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TCC Nebauer Vocab 2

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Term
Definition
Conjugation   Transfer of plasmid DNA from donor to recipient via a pilus  
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Obligate anaerobe   Organisms for which oxygen is poison  
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Prophage   Inactive virus within a cell that often incorporates it's DNA into the host chromosome  
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Lagging Strand   Strand of DNA that is synthesized discontinuously in replication  
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Vector   Nucleic acid molecules used to introduce foreign DNA or gene into a cell  
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Southern Blot   Where DNA molecules are transferred to a nitrocellulose membrane for screening  
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Methylation   Addition of methyl groups to one or two bases of a specific nucleotide squence  
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Halophile   Organisms requiring high Salt environments  
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Mutagen   Physical or chemical agents that may alter the normal DNA swquence  
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Mesophile   Organisms that grow best at temperatures between 20 C and 40 C  
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Plaque   Clear zone on a lawn of bacteria that indicates viral replication  
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R Factors   Genes on plasmid's that provide antibiotic resistance  
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Recombinant DNA   Contains homologous segments of DNA from two different genomes (or organisms)  
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Transformation   A cell takes up naked DNA from the environment and incorporates it into it's genome  
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Recombination   Exchange of homologous segments of DNA  
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Hfr Strain   Where an F plasmid is integrated into the bacterial chromosome at a specific site  
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Gel Electrophoresis   Method used to isolate fragments of DNA based on their size and charge  
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Reverse transcriptase   Viral enzyme tha makes copies of DNA from RNA designated cDNA  
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Nucleotide analogs   Compounds that are structurally similar to normal nucleotides  
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Reactive oxygen species   Oxidizing agents that are poisonous to anaerobes  
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Frameshift mutation   Insertion or deletion of nucleotide base pairs to cause a change in reading frame  
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F Factor   Plasmid containing a gene for conjugation pilus  
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DNA Fingerprinting   Method used to identify individuals by their unique DNA swquences  
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Polymerase chain reaction   Technique used to produce large number of identical DNA molecules in vitro  
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Transduction   Involves transfer of bacterial DNA from one cell to another via a replication virus  
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operon   Consists of a promoter, and operator and a series of functional genes  
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Competence   The ability to take up DNA from the environment and undergo transformation  
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Inclusion Bodies   Signs of viral presence in infected cells see primarily in the host cell nucleus  
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Log Phase   Period during which bacteria undergo rapid growth and reproduction  
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Thymine Dimer   Adjacent thymine bases covalently bond to one another causing a mutation  
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Transposon   DNA that moves form one location to another on the same or other chromosome  
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Ames test   A fast, inexpensive method for screening mutagens  
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Represor protein   Molecule that binds an operator to stop transcription of mRNA  
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Chemoautotroph   Use organic compounds for energy and carbon dioxide as a carbon sourse  
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Facultative   Orgnaisms that can survive either aerobically or anaerobically  
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Photoheterotroph   Use light as an energy source and organic compounds as a carbon source  
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Gene probe   Labeled nucleic acid molecules of specific  
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Bacteriophage   A virus that infects bacteria  
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Promoter   Location on an operon to which RNA poymerase binds.  
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Lysogenic cycle   Replication cycle in which virally-infected host cells grow and reproduce before lysis  
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