lab analysis final
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electrical conductivity that physically detects a clot | mechanical endpoint
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Used to measure light scattered at a 90 degree angle by particles in solution | nephelometry
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decrease in light flow detects clot formation utilizes what method | photo-optical endpoint
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process of generating photons of light via a chemical reaction | chemilluminescence
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methodology used in urinalysis dipstick, micro biochemical & routine chemistry testing | spectrophotometry
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separating complexes based on mobile phase and stationary phase | chromatography
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in electrophoresis, particles are separated on the basis of | net charge, size and shape
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optimal membrane choice for ISE measurement of pH | glass
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mechanical endpoint | coagulation studies
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spectrophotometry | urinalysis biochemical testing
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flow cytometry | automated cell differential
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electrical impedence | complete blood count
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flame photometry | concentrations of Na+, K+ and Li+
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measures analyte separated by chromotography | Mass spectrophotometry
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measure electromagnetic radiation | atomic absorption
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measures emitted light generate by a chemical | chemilluminescensce
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a holmium oxide filter is used to determine what | wavelength accuracy of a spectrophotometer
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coulter principle | electrical impedence
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photometry | measures light intensity without regard to wave length
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spectrophotometry | measures light intensity with regard to wavelength
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nephlometry | measures scattered light at a 90 degree angle
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Beer's law in spectrophotometry | relation of absorbance and transmittance to concentration
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potentiometry | electrochemical cell = 2 half cells
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labeled and unlabeled analyte introduced at same time and may attach to binding sites on the antibody | competitive immunoassay
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type of immunoassay that does not require separation of bound and free factions | homogenous
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value which occurs with the greatest frequency | mode
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calculated average of values | mean
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value at the center/midpoint fo the observation | median
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closeness of the agreement between the measured value of an analyte to its true value | accuracy
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bias in 1 direction that displaced the mean | shift
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gradual increase or decrease in QC values | trend
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FPIA | competitive
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MEIA | sandwich
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EMIT | competitive
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obtain the same result time after time | precision
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attraction of antibody/antigen pair, likelihood of binding | affinity
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after binding likelihood of separation | avidity
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separation techniques | adsorption, precipitation, solid phase
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adsorption | particles are added that trap antigen, labeled or unlabeled
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precipitation | environment is altered to affect solubility of protein by adding a substance
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solid phase | use to immobilize reagant antibody or antigen
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quality assurance | every aspect of the lab
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quality control | analytical piece
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maximum # of specimen that can be analyzed in a given time | throughput
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random access testing | any test can be run on any specimen
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sequential testing | series of tests are run on a sample
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continuous flow analyzer | all samples flow through a common reaction vessel
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centrifugal analyzer | reagent and sample in a cuvette on a rotor. Reaction occures by spinning
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discrete sample analyzer | each sample travels in its own reaction vessel
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substance that is dissolved in a liquid | solute
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to make a critical dilution and measurement one should use a | volumetric pipette
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A pipette with TC needs to be | blown out
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in the SI system temp is measured on what scale | Kelvin
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This type of microscopy is used to see unstained elements such as platelets | phase contrast microscopy
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temp at which water boils in Fahrenheit | 212 degrees
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type of plastic used in a laboratory | polystyrene and polycarbonate
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types of glass used in a laboratory | pyrex and borosilicate
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agency that oversees chemical purity | IUPAC
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