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Microbiology

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Question
Answer
How did Griffith demonstrate a gene transfer?   capsular production could be restored to a capsular mutant from dead, wild-type bacteria  
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How did Avery show that genes are made of DNA?   bacterial substance that caused transformation DNase sensitive--provided strong evidence that transferal of DNA rather than protein/RNA was necessary for capsule production  
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What did Hershey and Chase's experiment confirm?   that DNA, rather than protein, is the genetic material  
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Gene   DNA coding material for a single protein  
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Chromosome   large circular molecule of several million base pairs of DNA that contains cellular genes  
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Genome   collection of all the genes in a cell  
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Complementary DNA   DNA that will base pair with another DNA molecule--A in place of T, C in place of G of the other molecule  
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How many genes might there be in an average bacterium? In a human cell?   bacterium--5,000==human cell--50,000  
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What cells have diploid or haploid genomes?   prokaryotic--haploid genomes--eukaryotic cells--diploid genomes  
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What must happen to the genetic material when division of a cell occurs?   when division of a cell occurs, genome must be duplicated  
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Where does DNA synthesis begin?   at the origin of replication  
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What is produced by DNA replication?   2 double-stranded DNA molecules identical to the starting DNA  
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How does DNA proofread?   through DNA polymerase--checks to see if newly added nucleotides are complementary to template--if not--DNA polymerase removes and replaces them with new nucleotide  
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Are human viruses harmful or beneficial?   can be bothe--whn virus causes damage to host it is harmful--when virus used by gene therapy to repair human genetic defect it is beneficial  
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What is a viral vector?   virus used in gene therapy to deliver a gene with proper activity to host cells that need the gene  
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