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clls-203 lab
lab analysis final
Question | Answer |
---|---|
electrical conductivity that physically detects a clot | mechanical endpoint |
Used to measure light scattered at a 90 degree angle by particles in solution | nephelometry |
decrease in light flow detects clot formation utilizes what method | photo-optical endpoint |
process of generating photons of light via a chemical reaction | chemilluminescence |
methodology used in urinalysis dipstick, micro biochemical & routine chemistry testing | spectrophotometry |
separating complexes based on mobile phase and stationary phase | chromatography |
in electrophoresis, particles are separated on the basis of | net charge, size and shape |
optimal membrane choice for ISE measurement of pH | glass |
mechanical endpoint | coagulation studies |
spectrophotometry | urinalysis biochemical testing |
flow cytometry | automated cell differential |
electrical impedence | complete blood count |
flame photometry | concentrations of Na+, K+ and Li+ |
measures analyte separated by chromotography | Mass spectrophotometry |
measure electromagnetic radiation | atomic absorption |
measures emitted light generate by a chemical | chemilluminescensce |
a holmium oxide filter is used to determine what | wavelength accuracy of a spectrophotometer |
coulter principle | electrical impedence |
photometry | measures light intensity without regard to wave length |
spectrophotometry | measures light intensity with regard to wavelength |
nephlometry | measures scattered light at a 90 degree angle |
Beer's law in spectrophotometry | relation of absorbance and transmittance to concentration |
potentiometry | electrochemical cell = 2 half cells |
labeled and unlabeled analyte introduced at same time and may attach to binding sites on the antibody | competitive immunoassay |
type of immunoassay that does not require separation of bound and free factions | homogenous |
value which occurs with the greatest frequency | mode |
calculated average of values | mean |
value at the center/midpoint fo the observation | median |
closeness of the agreement between the measured value of an analyte to its true value | accuracy |
bias in 1 direction that displaced the mean | shift |
gradual increase or decrease in QC values | trend |
FPIA | competitive |
MEIA | sandwich |
EMIT | competitive |
obtain the same result time after time | precision |
attraction of antibody/antigen pair, likelihood of binding | affinity |
after binding likelihood of separation | avidity |
separation techniques | adsorption, precipitation, solid phase |
adsorption | particles are added that trap antigen, labeled or unlabeled |
precipitation | environment is altered to affect solubility of protein by adding a substance |
solid phase | use to immobilize reagant antibody or antigen |
quality assurance | every aspect of the lab |
quality control | analytical piece |
maximum # of specimen that can be analyzed in a given time | throughput |
random access testing | any test can be run on any specimen |
sequential testing | series of tests are run on a sample |
continuous flow analyzer | all samples flow through a common reaction vessel |
centrifugal analyzer | reagent and sample in a cuvette on a rotor. Reaction occures by spinning |
discrete sample analyzer | each sample travels in its own reaction vessel |
substance that is dissolved in a liquid | solute |
to make a critical dilution and measurement one should use a | volumetric pipette |
A pipette with TC needs to be | blown out |
in the SI system temp is measured on what scale | Kelvin |
This type of microscopy is used to see unstained elements such as platelets | phase contrast microscopy |
temp at which water boils in Fahrenheit | 212 degrees |
type of plastic used in a laboratory | polystyrene and polycarbonate |
types of glass used in a laboratory | pyrex and borosilicate |
agency that oversees chemical purity | IUPAC |