyanide solution
wash in acetic acid
🗑
|
||||
| Turnbull Blue | Ferrous Iron...... BLUE
background......PINK/RED
🗑
|
||||
| Churukian-Schenk | method for argyrophil granules
demonstrates argyrophil granules in neurosecretory tumors
🗑
|
||||
| Churukian-Schenk | argyrophil and argentaffin....BLACK
nuclei......................RED
background................YELLOW/BROWN
🗑
|
||||
| Grimelius | for Argyrophil granules in neurosecretory tumors
hydroquinone and sodium sulfite used to reduce silver nitrate solution
🗑
|
||||
| Von Kossa | stain for calcium
Silver reacts with the anions (carbonate and phosphate) of calcium salts
a bright light is used to reduce the silver
🗑
|
||||
| Von Kossa | calcium salts........BLACK
background.........RED
🗑
|
||||
| Allzarin Red S | stain for calcium
place in allzarin red s solution and check progress microscopically
🗑
|
||||
| Allzarin Red S | calcium deposits...ORANGE/RED
🗑
|
||||
| Schmorl Technique | used to demonstrate reducing substances such as :
melanin, argentaffin granules, formalin pigment
ferric chloride-potassium ferricyanide produces turnbull blue precipitate
mayer mucicarmine
metanil yello
🗑
|
||||
| Schmorl Technique | reducing substances...........BLUE/GREEN
goblet cell-mucin..............ROSE
background.....................YELLOW-GREEN
🗑
|
||||
| Fontana Masson | demonstrates melanin and argentaffin granules
argentaffin granules reduce silver naturally
silver nitrate->gold chloride -> sodium thiosulfate -> nfr
🗑
|
||||
| Fontana Masson | Melanin.............................BLACK
Argentaffin Granules..........BLACK
Nuclei................................PINK
🗑
|
||||
| Rhodanine method | demonstrates copper
detects copper especially in liver as in Wilson's disease
rhodanine solution-> mayor hematoxilyn-> sodium borate
🗑
|
||||
| Rhodanine method | copper.......BRIGHT RED/YELLOW
nuclei.........LIGHT BLUE
🗑
|
||||
| Gomori Methenamine Silver | demonstrates urates as found in gout
deparaffinize and rinse with absolute alcohol->methenamine silver->sodium thiosulfate->light green counter
🗑
|
||||
| Gomori Methenamine Silver | urates.................BLACK
background........BLUE/GREEN
🗑
|
||||
| Hall Stain for Bile | demonstrates bilirubin and can distinguish bile pigment from other pgments
green color develops when the bilirubin oxidizes with ferric chloride in trichloroacetic acid medium called FOUCHET REAGENT
*counter with van giesen (acid fuchsin/picric acid)
🗑
|
||||
| Halll Stain for Bile | bilirubin................GREEN
background...........YELLOW
🗑
|
Review the information in the table. When you are ready to quiz yourself you can hide individual columns or the entire table. Then you can click on the empty cells to reveal the answer. Try to recall what will be displayed before clicking the empty cell.
To hide a column, click on the column name.
To hide the entire table, click on the "Hide All" button.
You may also shuffle the rows of the table by clicking on the "Shuffle" button.
Or sort by any of the columns using the down arrow next to any column heading.
If you know all the data on any row, you can temporarily remove it by tapping the trash can to the right of the row.
To hide a column, click on the column name.
To hide the entire table, click on the "Hide All" button.
You may also shuffle the rows of the table by clicking on the "Shuffle" button.
Or sort by any of the columns using the down arrow next to any column heading.
If you know all the data on any row, you can temporarily remove it by tapping the trash can to the right of the row.
Embed Code - If you would like this activity on your web page, copy the script below and paste it into your web page.
Normal Size Small Size show me how
Normal Size Small Size show me how
Created by:
burkan2
Popular Science sets